Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 953-553-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 31, 2022
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- The average log Kow varied from 5.4 to 6.0.
The water solubility was from 0.8 mg L-1 for the congener containing 4 Cl atoms to 0.3 mg L-1 for the congener with 9 Cl atoms. - Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- WAF preparation:
Loading rates (nominal concentrations): A stock elution was prepare using a nominal concentration of 1000 mg/L. After adding salts, it was then diluted to get all the tested concentrations.
Sample weight: Direct addition of 1000 mg (actial weight = 999.8 mg) per liter for the stock elution
Ambient temperature 20°C. Orbital shaker 100 rpm- 7 days.
At the end of shaking, the eluate is slightly turbid and whitish, partly jellified and hard to filter. After longer decantation a thin a whitish layer settles on the bottom of the container.
Filtration: With vacuum filtration apparatus and membranes with 0.45 µm pores of cellulose nitrate. After filtrations, the stock eluate was added with the salts and dilutions were prepared using the cultivation medium. Oxygenation of the eluate and its dilutions followed, via air insufflation for 30’ prior to starting the test and acclimatation to test temperature (20 °C). - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- The fishes came from a single group of similar length (1-2 cm) and age.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 160 mg CaCO3/L
- Test temperature:
- 23 °C
- pH:
- 7.5
- Dissolved oxygen:
- ≥ 60% (i.e. 5.14 mg/L at 23 °C and 1 atm)
- Salinity:
- 0.13 ‰
- Nominal and measured concentrations:
- Limit test at 1000 mg/L
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.8 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat. (dissolved fraction)
- Remarks:
- The WAF of different congeners C19Cl4 - C19Cl9 has been determined, the mean concentration was 0.8 mg/L. Specifically, the concentration in C19Cl4 was 0.9, in C19Cl5 0.07, in C19Cl6 0.03, in Cl19Cl7 0.02, in C19Cl8 0.02, in C19Cl9 0.03.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Validity criteria fulfilled:
- yes
- Conclusions:
- After 96h of exposure at the limit concentration of 1000 mg/L, the sample does not induce the mortality of any of the seven Zebrafish under test (0/7), that is an acceptable value for the limit test. Overall, LC50 @ 96h > 1000 mg/L.
- Executive summary:
This technical report summarizes all the data collected during the study: the exposure conditions of the fish at the tested sample limit concentration and the calculation of results. When using vertebrates for scientific purposes, as forced by the EU regulations, the 3 Rs rule must be obeyed. It includes three principles: Replacement, Reduction, and Refinement. Reduction refers to the use of methods that enable researchers to obtain comparable levels of information from fewer animals, or to obtain more information from the same number of animals. Whenever possible, for eco-tests on fishes, the limit test (one single concentration) is to be preferred to the serial concentrations test (at least five concentrations). Seven Zebrafishes, with a recommended total length of 1-2 cm, are exposed to the sample at the chosen limit concentration in breeding water, for a period of 96 hours. Mortality is recorded at 24, 48, 72 and 96 hours and compared with the negative control.
Reference
Description of key information
The substance has a nominal LC50 > 1000 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- > 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)