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EC number: 807-137-2 | CAS number: 110528-94-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: All methods followed were based on the latest edition of "Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms" (EPA- 821-R-02-012) and BAL's standard operating procedure.
- Deviations:
- not applicable
- Principles of method if other than guideline:
- Bio-Analytical Laboratories (BAL), Doyline, Louisiana conducted a 48-hour acute definitive range find ing study on the sample known as ColaMoist 200 for RespirTek Incorporated, Biloxi, Mississippi for their client Colonial Chemicals Inc The test organism used was the fathead minnow, Pimephales
promelas. The test endpoint is the Lethal Concentration (LC50), which is the effluent concentration at which 50 percent of the test organisms die. - GLP compliance:
- not specified
Test material
- Reference substance name:
- 2-hydroxy-N,N'-bis(2-hydroxyethyl)-N,N,N',N'-tetramethylpropane-1,3-diaminium dichloride
- EC Number:
- 807-137-2
- Cas Number:
- 110528-94-4
- Molecular formula:
- 2-hydroxy-N,N'-bis(2-hydroxyethyl)-N,N,N',N'-tetramethylpropane-1,3-diaminium dichloride
- IUPAC Name:
- 2-hydroxy-N,N'-bis(2-hydroxyethyl)-N,N,N',N'-tetramethylpropane-1,3-diaminium dichloride
- Test material form:
- other: 70% w/w aqueous solution, equivalent to 70% active material.The removal of water is not deemed possible without having to apply temperatures that cause some degradation of the product. Water free substance is not appropriate for this test.
- Details on test material:
- Upon arrival, the sample was logged in and given an identification number. The sample was weighed on an analytical balance and added to the dilution water to obtain the mg/L dilutions needed.
Constituent 1
Test organisms
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- The fathead minnows were raised in-house and were approximately 1 - 3 days old at test initiation. The minnows were acclimated to test temperat ure and d i lution water hardness prior to test i ni t iation. Forty-eight hou r reference toxicant tests, usi ng sodium chloride (NaCl), were conducted in order to document organism sensi tivity.
Moderately-hard reconstituted water made per EPA guidelines was used as the dilution water and the control for the toxicity tests.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Moderately hard as required by the EPA guideline.
- Test temperature:
- 25±1°
- pH:
- Measured but not detailed in report
- Dissolved oxygen:
- Measured but not detailed in report
- Salinity:
- Not saline
- Details on test conditions:
- A series of range finding tests were conducted until an LC50 value could be obtained. At the conclusion of the study, the following test concentrations were used: 20,000, 10,000, 5,000, 2000, I 000, 500, 250, 200, 125, 100, 50, 25 and 12.5 milligrams/Liter (mg/L). The tests were conducted using two replicates of ten animals each for a total of 20 animals per concentration.
The tests were run in either a Precision R dual programmable illuminated incubator set at a temperature of 25±1°Celsius, or in a water bath using a Remcor heated liquid circulator to maintain a temperature range of 25± 1°Celsi us. A EMCR data loggers were used to monitor diurnal temperature throughout the testing period. Light cycles and intensities were recorded twice a month. - Reference substance (positive control):
- yes
- Remarks:
- sodium chloride - 48 hour reference tests to determine the animals sensitivity.
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4 729 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Applicant's summary and conclusion
- Conclusions:
- The sample of Cola Moist 200, as received was found to be lethally toxic to the Pimephales promelas test organisms after 48 hours of exposure.
The 48-hour LC50 value for this study was 4728.71 mg/L or 4.73 g/L .
At a received concentration of 70% by weight in water, and assuming that the active ingredient alone was responsible for the deaths, extrapolating back to 100% active material, the LC50 would be calculated as 3310 mg/l.
The time used for the test was 48 hours rather than the normal 96 hours under current OECD guidelines. Extrapolation to a longer exposure time is not necessarily linear and thus the lethal dose values required for classification cannot be responsibly calculated.
However, it would suggest that ColaMoist is not considered to be toxic to fish.
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