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EC number: 403-360-0 | CAS number: 42405-40-3 BONTRON E-84; E-84
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
In vitro
Bacterial cells in vitro:
The substance bis(3,5-bis(1,1-dimethylethyl)-2-hydroxybenzoato-O1,O2)zinc (CAS No. 42405-40-3) was tested in three in vitro gene mutation study in bacteria (Reverse Mutation Assay, Ames Test).
Two guideline assays found the substance was negative with and without metabolic activation. The assay chosen as the key study was performed in accordance with GLP and according to OECD 471 guidelines. Cytotoxicity was observed so dosing was adequate. None of the strains tested showed evidence of mutagenic activity, either in the absence or in presence of S9 mix.
Mammalian cells in vitro
Chromosome aberration
The substance bis(3,5-bis(1,1-dimethylethyl)-2-hydroxybenzoato-O1,O2)zinc (CAS No. 42405-40-3) was tested in four in vitro chromosome abberration assays in mammalian cells (in vitro mamamalian cytogenicity assay). In the key study the substance was tested in CHO cells with and without S9. There were no treatment-realted significant increases in structural chromosomal aberrations were seen following 21 hour non-activated treatments or 4 hour treatments with S-9. The substance is therefore considered to be non-clastogenic to CHO cells in vitro.
In vivo
In one guideline in vivo micronucleus assay, bis(3,5-bis(1,1-dimethylethyl)-2-hydroxybenzoato-O1,O2)zinc (CAS No. 42405-40-3) was tested at 1000 mg/kg in 5 male and female animals at 24h, 48h and 72h. There was no significant decrease in the ratio of poly-to normochromatic erythorcytes at any of the three sacrifice times after treatment with the test substance. Therefore, the substance was considered to be negative in an in vivo micronucleus assay .
Justification for selection of genetic toxicity endpoint
OECD Guideline and GLP compliant study
Short description of key information:
In vitro:
Gene Mutation (Bacterial Reverse Mutation Assay/Ames test): bis(3,5-bis(1,1-dimethylethyl)-2-hydroxybenzoato-O1,O2)zinc (CAS No. 42405-40-3) did not show mutagenic activity using S. typhimurium TA 1535, TA 1537, TA 98, TA 100 in the presence or absence of metabolic activation.
Chromosome aberration (Mammalian in vitro cytogenicity): bis(3,5-bis(1,1-dimethylethyl)-2-hydroxybenzoato-O1,O2)zinc (CAS No. 42405-40-3) did not display clastogenic activity in this assay.
In vivo:
Chromosome aberration (in vivo micronucleus assay): bis(3,5-bis(1,1-dimethylethyl)-2-hydroxybenzoato-O1,O2)zinc (CAS No. 42405-40-3) was found to be negative in an in vivo micronucleus assay.
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
Based on the available information in the dossier, the substance bis(3,5-bis(1,1-dimethylethyl)-2-hydroxybenzoato-O1,O2)zinc (CAS No. 42405-40-3) does not need to be classified for genetic toxicity when the criteria outlined in Annex I of 1272/2008/EC and Annex VI of 67/548/EEC are applied.
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