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EC number: 204-070-5 | CAS number: 115-19-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 1986
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Life Science Research Ltd.
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-methylbut-3-yn-2-ol
- EC Number:
- 204-070-5
- EC Name:
- 2-methylbut-3-yn-2-ol
- Cas Number:
- 115-19-5
- Molecular formula:
- C5H8O
- IUPAC Name:
- 2-methylbut-3-yn-2-ol
- Details on test material:
- - Name of test material (as cited in study report): P0073
Constituent 1
Method
- Target gene:
- The characteristics of the individual strains are as follows:
TA 1535 - contains a histidine missense mutation but is also deficient in a DNA repair system (uvr B) and has a defective lipopolysaccharide coat on the cell wall. It is reverted by many agents causing base-pair substitutions, but is not sensitive to frameshift mutagens.
TA 1537 - bears a histidine frameshift mutation. Like TA 1535, it is defective in a DNA repair system and lipopolysaccharide coat. It is sensitive to agents causing frameshift mutations involving insertion or deletion of a single base-pair.
TA 1538 - contains another histidine frameshift mutation. Again it has a defective DNA repair system and lipopolysaccharide coat. It is reverted by agents causing deletion of two adjacent base-pairs (double frameshift mutations).
TA 100 - is the same as TA 1535 but contains a resistance transfer factor conferring ampicillin resistance and increasing sensitivity to some mutagens (plasmid pKM 101). In addition to base-pair substitutions, it is also able to detect certain frameshift mutagens.
TA 98 - is TA 1538 with the addition of the pKM 101 plasmid. It is reverted by a variety of mutagens, but not by simple alkylating agents causing base-pair substitutions.
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- - Type and identity of media: Cultures of all organisms were prepared by overnight incubation of freshly inoculated nutrient broth (Oxoid No.2).
- Species / strain / cell type:
- S. typhimurium TA 1538
- Details on mammalian cell type (if applicable):
- - Type and identity of media: Cultures of all organisms were prepared by overnight incubation of freshly inoculated nutrient broth (Oxoid No.2).
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver microsomal preparation (S9 mix)
- Test concentrations with justification for top dose:
- Series of eight different concentrations of test material from 2.5 ug to 5 mg per plate.
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: distilled water
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- 2 ug/plate TA1535
- Positive control substance:
- other: 2-acetylaminofluorene
- Remarks:
- 5 ug/plate TA 1535
- Positive control substance:
- 9-aminoacridine
- Remarks:
- 50 ug/plate TA 1537
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- 5 ug/plate TA 1538, TA 98
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- 5 ug/plate TA 1537, TA 1538, TA 100, TA 98
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Expression time (cells in growth medium): 48 hours
NUMBER OF REPLICATIONS: 3 on two separate occasions.
NUMBER OF CELLS EVALUATED:
DETERMINATION OF CYTOTOXICITY
- Method: thinning of the background lawn - Evaluation criteria:
- Increases in revertant colony numbers.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Slight reduction in background lawn
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Slight reduction in background lawn
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES: Preliminary toxicity test for dose selection using test strain TA 98.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
No increases in revertant colony numbers over control counts were obtained with any of the tester strains following exposure to test item at levels from 50 to 5000 ug per plate. Thus, the test item was considered non mutagenic under the conditions of the test. - Executive summary:
An in vitro study on bacteria was carried out according to EU method B.13/14 and OECD guideline 471. The test itemwas examined for mutagenic activity in five histidine-dependent auxotrophs of Salmonella typhimurium,strains TA 98, TA 1538, TA 100, TA 1535 and TA 1537, using pour-plate assays. Each test, in each strain, was conducted on two separate occasions and run in triplicate. The studies, which were conducted in the absence and presence of an activating system derived from rat liver (5-9 mix), employed a range of levels of test item from 50 to 5000 ug per plate, selected following a preliminary toxicity test in strain TA 98, and included solvent (distilled water) controls with and without S-9 mix. No increases in reversion to prototrophy were obtained with any of the five bacterial strains at the compound levels tested, either in the presence or absence of S-9 mix. Marked increases in the number of revertant colonies were induced by the known mutagens benzo[a]pyrene, 2-nitrofluorene, 2-aminoanthracene, 9-aminoacridine and sodium azide when examined under similar conditions. It was concluded that the test item was devoid of mutagenic activity under the conditions of the test.
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