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EC number: 607-988-8 | CAS number: 2668-75-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jan - Mar 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 17-Acetoxy-1,4,6-pregnatriene-3,20-dione
- EC Number:
- 607-988-8
- Cas Number:
- 2668-75-9
- Molecular formula:
- C23 H28 O4
- IUPAC Name:
- 17-Acetoxy-1,4,6-pregnatriene-3,20-dione
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
The activated sewage sludge for the inoculum was collected on the day before the start of the experiment from a well-operated municipal sewage treatment plant ("Kläranlage Berlin-Ruhleben"), predominantly dealing with domestic sewage. Upon arrival at the laboratory, the activated sludge was stirred and aerated tor approximately 3 hours. Thereafter, the sludge was homogenized in a blender. Then it was allowed to settle tor approximately 1 hour. 24 mL (90 mg) suspended solids were taken from the supernatant for each test vessel. - Duration of test (contact time):
- 36 d
Initial test substance concentration
- Initial conc.:
- 10 mg/L
- Based on:
- DOC
- Details on study design:
- TEST CONDITIONS
The test solutions were incubated at 20 to 24°C for 36 days (except on day 29 when the room temperature rose to 28 °C for a period of approximately 16 hours after wh ich the room temperature decreased to 17 -18 °C and increased to a normal temperature of 20-21 °C on day 32 due to technical problems with the air-conditioning system). Furthermore, the test solutions were supplied with filtered CO2-free aeration (2.0 to 7.5 L air per hour for each test vessel) for 36 days. On day 35 the pH of the test solutions, including reference and blank control, ranged from 7.6 to 8.0. After the measurements of pH on day 35,1 mL of concentrated HCL was added to each test vessel in order to convert all dissolved inorganic carbon into CO2.
TEST SYSTEM
For the determination of the CO2 produced, thme CO2 absorber bottles, filled with 100 ml 0.025 N Ba(OH)2 each, were connected in series to the exit air-pipe of each test bottle. The amount of CO2 produced was determined by titration of the remaining Ba(OH)2 with 0.05 N standardised HCI. On days 3, 4, 7, 9, 11, 14, 18, 23, 28, 30, 32, 35 and 36 the CO2 absorber bottle nearest to the test bottles was removed for the titration. The remaining two absorbers were each moved one place closer to the test vessel and a new absorber bottle filled with fresh Ba(OH)2 was placed at the far end of the series. In cases where BaCO3 was also precipitated in the second absorber bottle of any solution, titrations of two Ba(OH)2 bottles were performed. Subsequently, two fresh absorbers were added.
24 mL of the inoculum from the activated sludge were added to the nutrient solutions in each of the test vessels. These mixtures were aerated wiith CO2-free air for about 24 hours to purge the system of carbon dioxide before test and reference compounds were added. CO2-free air was obtained by bubbling filtered air through activated charcoal and soda lime pellets. A stock solution of the reference substance was prepared by dissolving 1.0 g sodium acetate in 1 L demineralized water. 102 mL of this stock solution were added to the reference test bottle and into the bottle for the toxicity controI. The test material was dispersed weil in vessels containing approximately 1 mL water and added directly at amounts of 40.0 mg ZK 5560 to each of three test bottles and one bottle for the toxicity control. Finally, the test vessels were rinsed with 2-3 mL demineralized water. Finally, each test vessel was filled up with deminelfalized water to a volume of 3000 mL. Thus, the concentrations (based of carbon) of the reference and the test compounds were as folIows:
ZK 5560: 10 mg/L
reference substance (sodium acetate): 10 mg/L
toxicity control (ZK 5560 + sodium acetate): 10 mg/L + 10 mg/L
Three test bottles contained only nutrient reagents and inoculum and served as blank (control) vessel.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 71
- Sampling time:
- 34 d
- Remarks on result:
- other: failed 10 day window
- Details on results:
- After a relatively long lag-phase (until day 18) the test compound was degraded rapidly to 71% on day 36 (34 days of incubation).
BOD5 / COD results
- Results with reference substance:
- The reference compound sodium acetate produced 99 mg CO2 which was equivalent to 90% of the theoretical CO2 production.
Any other information on results incl. tables
Table 1: Biological degradation (cumulative) in percent (corrected for blank CO2 production) of Delta-1,6-Hydroxyprogesteroncetat
Test | Nominal | Day of sampling |
|||||||||||
compound | concentration of carbon | 3 | 4 | 7 | 9 | 11 | 14 | 18 | 23 | 28 | 30 | 32 | 35 |
ZK 5560 | 10 mg/l | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 18 | 45 | 59 | 66 | 69 |
Sodium acetate (reference) | 10 mg/l | 15 | 33 | 58 | 68 | 73 | 80 | 84 | 87 | 89 | 88 | 88 | 89 |
ZK 5560 + | 10 mg/l + | 7 | 15 | 27 | 43 | 54 | 67 | 76 | 82 | 84 | 85 | 86 | 86 |
sodium acetate | 10 mg/l | ||||||||||||
(toxicity control) |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable, not fulfilling specific criteria
- Conclusions:
- The test compound Delta-1,6-Hydroxyprogesteronacetat is not readily biodegradable according to the OECD criteria but considered being biodegradable based on the high amount degraded at the end of the study.
- Executive summary:
The test substance Delta-1,6-Hydroxyprogesteronacetat was incubated in an aqueous solution including nutrients with microorganisms from a municipal sewage treatment plant for 34 days (start of treatment = day 1). The experiment was extended to 34 days because the plateau was not reached by day 28. The nutrient solutions were made up of phosphates, ammonium sulphate, magnesium sulphate, iron chloride, ammonium chloride and calcium chloride and added to the test solution. The test substance was incubated in a c:oncentration of 10 mg carbon/L in triplicate. Additionally, a reference substance (sodium acetate) was tested according to the same procedure, in order to verify the viability and activity of the degrading microorganisms. Furthermore, a blank control was tested in triplicate without any test or reference substance. One further set was incubated with sodium acetate as 10 mg carbon/L (reference substance) plus test substance as 10 mg carbon/L representing a toxicity control. The biological degradation of the test and reference substances was evaluated by measurement of the carbon dioxide (C02) produced during the test period. CO2 production was determined on days 3, 4, 7, 9, 11, 14, 18, 23, 28, 30, 32, 35 and 36 and calculated as the percentage of total CO2 that the test material could theoretically have produced, based on carbon content. The blank CO2 production was subtracted for correction. After a relatively long lag-phase (until day 18) the test compound Delta-1,6-Hydroxyprogesteronacetat was degraded rapidly to 71% on day 36 (~ 34 days of incubation).
The reference compound sodium acetate was degraded to 73% on day 11 (~ 10 days of incubation) and up to 90% on day 36.
In the toxicity control, the reference compound (sodium acetate) plus the test compound, was degraded to 87% on day 36 (~ 34. days of incubation). The degradation of the toxicity control seemed to start on days 9 to 11, earlier than in the individual set, indicated by the higher degradation in the toxicity control compared to the individual sets.
The test compound Delta-1,6-Hydroxyprogesteronacetat is not readily biodegradable based on the OECD criteria since the 60% trigger was not reached after 28 d of incubation. However, the substance is considered biodegradable to a high extent since 71% were degraded after approx. 34 d of incubation.
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