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EC number: 276-657-4 | CAS number: 72428-99-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014-2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well performed OECD study with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Hydrogen [[[(2-ethylhexyl)amino]sulphonyl][[(3-methoxypropyl)amino]sulphonyl]-29H,31H-phthalocyaninesulphonato(3-)-N29,N30,N31,N32]cuprate(1-), compound with N,N'-di(o-tolyl)guanidine (1:1)
- EC Number:
- 276-657-4
- EC Name:
- Hydrogen [[[(2-ethylhexyl)amino]sulphonyl][[(3-methoxypropyl)amino]sulphonyl]-29H,31H-phthalocyaninesulphonato(3-)-N29,N30,N31,N32]cuprate(1-), compound with N,N'-di(o-tolyl)guanidine (1:1)
- Cas Number:
- 72428-99-0
- Molecular formula:
- C32H13CuN8O4S2.(SO3H)m.(C4H11NO)n.(C8H18N) (3-m-n).C15H17N3
- IUPAC Name:
- hydrogen [[[(2-ethylhexyl)amino]sulphonyl][[(3-methoxypropyl)amino]sulphonyl]-29H,31H-phthalocyaninesulphonato(3-)-N29,N30,N31,N32]cuprate(1-), compound with N,N'-di(o-tolyl)guanidine (1:1)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: The saturated solution* was tested in a limit test.
- Sampling method: The concentrations of the test item were analytically determined from the limit concentration and the control at the start of the exposure (0 h) and at the end of the exposure (72 h). Samples at the start of the exposure were taken from an additional replicate without algae. At the end of the exposure samples were taken directly from the pooled test replicates.
- Sample storage: The original and diluted samples were stored at room temperature until sample preparation and until analysis, if necessary.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution (5 mg/L test item was weighed out) was freshly prepared with dilution water. The stock solution was stirred for 48 hours (1100 rpm) with a magnetic stirrer. Undissolved particles were removed by centrifugation for 10 min at 18533 g. The saturated solution was checked for the presence of dispersed test item (by Tyndall effect via Laser beam).
- Eluate: Dilution water
- Differential loading: None, limit test with the saturated solution (initially measured concentration of 1.11 mg/L)
- Controls: Six replicates (without test item) were tested under the same test conditions as the test vessels.
- pH-Controls: None
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus Chodat
- Strain: SAG 86.81
- Source (laboratory, culture collection): SAG Pflanzenphysiologisches Institut der Universitaet Goettingen, Nikolausberger Weg 18, D-37073 Goettingen, Germany
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: Fresh stocks were prepared every month on Z-Agar. Light intensity amounted to 35 - 70 µE x m-2 x s-1 for 24 h per day.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No
Test conditions
- Hardness:
- not specified
- Test temperature:
- min.: 22.0 max.: 23.0, mean value: 22.5 °C
- pH:
- Initial mean measured pH-value
test item concentration
[mg/L] Start; 0 hours End; 72 hours
1.11 7.56 8.28
Control 8.00 8.49 - Dissolved oxygen:
- Not measured
- Salinity:
- Not measured, freshwater conditions
- Nominal and measured concentrations:
- See window "Any other information on materials and methods incl. tables"
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): open, cotton wool plugs
- Material, size, headspace, fill volume: sterile 250 mL Erlenmeyer flasks, test volume 25 mL
- Aeration: Test containers were placed on a rotary shaker and oscillated at appr. 70 rpm
- Type of flow-through (e.g. peristaltic or proportional diluter): None
- Renewal rate of test solution (frequency/flow rate): One application at test start
- Initial cells density: Nominal: approximately 2 - 5 x 10^3 cells/mL, Actual: mean 4998 cells/mL
- Control end cells density: Mean 920802 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Composition of Dilution Water
according to the guideline
Component Concentration [mg/L]
NH4Cl 15
MgCl2 x 6 H2O 12
CaCl2 x 2 H2O 18
MgSO4 x 7 H2O 15
KH2PO4 1.6
FeCl3 x 6 H2O 0.064
Na2EDTA x 2 H2O 0.1
H3BO3 0.185
MnCl2 x 4 H2O 0.415
ZnCl2 3 x 10^-3
Na2MoO4 x 2 H2O 7 x 10^-3
CoCl2 x 6 H2O 1.5 x 10^-3
CuCl2 x 2 H2O 1 x 10^-5
NaHCO3 50
pH 8.1 ± 0.2
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: 24 h
- Light intensity and quality: 4440 - 8880 lux, corresponding to 60 - 120 µE x m^-2 x s^-1, mean value: 7030 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] fluorimeter, Cell density and self fluorescence was measured daily via Chlorophyll-a- fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as background signal. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.11 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1.11 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: No
- Colour differences: No
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Other: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
Current Study Valid Range (average ± 3 x SD)
Growth Rate Inhibition
ErC50 0.684 0.684 ± 0.281
95 % confidence interval 0.657 - 0.714 0.684 ± 0.281
Yield Inhibition
EyC50 0.315 0.314 ± 0.087
95 % confidence interval 0.297 - 0.3355 0.314 ± 0.087
SD = Standard deviation - Reported statistics and error estimates:
- EC-values of the growth rate and yield inhibition were estimated empirically based on the results of the only treatment level (Limit test design).
NOEC/LOEC were determined by calculation of statistical significance of growth rate and yield. The t-test was used for NOEC/LOEC calculations. When running a t-test a Normality test and an Equal Variance test were done first. The SHAPIRO-WILK-Test was used to test for normally distributed populations. The Levene median test was used for equal variance. P-values for both Normality and Equal Variance tests are 0.05. The -value (acceptable probability of incorrectly concluding that there is a difference) is =0.05. The Equal Variance Test failed for growth rate and yield data. No transformation was found to pass the Equal Variance Test.
Any other information on results incl. tables
Cell Densities
Initial measured test item concentration |
Replicate |
Cell density [cells/mL] |
|||
[mg/L] |
No. |
0 hours |
24 hours |
48 hours |
72 hours |
1.11 |
1 |
4998 |
28514 |
169673 |
926014 |
2 |
4998 |
37004 |
193193 |
927573 |
|
3 |
4998 |
24334 |
169309 |
955944 |
|
4 |
4998 |
25101 |
170141 |
942560 |
|
5 |
4998 |
25746 |
163912 |
875292 |
|
6 |
4998 |
27973 |
185431 |
1023473 |
|
Mean |
4998 |
28112 |
175277 |
941809 |
|
Control |
1 |
4998 |
25577 |
162665 |
1000256 |
2 |
4998 |
27007 |
163410 |
849779 |
|
3 |
4998 |
22229 |
166217 |
821625 |
|
4 |
4998 |
27617 |
159208 |
1026721 |
|
5 |
4998 |
29042 |
177600 |
992372 |
|
6 |
4998 |
23321 |
153742 |
834056 |
|
Mean |
4998 |
25799 |
163807 |
920802 |
Evaluation after 72 hours
Statistically significant differences of growth rates and yield compared to
control values are marked (+), not significant differences are marked (-).
Initial measured test item concentration |
Replicate |
Growth rate |
Rate-related inhibition |
Yield |
Inhibition of yield |
||
[mg/L] |
No. |
[d-1] |
[%] |
[cells/mL] |
[%] |
||
1.11 |
1 |
|
1.74 |
0 |
|
921016 |
-1 |
2 |
|
1.74 |
0 |
|
922575 |
-1 |
|
3 |
|
1.75 |
-1 |
|
950946 |
-4 |
|
4 |
|
1.75 |
-1 |
|
937562 |
-2 |
|
5 |
|
1.72 |
1 |
|
870294 |
5 |
|
6 |
|
1.77 |
-2 |
|
1018475 |
-11 |
|
Mean |
(-) |
1.75 |
-1 |
(-) |
936811 |
-2 |
|
Control |
1 |
|
1.77 |
|
|
995258 |
|
2 |
|
1.71 |
|
|
844781 |
|
|
3 |
|
1.70 |
|
|
816627 |
|
|
4 |
|
1.78 |
|
|
1021723 |
|
|
5 |
|
1.76 |
|
|
987374 |
|
|
6 |
|
1.71 |
|
|
829058 |
|
|
Mean |
|
1.74 |
|
|
915804 |
|
Section-by-Section and Average Specific Growth Rates of the Control Group (0 – 72 hours)
|
Replicate No. |
Specific growth rate [d-1] |
Mean (0 - 72 h) |
SD ± |
CV |
Mean CV [%] |
||
section-by-section |
||||||||
0 - 24 hours |
24 - 48 hours |
48 - 72 hours |
||||||
Control |
1 |
1.63 |
1.85 |
1.82 |
1.77 |
0.117 |
6.62 |
7.43 |
2 |
1.69 |
1.80 |
1.65 |
1.71 |
0.079 |
4.60 |
||
3 |
1.49 |
2.01 |
1.60 |
1.70 |
0.275 |
16.1 |
||
4 |
1.71 |
1.75 |
1.86 |
1.78 |
0.080 |
4.50 |
||
5 |
1.76 |
1.81 |
1.72 |
1.76 |
0.045 |
2.57 |
||
6 |
1.54 |
1.89 |
1.69 |
1.71 |
0.173 |
10.2 |
||
|
|
|
Mean |
1.74 |
|
|
||
|
|
|
SD ± |
0.03 |
|
|||
|
|
|
CV [%] |
1.98 |
|
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study the test item was found not to inhibit the growth of the freshwater green alga Desmodesmus subspicatus after 72 hours. The NOEC-value of the test item for both inhibition of growth rate and inhibition of yield after 72 hours was 1.11 mg/L. The EC50-values of the test item for both inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were > 1.11 mg/L.
All effect levels are given based on initially measured concentration of Savinyl Blue GLS. - Executive summary:
The toxicity of the test item to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201 (2011) .The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours.
The study was conducted under static conditions with an initial cell density of 4998 cells/mL. A saturated solution* with a nominal concentration of 5 mg/ Lwas tested.Six replicates were tested for the limit concentration and the control. Environmental conditionswere determined to be within the acceptable limits.
The concentrations of the test item were analytically verified via HPLC-DAD at the start and the end of the exposure of the limit test item concentration and the control. The recovery rate at the end of the exposure was 94 % of the initial measured limit test item concentration. Therefore, all effect values given are based on the initial measured test item concentration of the test item
NOEC, LOEC and EC - values (0-72 hours)
based on initial measured test item concentrations [mg/L]
Growth Rate Inhibition
Initial measured test item concentration [mg/L]NOEC
1.11
LOEC
> 1.11
ErC10
> 1.11
ErC20
> 1.11
ErC50
> 1.11
Inhibition of Yield
Initial measured test item concentration [mg/L]
NOEC
1.11
LOEC
> 1.11
EyC10
> 1.11
EyC20
> 1.11
EyC50
> 1.11
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