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Diss Factsheets
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EC number: 465-100-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes
Test material
- Test material form:
- solid: particulate/powder
- Details on test material:
- Batch number: 022401
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- Actual test substance concentration at the start and after approximately 4, 24, 48, 72 and 96 hours.
- Details on sampling:
- For determination of the actual test substance concentration samples were taken
immediately before the introduction of the fish and after approximately 4, 24, 48, 72 and 96
hours after begin of exposure.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Identity and concentration of auxiliary solvent for dispersal: No auxiliary solvent was used. The test substance was
dissolved in dilution water.
Test organisms
- Test organisms (species):
- other: Zebra-fish (Brachydanio rerio)
- Details on test organisms:
- Species: Zebrafish (Brachydanio rerio).
Supplier: Fa. Amazonas Aquarium, A-2840 Grimmenstein.
Receipt of animals: 22 June 2005.
Length (cm): 2.543 ± 0.053 (mean of control group at the end of study).
Weight (g): 0.436 ± 0.066 (mean of control group at the end of study).
As the allocation of the fish to their groups was performed using a randomisation procedure the size and the weight of the control fish is assumed to be representative for the entity of all fish used.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Remarks:
- Local tap water was mixed 1:1 with deionised water for achieving the appropriate water hardness (total hardness: 250 mg CaCO3 /L, determined in the control vessel).
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 250 mg CaCO3/L
- Test temperature:
- Temperature was measured with a general laboratory thermometer.
Temperature: 20 - 21 °C throughout the test period.
Determination of temperature daily. - pH:
- pH was determined with a pH-meter (WTW Hand Held Meter, pH 340, WTW G.m.b.H. D-82362 Weilheim i. OB).
pH: between 7.3 and 8.0 in the control medium and between 7.5 and 7.8 in the test substance medium. - Dissolved oxygen:
- Dissolved oxygen concentration in % of the air saturation value at the appropriate
temperature was determined with an oxygen meter (Oximeter 537, WTW G.m.b.H. D-82362
Weilheim i. OB).
Dissolved oxygen concentration: At least 90 % of the air saturation value at the appropriate temperature throughout the test period.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Details on results:
- In a preliminary test with 3 fish no fish died when exposed to a test substance concentration
of 100 mg/L. According to the test guideline a limit test may be performed at 100 mg/L in
order to demonstrate that the LC50 value is higher than this concentration.
The quality criteria were fulfilled:
• The mean concentration of the test substance has been satisfactorily maintained over a
test period of 96 hours. After 96 hours of exposure the geometric mean concentration
between the start and the end of the test was 102 % of the starting concentration.
• Constant conditions were maintained (temperature, pH).
• No mortality occurred in the negative control group.
• The dissolved oxygen concentration was higher than 60% of the air saturation value
throughout the test.
Actual test substance concentration
After 48 hours the actual test substance concentration was 103.6 mg/L (103.6 % of the
actual starting concentration) and it was 101.04 mg/L after 72 hours (101.0 % of the actual
starting concentration). At 96 hours the actual test substance concentration was 101.9 mg/L
(101.9 % of the actual starting concentration). The actual test substance concentrations
show that the test substance was stable during the whole experiment. For the 96-hours
exposure period the geometric mean concentration between start and end of the exposure
was 102.3 mg/L. The effective concentrations have been determined and expressed as the
geometric mean of the measured concentrations. This is in accordance with the criteria of the
respective guidelines.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Acute aquatic toxicity: Cat.3
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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