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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 3 h
- Dose descriptor:
- IC10
- Effect conc.:
- 6 000.2 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 5573.6 - 6426.8 ppm
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No data available.
- Effect concentrations exceeding solubility of substance in test medium: no effects observed for all tested concentrations. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes.
- Relevant effect levels: 3-Hour EC50 value was determined to be 15.5 mg/L. - Reported statistics and error estimates:
- Analysis of Consumption Data:
Only the linear section on the chart paper representing the oxygen consumption was used to evaluate the results. The chart readings were transformed into mg of O2 per liter consumed per hour (mg O2/L*hr). To calculate the two control respiration rates for each test material concentrations:
1 ─ 2 *100* RS/(Rc1+Rc2),
Where:
RS = oxygen consumption rate of the inoculum exposed to the test material;
Rc1 = oxygen consumption rate, control 1;
Rc2 = oxygen consumption rate, control 2.
In addition to the assessment of the test material, the reference toxicant results were similarly evaluated. The reference toxicant data were subject to probit analysis to calculate the EC50.
To calculate the NOAEC, a t test was performed.
Consumption data were evaluated following the guidelines of USEPA(1989) in order to estimate the concentration which would produce a10%inhibition of oxygen consumption. The estimate is produced using a linear interpolation and requires that the inhibition increases monotonically with increasing exposure concentration. If this condition is not met, the data are smoothed to satisfy the condition.
Because the test concentrations were not replicated, the Jackknife statistical method was used to calculate a variance and the 95% confidence interval for the IC10. This method consists of calculating an IC10 using the first control value, then the second control value and finally the mean of the two control values. Additionally, each test concentration is dropped from the calculation in turn. This results in a number of IC10 estimates. The variance in these resultant IC10 estimates is used to produce a confidence interval with the degree of freedom based on the number of estimates. - Validity criteria fulfilled:
- yes
- Conclusions:
- The calculated no-observed-adverse-effect concentration (NOAEC) based on inhibition compared to the control was calculated to equal 1000 ppm. The EC50 could not be calculated. The IC10 was calculated to equal 6000.2 ppm with a 95 % confidence interval of 5573.6 to 6426.8 ppm.
- Executive summary:
The potential impact of test material on microbial metabolism, as represented by the consumption of oxygen, was investigated using the "Activated Sludge, Respiration Inhibition Test" as prescribed by OECD (1984) and detailed in WCC Protocol OECD209 (Expanded Range Procedures). The test duration was a three-hour exposure period to the test material followed by up to ten minutes for the measurement of oxygen consumption. The study design was comprised of five nominal exposure concentrations: 1, 10, 100, 1000 and 10,000 ppm; a duplicate control group; and an assessment of the sensitivity of the inoculum used in the test to a reference toxicant (3,5-dichlorophenol).
The activated sludge respiration test with test material passed the quality control criteria for an acceptable test. The EC50calculated for the reference toxicant was 15.5 rng/L, within the acceptable range of 5 to 30 mg/L. The two control replicates produced oxygen consumption rates within the required 15 % of each other, 54.7 and 55.4 mg O2/L*hr.
The respiration rates of the sludge-associated microbes exposed to the five nominal concentrations of test material were 59.0, 50.7, 61.5, 66.4 and 47.2 mg O2/L*hr respectively. The calculated NOAEC based on inhibition compared to the control calculated to equal 1000 ppm. The EC50 for this material regarding the inhibition of metabolism, as represented by respiration, was greater than 10,000 ppm, the IC10was calculated to equal 6000.2 ppm with a 95 % confidence interval of 5573.6 to 6426.8 ppm.
Reference
Table 2: Oxygen consumption and Chemical-Induced Inhibition of Bacterial Respiration
Chemical Conc. (replicate #) |
O2Consumption (mg/L*hr) |
Percent Inhibition |
Negative controls |
|
|
Sample 1 |
54.7 |
─ |
Sample 2 |
55.4 |
─ |
Test material |
|
|
1 ppm (1) |
59.0 |
-7.2 |
10 ppm (1) |
50.7 |
7.9 |
100 ppm (1) |
61.5 |
-11.7 |
1000 ppm (1) |
66.4 |
-20.6 |
10,000 ppm (1) |
47.2 |
14.3 |
Positive control |
|
|
10 ppm |
41.1 |
24.8 |
20 ppm |
16.4 |
70.2 |
40 ppm |
6.2 |
88.7 |
Statistical analysis of the results
The resultant respiration rates were respectively: 59.0, 50.7, 61.5, 66.4, and 47.2 mg O2/L*hr after treated with 1, 10, 100, 1000 and 10000 ppm test material. These data translate into percent inhibitions of -7.2, 7.9, -11.7, -20.6, and 14.3 %. Because none of the test concentrations produced an inhibition greater than 50%, an EC50could not be calculated. Each respiration rate was statistically compared as a single value versus the mean of the controls following the of Sokal & Rohlf (1981) using a single-sidedttest. The results of thettest revealed that the 10,000 ppm test concentration expressed a statistically significant inhibition of oxygen consumption as compared to the controls. The discriminatory power of the test is low, however, due to having only one degree of freedom.
An IC10was calculated using a point estimate technique and Jackknifed to produce a variance from which a confidence could be derived. The result estimate for the IC10was 6000.2 ppm of the test material. The 95% confidence interval for this estimate is from 5573.6 to 6426.8 ppm.
Description of key information
The calculated no-observed-adverse-effect concentration (NOAEC) based on inhibition compared to the control was calculated to equal 1000 ppm. The EC50 could not be calculated. The IC10 was calculated to equal 6000.2 ppm with a 95 % confidence interval of 5573.6 to 6426.8 ppm.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
The potential impact of test material on microbial metabolism, as represented by the consumption of oxygen, was investigated using the "Activated Sludge, Respiration Inhibition Test" as prescribed by OECD (1984) and detailed in WCC Protocol OECD209 (Expanded Range Procedures). The test duration was a three-hour exposure period to the test material followed by up to ten minutes for the measurement of oxygen consumption. The study design was comprised of five nominal exposure concentrations: 1, 10, 100, 1000 and 10,000 ppm; a duplicate control group; and an assessment of the sensitivity of the inoculum used in the test to a reference toxicant (3,5-dichlorophenol).
The activated sludge respiration test with test material passed the quality control criteria for an acceptable test. The EC50calculated for the reference toxicant was 15.5 rng/L, within the acceptable range of 5 to 30 mg/L. The two control replicates produced oxygen consumption rates within the required 15 % of each other, 54.7 and 55.4 mg O2/L*hr.
The respiration rates of the sludge-associated microbes exposed to the five nominal concentrations of test material were 59.0, 50.7, 61.5, 66.4 and 47.2 mg O2/L*hr respectively. The calculated NOAEC based on inhibition compared to the control calculated to equal 1000 ppm. The EC50for this material regarding the inhibition of metabolism, as represented by respiration, was greater than 10,000 ppm, the IC10was calculated to equal 6000.2 ppm with a 95 % confidence interval of 5573.6 to 6426.8 ppm.
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