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EC number: 688-011-2 | CAS number: 1243654-79-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22nd October 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies which did not affect the quality of relevant results.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.6800 (Modified Activated Sludge, Respiration Inhibition Test for Sparingly Soluble Chemicals)
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- GLP inspection was in 2008, and valid for 2 years.
Test material
- Reference substance name:
- Pellicer
- IUPAC Name:
- Pellicer
- Details on test material:
- Name: Pellicer
Lot No.: 01-09-17
Chemical name: L-Lysine, N2, N6-bis[N-(1-oxododecyl)-L-α (or γ)- glutamyl]-, sodium salt (1;?)
Purity: 91 %
Appearance: White powder
Storage: At room temperature in the dark, protected from humidity
Expiry date: 17 January 2011
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 162.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: 95% Confidence limits: 130.1 - 202.2 mg/L
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Any other information on results incl. tables
In comparison to the inoculum controls the respiration rate of the activated sludge was not inhibited in the concentration of 31 mg/L test item. The respiration rate was inhibited between 45.2 % and 83.3 % in the examined nominal test concentration range 100 – 1000 mg /L (95% Confidence limits: 130.1 - 202.2 mg/L). Concentrations exceeding 1000 mg/L nominal were not tested.
The 3-hour EC50 of 3,5-Dichlorophenol (positive control) was calculated to be 8.3 mg/L with 95 % confidence limits of 5.9 to 11.8 mg/L.
VALIDITY CRITERIA :
- The respiration rates of the two controls did not differ by more than 15 %
- The 3-hour EC50 of the reference item 3,5-Dichlorophenol for the used activated sludge batch was determined to be in the range of 5 to 30 mg/L.
- The concentration of dissolved oxygen did not drop below 2.5 mg O2/L during the incubation period, and just before the measurements of the respiration rates the oxygen concentrations were at least 6.5 mg O2/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- All validity criteria were met during this study.
- Conclusions:
- This study was carried out using OECD guidline 209 and EU method C11. The EC50 value was determined to be 162.2 mg/L (in the range of 100-1000 mg/L). The NOEC was determined to be < 10 mg/L.
- Executive summary:
A laboratory test was carried out with Pellicer to evaluate the effect of the test item on microorganisms by measuring the respiration rate. The test concentrations (10, 31, 100, 313 and 1000 mg/L) were chosen to permit the determination of the EC50.
In comparison to the inoculum controls the respiration rate of the activated sludge was inhibited between 4.8 % and 83.3 % in the examined nominal test concentration range. The inhibition showed a dose-related tendency. The respiration rate was inhibited with 83.3 % at the highest concentration level of 1000 mg/L. Concentrations exceeding 1000 mg/L nominal were not tested.
In parallel to the study with the test item, the reference item 3,5-Dichlorophenol was tested (the nominal test concentrations of 5, 16 and 32 mg/L) under otherwise identical test conditions.
The 3-hour EC50 of 3,5-Dichlorophenol was calculated to be 8.3 mg/L with 95 % confidence limits of 5.9 to 11.8 mg/L.
Based on measured inhibition rates the 3-hour EC50 value and the 95 %-confidence limits were calculated by Probit analysis using TOXSTAT software:
EC50: 162.2 mg/L (130.1 – 202.2 mg/L))
The EC50 value was determined to be 162.2 mg/L (in the range of 100-1000 mg/L).
The NOEC was determined to be < 10 mg/L.
The NOEC for respiration rates was not based on the results of a statistical analysis, but it is biologically justified. The respiration rates were inhibited and influenced dose dependently in the whole concentration range. The calculated respiration rates of the test item treated groups were not in the historical control data range (0.518 ± 0.074).
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