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EC number: 204-485-1 | CAS number: 121-60-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from an authoritative database.
Data source
Reference
- Reference Type:
- other: Authoritative database
- Title:
- Bacterial reverse mutation study (BRM) using the test chemical
- Author:
- J-Check
- Year:
- 2 001
- Bibliographic source:
- J-Check_121608
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- According to OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- N-acetylsulphanilyl chloride
- EC Number:
- 204-485-1
- EC Name:
- N-acetylsulphanilyl chloride
- Cas Number:
- 121-60-8
- Molecular formula:
- C8H8ClNO3S
- IUPAC Name:
- 4-acetamidobenzenesulfonyl chloride
- Test material form:
- solid: crystalline
- Details on test material:
- - Name of test material (as cited in study report): N-acetylsulphanilyl chloride
- Substance type: Organic
- Physical state: solid
Constituent 1
- Specific details on test material used for the study:
- Purity:99.7%
Method
- Target gene:
- His and Trp
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver S9 microsomal fraction was prepared from phenobarbital and 5,6-bezoflavone-induced male Sprague Dawley rats.
- Test concentrations with justification for top dose:
- i) Plate Incorporation Method:
Without S9: 0, 156.3, 312.5, 625, 1250, 2500 and 5000 ug/plate in the strains of TA 100, TA 1535 and E.coli WP2.
: 0, 31.3, 62.5, 125, 250, 500 and 1000 ug/plate in the strains of TA 98 and TA 1537
With S9: 0, 156.3, 312.5, 625, 1250, 2500 and 5000 ug/plate (For all strains)
ii) Pre-incubation Method
Without S9: 0, 78.1, 156.3, 312.5, 625, 1250, 2500 and 5000 ug/plate in the strains of TA 100, TA 1535 and E.coli WP2.
: 0, 31.3, 62.5, 125, 250, 500 and 1000 ug/plate in the strains of TA 98 and TA 1537
With S9: 0, 78.1, 156.3, 312.5, 625, 1250, 2500 and 5000 ug/plate (For all strains) - Vehicle / solvent:
- N,N-dimethylformamide
- Justification for choice of solvent/vehicle: The test chemical was soluble in N,N-dimethylformamide
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- N,N-dimethylformamide
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- furylfuramide
- other: 2- aminoanthracene
- Details on test system and experimental conditions:
- NUMBER OF REPLICATIONS:
- Number of cultures per concentration (single, duplicate, triplicate) : Triplicate per test
- Number of independent experiments : Two
METHOD OF TREATMENT/ EXPOSURE:
- Cell density at seeding (if applicable): NA
- Test substance added in medium; in agar (plate incorporation)
- Rationale for test conditions:
- A confirmatory test was carried out in pre-incubation method, as negative mutagenic effects was observed in plate incorporation method.
- Evaluation criteria:
- The test chemical was adjudged as a postive mutagen when the number of the revertant colonies in the test chemical treated increased dose dependently and became two-fold or more than that of the negative control and significant reproducible increase was noted at one or more concentration and at least in one strain with or without metabolic activation system and is considered as negative when any of the above criteria was not fulfilled.
- Statistics:
- No statistical analysis was performed.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- At 1000 ug/plate concentration without S9, above 2500 ug/plate with S9.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- At and above 2500 ug/ml concentration with and without S9.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- At 1000 ug/plate concentration without S9 and at 2500 ug/plate with S9.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- At 1000 ug/plate concentration without S9, at 2500 ug/plate with S9.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- At 2500 ug/ml concentration with and without S9.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: No mutagenic potential of the test chemical was observed.
Applicant's summary and conclusion
- Conclusions:
- The registered chemical, 4-Acetamidobenzenesulfonyl chloride (CAS 120-60-8) was tested negative in bacterial reverse mutation test (according to OECD TG 471) in S. typhimurium TA 98, TA100, TA 1535 and TA 1537 and E. coli WP2 uvrA strains, both in the presence and absence of S9 metabolic activation system.
- Executive summary:
A GLP-compliant bacterial reverse mutation study (OECD TG 471) was performed to assess the mutagenic potential of the registered substance in S. typhimurium and E.coli strains. The following strains were used: Salmonella typhimurium TA 98, TA100, TA 1535 and TA 1537 and E. coli WP2 uvrA.
The following test concentrations were used:
i) Plate Incorporation Method:
Without S9: 0, 156.3, 312.5, 625, 1250, 2500 and 5000 ug/plate in the strains of TA 100, TA 1535 and E.coli WP2.
: 0, 31.3, 62.5, 125, 250, 500 and 1000 ug/plate in the strains of TA 98 and TA 1537
With S9: 0, 156.3, 312.5, 625, 1250, 2500 and 5000 ug/plate (For all strains)
ii) Pre-incubation Method
Without S9: 0, 78.1, 156.3, 312.5, 625, 1250, 2500 and 5000 ug/plate in the strains of TA 100, TA 1535 and E.coli WP2.
: 0, 31.3, 62.5, 125, 250, 500 and 1000 ug/plate in the strains of TA 98 and TA 1537
With S9: 0, 78.1, 156.3, 312.5, 625, 1250, 2500 and 5000 ug/plate (For all strains)
N,N-dimethylformamide was used as a vehicle. A confirmatory test according to the pre-incubation method was carried out, as the test chemical was found non-mutagenic in the first experiment performed according to the plate incorporation method. The test chemical was considered positive (mutagenic) when the number of the revertant colonies in treated cultures increased dose-dependently and became two-fold or more than that of the vehicle negative control, and a significant reproducible increase was noted at one or more concentration and at least in one strain with or without metabolic activation system. The test chemical was considered negative (non-mutagenic) when any of the above criteria were not fulfilled. No statistical analysis was performed. Results: The test substance did not induce a two-fold and/or biologically relevant increase in revertant colony counts compared with the vehicle control when it was tested up to 5000µg/plate neither in the presence nor the absence of S9 metabolic activation in S. typhimurium and E. coli strains used. Conclusion:The test substance was considered non-mutagenic (negative) in the bacterial reverse mutation test (OECD TG 471).
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