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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019-12-20 - 2020-02-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Also according to GLP.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Version / remarks:
- July 17 1992
- Deviations:
- no
- GLP compliance:
- yes
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): sludge sampling was carried out at 10 locations (surface water and surface soil of rivers, lake, and inland sea, and return sludge from sewage plants). Sampled Nov 2019.
- Method of cultivation: Activated sludge was cultivated for 19.3h after feeding with the synthetic sewage.
- Storage length: 1 month
- Preparation of inoculum for exposure: amount of activate sludge was added to culture medium
- Concentration of sludge: 30 mg/L (as concentration of suspended solid) - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Based on the guideline
- Solubilising agent (type and concentration if used): not applicable
- Test temperature: 25 °C
- pH: 7.1-7.2
- pH adjusted: yes
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Glass vessel
- Number of culture flasks/concentration: 3 for the test item with sludge, 1 for the test item and water, 1 control
- Measuring equipment: closed system oxygen consumption measuring apparatus/GC
- Test performed in closed vessels due to significant volatility of test substance: no
SAMPLING
- Sampling frequency: Weekly
- Sampling method: Closed measurement analysis
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Reference substance:
- aniline
- Test performance:
- No adverse effects on the reliability of this test were noted.
- Parameter:
- % degradation (O2 consumption)
- Value:
- -3
- Sampling time:
- 28 d
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 1
- Sampling time:
- 28 d
- Details on results:
- Undissolved material observed in the test item vessels.
- Results with reference substance:
- 70% at Day 7
90% at Day 14 - Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test item did not undergo biodegradation under the test conditions of this study.
- Executive summary:
The biodegradability of the test item was investigated in an OECD TG 301C test which complied with GLP. A concentration of 100 mg/L of test item was used and incubated with 30 mg/L of activated sludge for 28 days at ca. 25 °C. The biodegradation of the test item was followed through measurement of the biochemical oxygen demand in order to determine oxygen consumption and through direct analysis of the test item by gas chromatography.
The average biodegradation of the test item measured through oxygen consumption was -3%.
The average biodegradation of the test item measured through GC analysis was 1%.
Therefore, it is concluded that the test item did not undergo biodegradation under the test conditions of this study.
Reference
Validity criteria:
- Difference between extremes of replicates of the test solutions: 1%
- Percentage degradation with the reference substance: 70% at day 7 and 90% at day 14
- BOD in control: 25 mg/L
Table. Percentage biodegradation by BOD
Day 7 | Day 14 | Day 21 | Day 28 | Average | ||||||
BOD | Deg (%) | BOD | Deg (%) | BOD | Deg (%) | BOD | Deg (%) | Deg (%) | ||
Water + test item | 0 | - | 0 | - | 0.3 | - | 0.5 | - | - | |
Sludge + test item | 1 | 1.9 | 0 | 3.5 | -2 | 4.5 | -2 | 5.1 | -3 | -3 |
2 | 2.1 | 0 | 3.8 | -2 | 4.8 | -2 | 5.5 | -2 | ||
3 | 2.1 | 0 | 3.7 | -2 | 4.6 | -2 | 5 | -3 | ||
Control | 1.8 | - | 5.4 | - | 6.7 | - | 7.6 | - | - | |
Reference substance | 52.6 | 70 | 70.8 | 90 | 75.1 | 95 | 77.1 | 96 | - |
Table. Percentage degradation by test item analysis at Day 28.
Amount test item (mg) | Percentage residual | Percentage degradation | ||
Water + test item | 29.1 | 97 | ||
Sludge + test item | 1 | 28.9 | 96 | 1 |
2 | 28.7 | 96 | 2 | |
3 | 28.6 | 95 | 2 |
Description of key information
The test item did not undergo biodegradation in an OECD 301C test.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
The biodegradability of the test item was investigated in an OECD TG 301C test which complied with GLP. A concentration of 100 mg/L of test item was used and incubated with 30 mg/L of activated sludge for 28 days at ca. 25 °C. The biodegradation of the test item was followed through measurement of the biochemical oxygen demand in order to determine oxygen consumption and through direct analysis of the test item by gas chromatography.
The average biodegradation of the test item measured through oxygen consumption was -3%.
The average biodegradation of the test item measured through GC analysis was 1%.
Therefore, it is concluded that the test item did not undergo biodegradation under the test conditions of this study.
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