1,4,7-trioxacyclotridecane-8,13-dione

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999-12-07 to 1999-12-09

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study, GLP, no analytical dose verification

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,3,7,8-tetrahydro-2,5,8-benzotrioxacycloundecine-1,9-dione

EC Number:

229-554-3

EC Name:

2,3,7,8-tetrahydro-2,5,8-benzotrioxacycloundecine-1,9-dione

Cas Number:

6607-34-7

Molecular formula:

C10H16O5

IUPAC Name:

1,4,7-Trioxacyclotridecane-8,13-dione

Details on test material:

- Name of test material (as cited in study report): Adipic acid, Cyclicester with diethylenglycol
- Analytical purity: > 90%
- Impurities (identity and concentrations): Diethylenglycol <10% ; water<2%
- Purity test date: Jan 1999
- Lot/batch No.: B47

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 24 h
- Age of stock animals: 2-4 weeks

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.38 mmol/l

Test temperature:

20.5 - 20.6°C

pH:

8.0 - 8.2 at test start; 8.1 - 8.2 at test end

Dissolved oxygen:

8.6 - 8.7 at test start; 8.3 - 8.4 at test end

Nominal and measured concentrations:

0, 12.5, 25, 50, 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Flat bottom glass tubes, nominal volume 20 ml
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium
- Ca/mg ratio: 4:1
- Conductivity: 600 µSiemens/cm

OTHER TEST CONDITIONS
- Photoperiod: 16:8
- Light intensity: About 2 - 7 µE/(m2*s) at a wave length of 400 - 700 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Swimming ability after 0, 24 and 48 h

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

None of the daphnids were immobilized by the treatment, even at the highest concentration tested.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

• In the control the immobilization was < 10% . • In the control no daphnids were captured in a surface film. • The 02-concentration was > 3 mg/I in all tested replicates . • The pH-value in the test solutions should not vary within more than one unit .