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EC number: 410-560-1 | CAS number: 153519-44-9 CGL 400
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: well documented, GLP and guideline conform, some information to test material are not given (storage, expiration date of the batch), read across substance
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- GLP compliance:
- yes
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- 2-(4,6-diphenyl-1,3,5-triazin-2-yl)-5-((hexyl)oxy)phenol
- EC Number:
- 411-380-6
- EC Name:
- 2-(4,6-diphenyl-1,3,5-triazin-2-yl)-5-((hexyl)oxy)phenol
- IUPAC Name:
- 411-380-6
Constituent 1
Method
- Target gene:
- HGPRT
Species / strain
- Species / strain / cell type:
- Chinese hamster lung fibroblasts (V79)
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat liver S-9 microsomal activation system
- Test concentrations with justification for top dose:
- 0, 18.52, 55.56, 166.67 and 500 microg/ml
- Vehicle / solvent:
- DMSO
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- N-dimethylnitrosamine
- Remarks:
- without metabolic activation
Migrated to IUCLID6: DMN, 1.0 microliter/ml
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- Remarks:
- with metabolic activation
Migrated to IUCLID6: 0.3 microliter/ml
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Exposure duration: with metabolic activation for 5 hours and in the experiment without metabolic activation for 21 hours
SELECTION AGENT (mutation assays): 6-Thioguanine
NUMBER OF REPLICATIONS: two
NUMBER OF CELLS EVALUATED: all colonies were counted
DETERMINATION OF CYTOTOXICITY
Cytotoxicity test
In the part with metabolic activation, at the highest concentration of 500 microg/ml an acute growth inhibiting effect of 55.15% could be seen. Withoutmetabolic activation treatment was growth inhibiting by 39.42% at the highest concentration of 500 microg/ml. Accordingly, 500 microg/ml was chosen as the highest concentration for the first mutagenicity assay with and without metabolic activation. Precipitates of the test compound were visible in the cultures down to the concentration of 62.5 ng/ml.
OTHER
The cells have a stable karyotype with a modal chromosome number of 22±1. All stock cells were checked for mycoplasma contamination, using the Hoechst- Dye staining method or the 6-MPDR method, before being frozen. - Evaluation criteria:
- All mutant frequencies are normalized to a virtual cloning efficiency of 100% at the end of the expression
period. If the cloning efficiency of the viability cultures is lower than 15%, the corresponding
mutant frequency is usually not calculated, owing to the high statistical insignificance of the
result. For every concentration a mean mutant factor, which is defined as the ratio of the mean mutant
frequencies of the treated cultures with the mean mutant frequencies of the solvent control cultures,
will be calculated. - Statistics:
- Statistical significance of mutant frequencies was carried out according to the UKEMS guidelines
Results and discussion
Test results
- Species / strain:
- Chinese hamster lung fibroblasts (V79)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: Precipitates of the test compound were visible in the cultures down to the concentration of 62.5 microg/ml.
RANGE-FINDING/SCREENING STUDIES:
COMPARISON WITH HISTORICAL CONTROL DATA: Historical control data from gene mutation tests with Chinese Hamster Cells V79
ADDITIONAL INFORMATION ON CYTOTOXICITY: In the part with metabolic activation, at the highest concentration of 500 microg/ml an acute growth inhibiting effect of 55.15% could be seen. Without metabolic activation treatment with the test item was growth inhibiting by 39.42% at the highest concentration of 500 microg/ml. Accordingly, 500 microg/ml was chosen as the highest concentration for the first mutagenicity assay with and without metabolic activation. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Based on the results of two independently performed experiments and under the given experimental conditions, it is concluded that the test item and its metabolites did not show any mutagenic activity in this forward mutation system.
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