Registration Dossier
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EC number: - | CAS number: -
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guidleine study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Colony forming units (CFU): 1.5 x 10E9 CFU/L
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 30 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Test temperature: 20.5-21.5°C
- pH: 7.98 to 7.51
- pH adjusted: no
TEST SYSTEM
- Culturing apparatus: 500 mL brown glass bottles
- Measuring equipment:
OxiTop'" OC110 controller, WTW
OxiTop'" measuring heads, WTW
Stirring platform , WTW
Incubator, RUBARTH
pH-Meter, Multi 350i,WTW
Thermohygrograph, LUFFT
Analytical balance, SARTORIUS
Balance, EW 3000-2M, KERN
SAMPLING
- Sampling frequency: the oxygen consumption was determined continously during the 28 days incubation period
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 28
- Sampling time:
- 28 d
- Details on results:
- Both test item replicates reached the 10 % level (beginning of biodegradation) after 2 days, but failed to reach the pass level of 60 % biodegradation.
The mean biodegradation on day 28 was 28 %
BOD5 / COD results
- Results with reference substance:
- In order to check the activity of the test system sodium benzoate was used as functional control. The pass level> 60 % was reached after 1 day. The biodegradation rate reached a maximum of 100 % degradation on day 17.
Any other information on results incl. tables
In the toxicity control containing both test and reference item 65 % degradation occurred within 14 days. After 28 days the biodegradation came to 69 %. The degradation of the reference item was not inhibited by the test item.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item is classified as not readily biodegradable in the 10-d-window and after 28 days
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