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EC number: 956-606-9 | CAS number: 1373821-23-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
QSAR model, iSafeRat , key study, validity 1:
48h-EC50 = 6.4 mg/L (95% CL: 5.7 - 7.1 mg/L).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 6.4 mg/L
Additional information
To assess the short-term toxicity of the registered substance to aquatic invertebrates, two data are available.
The first data (QSAR-KREATiS, 2021) is assessed as the key study and is a QSAR. This QSAR prediction (iSafeRat daphnEC50 ) was performed on the registered substance, to assess the acute toxicity of the substance to daphnids. This QSAR has been validated to be compliant with the OECD recommendations for QSAR modelling (OECD, 2004) and predicts the endpoint value which would be expected when testing the substance under experimental conditions in a laboratory following OECD Guideline 202. The immobility of the daphnids was determined using a validated QSAR model for the Mechanism of Action (MechoA) in question (MechoA 1.1, i.e. non-polar narcosis) (Bauer et al., 2018). The QSAR model is based on validated data for a training set of 58 chemicals derived from 48-hour test on daphnids, for which the concentrations of the test item had been determined by chemical analyses over the test period. The test item falls within the applicability domain. The 48-h EC50 based on mobility was determined to be 6 mg/L with 95%-Confidence Limit between 5.7 and 7.1 mg/L.
The second data (phytosafe, 2012) is an experimental study assessed as not realiable . A Study was performed according to OECD Guideline 202 with GLP statement, to determine the treatment concentrations liable to immobilize 50 % of the daphnids (EC50) after 24 and 48 h exposure of the test item to Daphnia magna, under static conditions.A 48 h range-finding test was conducted and the percent immobilization was 0, 0, 20, 0, 100, 100 and 100 % at 0.01, 0.1, 1.0, 9.9 and 98.8 mg/L, respectively.
No immobilization was observed throughout the test period for the water and solvent control. The percentage of immobilization was 20 % as a maximum for the 0.01 and 0.1 mg/L test item treatments, at 1.0 mg/L 40 % of the daphnids were immobilized after 24 h of standing and then 100 % after 48 h of testing. At both 9.9 and 98.8 mg/L all the daphnids were immobilized after 24 h of testing. The measured concentrations of florenthene as percent nominal value were 109.8, 96.1, 94.7, 95.3 and 102.4 % at initial medium while 100.9, 97.3, 96.4, 95.7 and 98.6 % at final medium in definitive test. The test item treatments were satisfactorily maintained between 80-120 % of the nominal values throughout the test period.
Under the test conditions, 48h-EC50 (Daphnia magna) = 0,38 mg/L (95% CL: 0,12-1,20 mg/L) based on analytically confirmed nominal concentrations (recovery of the substance at the end of the test: 95.7-100.9 %).
However, this study is not reliable due to high concentration of solvent used. Indeed, a large quantity of acetone was used as solvent in this study. Because of the potential for interaction with the test chemical resulting in an altered response in the test, solvent use should be restricted to situations where no other acceptable method of test solution preparation is available. The use of solvent is not the best method at the time being. Then, the concentration/quantity of solvent used in the treatment solutions was 5 mL/L, corresponding to 3.95 g/L (with a density of 0.79), which is 50 times higher than the recommended maximum level of solvent (below 0.1 mL/L; OECD 23) and represent almost half of the EC50 of acetone (which was reported in the ECHA disseminated dossier at 8.8 g/L). Acetone is suspected to have affected the definitive results. Some solvent/substance interactions may have occurred in this study with this high concentration of acetone.
In conclusion, the experimental range-finding tests performed on the registered substance supports the key prediction data. Therefore, the 48h-EC50 value was considered to be 6,4 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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