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EC number: 201-549-0 | CAS number: 84-65-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Key study: Acute oral: Experimental results: EU Method B.1 tris (Acute Oral Toxicity - Acute Toxic Class Method). GLP study. LD50 > 2000 mg/kg bw
Key study: Acute dermal: Experimental results.
LD50 > 3000 mg/kg bw
Acute inhalation: Data waiving: In accordance with column 2 of REACH Annex VIII, the study does not need to be conducted since exposure of humans via inhalation is not likely taking into account the vapour pressure of the substance and/or the possibility of exposure to aerosols, particles or droplets of an inhalable size.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23.6.-8.7.2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study was carried out in accordance with internationally valid GLP principles.
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 tris (Acute Oral Toxicity - Acute Toxic Class Method)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- yes
- Species:
- rat
- Strain:
- Wistar
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: breeding farm VELAZ s.r.o., Koleč u Kladna, Czech Republic
- Age at study initiation: 8-10 weeks at the time application
- Housing: animal room with monitoring conditions – 3 animals of one sex in one plastic breeding cage
- Diet: ST 1 BERGMAN – standard pelleted diet ad libitum
- Fasting period before study: about 20 hours
- Water: drinking tap water ad libitum
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): room temperature 22 +/- 3°C, permanently monitored
- Humidity (%): relative humidity 30 – 70 %, permanently monitored
- Air changes (per hr): approximately 15 air changes per hour
- Photoperiod (hrs dark / hrs light): light period 12-hour light/12 hour dark
STUDY TIME SCHEDULE
Animal supply: 18. 6. 2009
Experimental part of study: 23. 6. - 8. 7. 2009 - Route of administration:
- oral: gavage
- Vehicle:
- olive oil
- Details on oral exposure:
- VEHICLE
Batch No.: L803142
Expiration.: 09/2009
MAXIMUM DOSE VOLUME APPLIED: 2000 mg/kg body weight
DOSAGE PREPARATION: Immediately before application the test substance was weighed, mixed in vehicle (olive oil). The single volume of administered suspension was 1ml/100 g of animal body weight.
CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: Test procedure with a starting dose of 2000 mg/kg was selected. Testing schedule (according to EU Method B.1 tris Annex 1D) START: 2000 mg/kg – 3 females (Step No.1): no deaths ► 2000 mg/kg – 3 females (Step No. 2): no deaths ► END of study - Doses:
- 2000 mg/kg of animal body weight
- No. of animals per sex per dose:
- 6 animals (Step No.1 - 3 females, Step No. 2 - 3 females)
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Time schedule of observation:
Body weight: before application, on the 8th day of study and at day 15, before euthanasia of animals
Mortality: daily
Clinical examination: daily
Pathological examination: 15th day
- Necropsy of survivors performed: yes (gross necropsy) - Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Mortality:
- The test substance administered at the dose of 2000 mg/kg caused no death of animals.
- Clinical signs:
- other: No clinical signs of intoxication were detected after application in all animals.
- Gross pathology:
- The test substance caused no pathological changes in all animals from both groups.
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The test substance toxicity was evaluated on the basis of mortality, clinical signs of intoxication, body weight increments during the observation period and necropsy findings at the end of study.
The test substance administered at the dose of 2000 mg/kg caused no death of animals. No clinical signs of intoxication were observed in all animals. No pathologic macroscopic changes were diagnosed during pathological examination in all animals. - Executive summary:
The aim of the study was to investigate acute toxic effects of the test substance, Anthraquinone, after a single oral administration to Wistar rats.
The testing was performed according to the Method B.1 tris: Acute Oral Toxicity - Acute Toxic Class Method, Council Regulation (EC) No.440/2008, published in O.J. L 142, 2008.
The test substance was administered in a single dose as solution in vehicle (olive oil), given orally via gavage to two groups of three female Wistar rats.
The dosing was performed sequentially in two groups of three females: group No. 1 - first step using the starting dose of 2000 mg/kg of body weight and group No.2 - second step using the same dose.
The test substance administered at the dose of 2000 mg/kg caused no death of animals. No clinical signs of intoxication were observed in all animals.
No pathologic macroscopic changes were diagnosed during pathological examination in all animals
According to the study results the value of LD50 of the test substance for female rats is higher than 2000 mg/kg of body weight.
The classification of the test substance toxicity was performed according to the Directive 67/548/EEC, Annex VI. part 3.1.5. and 3.2.
Based on the test results and according to the EC criteria for classification and labelling requirements for dangerous substances and preparations the test substance Anthraquinone did not fall into any of quoted categories of toxicity and has no obligatory labelling requirement in this respect.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- discriminating dose
- Value:
- 2 000 mg/kg bw
- Quality of whole database:
- Klimisch 1. This study was carried out in accordance with internationally valid GLP principles.
Acute toxicity: via inhalation route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1974-1975
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Only brief summary available
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- 14 days study
- GLP compliance:
- not specified
- Test type:
- fixed dose procedure
- Limit test:
- no
- Species:
- rabbit
- Strain:
- other: New Zealand
- Sex:
- female
- Type of coverage:
- not specified
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- Applied in the form of an aqueous slurry - the test material could not be completely removed from the skin with tap water or other innocuous solvents.
- Doses:
- 300, 1000, 3000 mg/kg
- No. of animals per sex per dose:
- 1
- Control animals:
- not specified
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 3 000 mg/kg bw
- Based on:
- not specified
- Mortality:
- no
- Clinical signs:
- other: No pharmacotoxic symptoms were noted
- Gross pathology:
- Necropsy examination did not reveal any gross pathologic alterations.
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- LD50 > 3000 mg/kg.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- discriminating dose
- Value:
- 3 000 mg/kg bw
- Quality of whole database:
- Klimisch 2.
Additional information
Key study: Acute oral: Experimental results: EU Method B.1 tris (Acute Oral Toxicity - Acute Toxic Class Method). GLP study.
LD50 > 2000 mg/kg bw
Key study: Acute dermal: Experimental results.
LD50 > 3000 mg/kg bw
Acute inhalation: Data waiving: In accordance with column 2 of REACH Annex VIII, the study does not need to be conducted since exposure of humans via inhalation is not likely taking into account the vapour pressure of the substance and/or the possibility of exposure to aerosols, particles or droplets of an inhalable size.
Justification for selection of acute toxicity – oral endpoint
Only one study available.
Justification for selection of acute toxicity – inhalation endpoint
In accordance with column 2 of REACH Annex VIII, the study does not need to be conducted since exposure of humans via inhalation is not likely taking into account the vapour pressure of the substance and/or the possibility of exposure to aerosols, particles or droplets of an inhalable size.
Justification for selection of acute toxicity – dermal endpoint
Only one study available.
Justification for classification or non-classification
Based on the available data, the substance is not classified for acute toxicity:
LD50 oral > 2000 mg/kg bw
LD50 dermal > 3000 mg/kg bw
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