Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-926-1 | CAS number: 227605-94-9
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
not ready biodegradable
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
In the Klimisch 1 study from Grade (2001) the ready biodegradability of CA 3105 A (purity 93.9%) was determined according to OECD 301 B (CO2 evolution test). A deviation (one instead of 2 CO2 scrubbers) was addressed already in the report and was considered minor and had no impact on the outcome of the study.
The study was performed using sludge from the sewage treatment plant CH-4153 Reinach.
Following flasks (2 L brown glass, filled with 1.5 L test solution) were set up:
Flasks 1 and 2: test medium and inoculum (inoculum blank)
Flasks 3 and 4: reference substance and inoculum (procedure control)
Flasks 5 and 6: test item and inoculum
Flask 7: reference item, test item and inoculum (control of toxicity of test item)
Flask 8: distilled water.
The final test item concentration was about 50 mg/L.
Aeration was performed with 25 m/L CO2-free air. The CO2-scrubbers (250 mL glass vessels filled with 0.05N NaOH) were sampled after 3, 6, 8, 10, 13, 16, 20, 24, 28, 29 days. The last measurement was performed after acidification of the test medium with 1 mL concentrated hydrochloric acid. The test was performed at 22 +/-2°C. The CO2 evolution was determined as inorganic CO2 with a carbon analyser (Shimadzu TOC-5050 A)
After 29 days, the cumulative CO2 evolution in the reference substance flasks (sodium benzoate, 15 mg DOC/L) correspond to a degradation of 79% indicating correct performance of the test procedures. The cumulative CO2 evolution in the reference substance plus test item (toxic control) flasks correspond to a degradation of 85% of the positive control substance sodium benzoate, indicating that the test item had no toxic effect on the organisms at the tested concentration.
After 29 days, the cumulative CO2 evolution in the test item flasks correspond to a degradation of -1%. Negative degradation values were the consequence of the comparison of values obtained in the blanks and the low values obtained in the presence of the test item.
It can be concluded that the test item was not readily biodegradable under test conditions.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
