Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-762-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July - August 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: This robust summary has a reliability rating of 2 because the study followed a standard guideline and was conducted without deviations that would invalidate the study. However, the study did not follow GLP guidelines.
- Justification for type of information:
- A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Cross-reference
- Reason / purpose for cross-reference:
- read-across: supporting information
Reference
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- July - August 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: This robust summary has a reliability rating of 2 because the study followed a standard guideline and was conducted without deviations that would invalidate the study. However, the study did not follow GLP guidelines.
- Justification for type of information:
- A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: OECD guideline 202 section 2: Effects on biotic systems (1984)
- Deviations:
- yes
- Remarks:
- The deviation did not impact the scientific integrity of the study
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Samples of the control and the Water Accomodated Fractions (WAFs) prepared on each loading rate were taken on days 0, 2, 5, 7 and 14 from the stirring vessels and at termination (composite of replicates) for analysis.
- Vehicle:
- no
- Details on test solutions:
- The test solutions were prepared as WAFs (water accomodated fractions). Individual WAFs were prepared by adding the appropriate amount of the test substance to 3.5 L of dilution water in a 4 L size glass aspirator bottle. The mixtures were stirred for approximately 24 hours on a magnetic stirplate with a Teflon coated stirbar. Stirring was initiated using a vortex of <10% of the static liquid depth (1.5 cm). Each vesesl was stoppered with a foil covered neoprene stopper. All mixtures appeared clear during the stirring period.
After stirring, WAFs were removed through the outlet at the bottom of the vessel and added to test chambers. New WAF solutions were prepared for each 24-hour renewal. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: cultured in the Environmental Toxicology and Chemistry Laboratory of Exxon Biomedical Sciences, INC.
- Age of parental stock: 16 days
- Feeding during test
- Food type: Selenastrum capricornutum (algae)
- Amount: 2.32*10^5 cells/mL on days 0-6 and 1.56*10^5 cells/mL on days 7-21
- Frequency: daily
ACCLIMATION
- Acclimation period: no acclimation period (daphnia <= 24 hours old)
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES
Young were observed, counted and removed daily. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21
- Remarks on exposure duration:
- d
- Post exposure observation period:
- No post exposure period
- Hardness:
- No data
- Test temperature:
- Mean test temperature (water): 20°c (sd=0.05), continuously monitored by computer in test area.
- pH:
- 6.6 TO 7.8
- Dissolved oxygen:
- 5.2 TO 9.3 mg/L
- Salinity:
- Freshwater study
- Nominal and measured concentrations:
- Range finding test: Nominal concentrations (loading rates): 50, 10, 2.5, 1 and 0.5 mg/L. Samples of the control and the 50 mg/L loading were analyzed for the test substance by gas chromatography with flame ionization detection (GC-FID). The results of the analysis showed that the levels of the test susbtance were below the practical quantitation limit of 0.2 mg/L.
Definitive test: 0, 1, and 5 mg/L (nominal concentrations = loading rates) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: closed (flasks stoppered with foil covered neoprene stoppers on days 0-6; flasks covered with plexiglass sheets on days 7-21)
- Material, size, headspace, fill volume: 500 mL size glass erlenmeyer flasks, containing approximately 560mL of solution on days -6 (no head space) and 500 mL solution on days 7-21; test chambers rinsed with reverse osmosis water after renewals to remove food substrates
- Aeration: no aeration
- Renewal rate of test solution: renewal of the test solutions was performed on Monday, Wednesday and Friday by transferring the adult daphnids to new solutions via pipette.
- No. of organisms per vessel: range-finding test: 5; definitve test: 10
- No. of vessels per concentration (replicates): range-finding test: 2 (two trials); definitive test: 4
- No. of vessels per control (replicates): range-finding test: 2 (two trials); definitive test: 4
- Biomass loading rate: definitive test: 1 daphnid per approximately 50 to 56 mL of solution
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: BW3 supplemented with vitamin B12 and selenium
- Culture medium different from test medium: no
- Intervals of water quality measurement: The quality of the dilution water used in culture and testing is monitored at weekly, monthly, semi-annual and annual intervals.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h ightL/ 8 h Dark with gradual intensity conversion between periods
- Light intensity: approximately 630 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Adult mortality / immobilization
- Percent reduction in reproduction (based on cumulative young per adults)
RANGE-FINDING STUDY
- Test concentrations: 0, 0.5, 1.0, 2.5, 10, and 50 mg/L
- Loadings used for the definitive study: 0, 1, and 5 mg/L - Reference substance (positive control):
- no
- Duration:
- 21 d
- Dose descriptor:
- EL50
- Effect conc.:
- > 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (loading rate)
- Basis for effect:
- reproduction
- Remarks:
- cumulative average young per adult
- Duration:
- 21 d
- Dose descriptor:
- EL50
- Effect conc.:
- > 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (loading rate)
- Basis for effect:
- immobilisation
- Remarks on result:
- other: (adults)
- Duration:
- 21 d
- Dose descriptor:
- NOELR
- Effect conc.:
- 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (loading rate)
- Basis for effect:
- reproduction
- Remarks:
- cumulative average young per adult
- Duration:
- 21 d
- Dose descriptor:
- NOELR
- Effect conc.:
- 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (loading rate)
- Basis for effect:
- immobilisation
- Remarks on result:
- other: (adults)
- Details on results:
- The first brood were observed in the control and each loading after 7 days. By day 14 a cumulative average of 20 young per female were produced in the control. A cumulative average of 54 young per female were produced in the control by day 21.
Other details on results in §Remarks on results including tables and figures. - Results with reference substance (positive control):
- No reference susbtance
- Reported statistics and error estimates:
- The No Observed Effect Loading Rate (NOELR) was determined using Dunnett's procedure of SAS.
- Validity criteria fulfilled:
- yes
- Remarks:
- Dissolved oxygen fell below 60% sat. on day 12 in old solution. Since treatment was renewed, not believed to have effect on study. At day 21, 54 young per adult rather than 60, but believed sufficient young were produced for evaluation.
- Conclusions:
- The 21-day EL50s based on adult immobilization and reduction in reproduction were >5 mg/L. The 21-day No Observed Effect Loading Rates (NOELRs) based on adult immobilization and reduction in reproduction was 5 mg/L.
- Executive summary:
The 21-day EL50s based on adult immobilization and reduction in reproduction were >5 mg/L loading. The 21-day No Observed Effect Loading Rates (NOELRs) based on adult immobilization and reduction in reproduction was 5 mg/L.
The study was performed to evaluate the toxicity of the water accomodated fraction (WAF) of the test substance to the daphnid, Daphnia magna, in a semi-static system for a 21-day period. The loading rates for this test were 1 mg/L and 5 mg/L. A control of laboratory dilution water was also tested. Individual WAFs were prepared by adding the appropriate amount of the test substance to 3.5 liters of BW3 in a 4 L size glass aspirator bottle. The mixtures were then stirred for approximately 24 hours on a magnetic stirplate with a Teflon coated stirbar. Stirring was initiated using a vortex of <10% of the static liquid depth (1.5 cm). Each vessel was stoppered to prevent loss of volatiles. After stirring, the WAF was removed from the outlet at the bottom of the vessel.
Four replicates chambers were prepared per treatment by completely filling the test chambers with WAF (no headspace). Samples were removed from each WAF and the control on day 0, 2, 5, 7, 14, and at termination (composite of the replicate test chambers) for chemical analysis by gas chromatography with flame ionization detection (GS-FID). The results of the analysis showed that the levels of the test substance were below the practical quantitation limit of 0.2 mg/L.
Table 1. Definitive test: Percent adult mortality / immobilization and percent reduction in reproduction (based on cumulative youngs per adult) for the 14- and 21-day period.
Loading rate (mg/L) |
% Adult Mortality / Immobilization |
% Reduction in reproduction |
||
14 days |
21 days |
14 days |
21 days |
|
Control |
7.5 |
17.5 |
N/A |
N/A |
1.0 |
10 |
12 |
0 |
9 |
5.0 |
10 |
25 |
25 |
28 |
N/A - Not applicable, % reduction in reproduction as compared to the controls.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD guideline 202 section 2: Effects on biotic systems (1984)
- Deviations:
- yes
- Remarks:
- The deviation did not impact the scientific integrity of the study
- GLP compliance:
- no
Test material
- Reference substance name:
- Hydrocarbons, C13-C16, isoalkanes, cyclics, <2% aromatics
- Molecular formula:
- None available - not a single isomer
- IUPAC Name:
- Hydrocarbons, C13-C16, isoalkanes, cyclics, <2% aromatics
- Details on test material:
- Hydrocarbons, C13-C16, containing isoalkanes and cyclics with less than 2% aromatics
- Substance type: UVCB
- Physical state: Clear liquid
- Analytical purity: 100% Commercial product
- Storage condition of test material: stored at room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples of the control and the Water Accomodated Fractions (WAFs) prepared on each loading rate were taken on days 0, 2, 5, 7 and 14 from the stirring vessels and at termination (composite of replicates) for analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test solutions were prepared as WAFs (water accomodated fractions). Individual WAFs were prepared by adding the appropriate amount of the test substance to 3.5 L of dilution water in a 4 L size glass aspirator bottle. The mixtures were stirred for approximately 24 hours on a magnetic stirplate with a Teflon coated stirbar. Stirring was initiated using a vortex of <10% of the static liquid depth (1.5 cm). Each vesesl was stoppered with a foil covered neoprene stopper. All mixtures appeared clear during the stirring period.
After stirring, WAFs were removed through the outlet at the bottom of the vessel and added to test chambers. New WAF solutions were prepared for each 24-hour renewal.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: cultured in the Environmental Toxicology and Chemistry Laboratory of Exxon Biomedical Sciences, INC.
- Age of parental stock: 16 days
- Feeding during test
- Food type: Selenastrum capricornutum (algae)
- Amount: 2.32*10^5 cells/mL on days 0-6 and 1.56*10^5 cells/mL on days 7-21
- Frequency: daily
ACCLIMATION
- Acclimation period: no acclimation period (daphnia <= 24 hours old)
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES
Young were observed, counted and removed daily.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21
- Remarks on exposure duration:
- d
- Post exposure observation period:
- No post exposure period
Test conditions
- Hardness:
- No data
- Test temperature:
- Mean test temperature (water): 20°c (sd=0.05), continuously monitored by computer in test area.
- pH:
- 6.6 TO 7.8
- Dissolved oxygen:
- 5.2 TO 9.3 mg/L
- Salinity:
- Freshwater study
- Nominal and measured concentrations:
- Range finding test: Nominal concentrations (loading rates): 50, 10, 2.5, 1 and 0.5 mg/L. Samples of the control and the 50 mg/L loading were analyzed for the test substance by gas chromatography with flame ionization detection (GC-FID). The results of the analysis showed that the levels of the test susbtance were below the practical quantitation limit of 0.2 mg/L.
Definitive test: 0, 1, and 5 mg/L (nominal concentrations = loading rates) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: closed (flasks stoppered with foil covered neoprene stoppers on days 0-6; flasks covered with plexiglass sheets on days 7-21)
- Material, size, headspace, fill volume: 500 mL size glass erlenmeyer flasks, containing approximately 560mL of solution on days -6 (no head space) and 500 mL solution on days 7-21; test chambers rinsed with reverse osmosis water after renewals to remove food substrates
- Aeration: no aeration
- Renewal rate of test solution: renewal of the test solutions was performed on Monday, Wednesday and Friday by transferring the adult daphnids to new solutions via pipette.
- No. of organisms per vessel: range-finding test: 5; definitve test: 10
- No. of vessels per concentration (replicates): range-finding test: 2 (two trials); definitive test: 4
- No. of vessels per control (replicates): range-finding test: 2 (two trials); definitive test: 4
- Biomass loading rate: definitive test: 1 daphnid per approximately 50 to 56 mL of solution
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: BW3 supplemented with vitamin B12 and selenium
- Culture medium different from test medium: no
- Intervals of water quality measurement: The quality of the dilution water used in culture and testing is monitored at weekly, monthly, semi-annual and annual intervals.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h ightL/ 8 h Dark with gradual intensity conversion between periods
- Light intensity: approximately 630 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Adult mortality / immobilization
- Percent reduction in reproduction (based on cumulative young per adults)
RANGE-FINDING STUDY
- Test concentrations: 0, 0.5, 1.0, 2.5, 10, and 50 mg/L
- Loadings used for the definitive study: 0, 1, and 5 mg/L - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- EL50
- Effect conc.:
- > 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (loading rate)
- Basis for effect:
- reproduction
- Remarks:
- cumulative average young per adult
- Duration:
- 21 d
- Dose descriptor:
- EL50
- Effect conc.:
- > 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (loading rate)
- Basis for effect:
- immobilisation
- Remarks on result:
- other: (adults)
- Duration:
- 21 d
- Dose descriptor:
- NOELR
- Effect conc.:
- 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (loading rate)
- Basis for effect:
- reproduction
- Remarks:
- cumulative average young per adult
- Duration:
- 21 d
- Dose descriptor:
- NOELR
- Effect conc.:
- 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (loading rate)
- Basis for effect:
- immobilisation
- Remarks on result:
- other: (adults)
- Details on results:
- The first brood were observed in the control and each loading after 7 days. By day 14 a cumulative average of 20 young per female were produced in the control. A cumulative average of 54 young per female were produced in the control by day 21.
Other details on results in §Remarks on results including tables and figures. - Results with reference substance (positive control):
- No reference susbtance
- Reported statistics and error estimates:
- The No Observed Effect Loading Rate (NOELR) was determined using Dunnett's procedure of SAS.
Any other information on results incl. tables
Table 1. Definitive test: Percent adult mortality / immobilization and percent reduction in reproduction (based on cumulative youngs per adult) for the 14- and 21-day period.
Loading rate (mg/L) |
% Adult Mortality / Immobilization |
% Reduction in reproduction |
||
14 days |
21 days |
14 days |
21 days |
|
Control |
7.5 |
17.5 |
N/A |
N/A |
1.0 |
10 |
12 |
0 |
9 |
5.0 |
10 |
25 |
25 |
28 |
N/A - Not applicable, % reduction in reproduction as compared to the controls.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Dissolved oxygen fell below 60% sat. on day 12 in old solution. Since treatment was renewed, not believed to have effect on study. At day 21, 54 young per adult rather than 60, but believed sufficient young were produced for evaluation.
- Conclusions:
- The 21-day EL50s based on adult immobilization and reduction in reproduction were >5 mg/L. The 21-day No Observed Effect Loading Rates (NOELRs) based on adult immobilization and reduction in reproduction was 5 mg/L.
- Executive summary:
The 21-day EL50s based on adult immobilization and reduction in reproduction were >5 mg/L loading. The 21-day No Observed Effect Loading Rates (NOELRs) based on adult immobilization and reduction in reproduction was 5 mg/L.
The study was performed to evaluate the toxicity of the water accomodated fraction (WAF) of the test substance to the daphnid, Daphnia magna, in a semi-static system for a 21-day period. The loading rates for this test were 1 mg/L and 5 mg/L. A control of laboratory dilution water was also tested. Individual WAFs were prepared by adding the appropriate amount of the test substance to 3.5 liters of BW3 in a 4 L size glass aspirator bottle. The mixtures were then stirred for approximately 24 hours on a magnetic stirplate with a Teflon coated stirbar. Stirring was initiated using a vortex of <10% of the static liquid depth (1.5 cm). Each vessel was stoppered to prevent loss of volatiles. After stirring, the WAF was removed from the outlet at the bottom of the vessel.
Four replicates chambers were prepared per treatment by completely filling the test chambers with WAF (no headspace). Samples were removed from each WAF and the control on day 0, 2, 5, 7, 14, and at termination (composite of the replicate test chambers) for chemical analysis by gas chromatography with flame ionization detection (GS-FID). The results of the analysis showed that the levels of the test substance were below the practical quantitation limit of 0.2 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.