Carbonic acid, ethyl methyl ester

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: official guideline method, GLP with certificate, good documentation, identity of substance clear but no details on its purity

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

433-480-9

EC Name:

-

Cas Number:

623-53-0

Molecular formula:

C4H8O3

IUPAC Name:

ethyl methyl carbonate

Details on test material:

Test material as stated in study report ("Sponsor's identification"): MEC
Description: clear colourless liquid
Batch number: 00925
Date received: 11.27.2000
Storage conditions: room temperature, in the dark until 01.16.2001, thereafter at +4 °C in the dark

Remark of the author of this IUCLID dossier concerning the substance identity:
Every page of the copy of the study report contains an annotation referring to the data sharing agreement in place between the licensee that uses this study report and the data owner. In this data sharing agreement the identity of the chemical substance is specified with "Ethyl Methyl Carbonate - EC No. 433-480-9, CAS Nr. 623-53-0".

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Test sample was diluted with acetone to give a theoretical concentration of 20 mg/L

Test solutions

Details on test solutions:

Standard solutions of test material were prepared in acetone at a nominal concentration of 20 mg/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

1st instar Daphnia magna derived from in-house laboratory cultures
10 daphnids per test vessel.

Feeding: algae, specifically Chlorella sp.
Age daphnids used in test: < 24 h

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Remarks on exposure duration:

Immobilisation or adverse reactions to exposure were recorded at 24 h and 48 h after the start of exposure

Test conditions

Hardness:

approximate theoretical total hardness of 250 mg/L as CaCO3

Test temperature:

21 °C, water temperature was recorded daily throughout the study

pH:

7.8 +/- 0.2

Dissolved oxygen:

The reconstitued water was aerated until the dissolved oxygen concentration was approximately air-saturation value

Details on test conditions:

The test vessels maintained in a temperature controlled room at 21 °C with a photoperiod of 16 h light and 8 hours of darkness with 20 minute dawn and dusk transition periods

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

, which was dissolved in reconstituted water

Basis for effect:

other: immobilisation and any adverse reactions

Details on results:

No immobilisation or adverse reactions to exposure were observed at all test concentrations.

Applicant's summary and conclusion

Conclusions:

The acute toxicity of ethyl methyl carbonate on the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EC50 of greater than 100 mg/L. Correspondingly the No Observed Effect Concentration was 100 mg/L.

No classification is required for acute aquatic toxicity according to REGULATION (EC) No 1272/2008 (of 16 Dec 2008) based on this test result.