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EC number: 201-126-0 | CAS number: 78-59-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Restriction: only 4 Salmonella strains tested
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Salmonella mutagenicity tests: II. results from the testing of 270 chemicals.
- Author:
- Mortelmans K, Haworth S, Lawlor T, Speck W, Tainer B, Zeiger E
- Year:
- 1 986
- Bibliographic source:
- Environ Mutagen 8 Suppl. 7: 1-119
- Reference Type:
- review article or handbook
- Title:
- Toxicology and Carcinogenesis Studies of Isophorone in F344/N Rats and B6C3F1 Mice.
- Author:
- NTP (U.S. National Toxicology Program)
- Year:
- 1 986
- Bibliographic source:
- Techn Rep Ser No. 291, U.S. Department of Health and Human Services
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- 4 strains tested instead of 5
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3,5,5-trimethylcyclohex-2-enone
- EC Number:
- 201-126-0
- EC Name:
- 3,5,5-trimethylcyclohex-2-enone
- Cas Number:
- 78-59-1
- Molecular formula:
- C9H14O
- IUPAC Name:
- 3,5,5-trimethylcyclohex-2-enone
- Details on test material:
- Purity ≥ 97%
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat or hamster liver S9
- Test concentrations with justification for top dose:
- TA 100 and TA 1535 : 0 - 10000 μg/plate
TA 98 and TA 1537: 0 - 3333 μg/plate - Vehicle / solvent:
- water
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Remarks:
- potassium chloride
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- Strains TA 1535 and TA 100, without metabolic activation
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Remarks:
- potassium chloride
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-o-phenylenediamine
- Remarks:
- Strain TA 98, without metabolic activation
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Remarks:
- potassium chloride
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Strain TA 1537, without metabolic activation
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Remarks:
- potassium chloride
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- All strains, with metabolic activation
- Details on test system and experimental conditions:
- - Number of replicates: 3 per dose level, repeated
- Solvent: H2O
- 3 investigations were performed:
1. without metabolic activation
2. with metabolic activation (Arochlor-1254 liver rats)
3. with metabolic activation (Arochlor-1254 liver hamster)
- Cells and test compound or solvent (water) were incubated for 20 minutes at 37 ºC in the presence of either S9 or buffer. After the addition of soft agar, the contents of each tube were poured onto minimal medium, and the plates were incubated at 37 °C for 48 hours.
- The analysis was performed twice, each in triplicates.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: -S9: TA100, 1535, 98 at concentrations >=1000 µg/plate; +S9 (rat and hamster): only TA1535 at 10000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- True negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- The test substance was not mutagenic in strains TA100, TA1535, TA1537, or TA98 of Salmonella Typhimurium in the presence or absence of Aroclor 1254-induced rat (r.) or hamster (h.) liver S9.
Differences were not significant.
Maximum number of revertants in the standard plate test:
TA100:
Vehicle Test substance (µg/plate)
-S9 +S9(r.) +S9(h.) -S9 +S9(r.) +S9(h.)
-----------------------------------------------------------
82±4.7 92±7.5 79±2.2 88±7.4(333) 90±2.2(100) 117±2.9(333)
TA1535:
Vehicle Test substance (µg/plate)
-S9 +S9(r.) +S9(h.) -S9 +S9(r.) +S9(h.)
---------------------------------------------------------
6±2.5 7±0.9 7±1.2 4±0.9(100) 9±2.8(333) 6±1.0(100)
TA1537:
Vehicle Test substance (µg/plate)
-S9 +S9(r.) +S9(h.) -S9 +S9(r.) +S9(h.)
----------------------------------------------------------
2±0.3 3±1.5 4±0.7 4±0.7(1000) 5±1.0(100) 6±2.0(1000)
TA98:
Vehicle Test substance (µg/plate)
-S9 +S9(r.) +S9(h.) -S9 +S9(r.) +S9(h.)
----------------------------------------------------------
10±2.0 11±2.9 17±1.0 12±2.3(100) 17±1.3(100) 15±0.9(333)
The analysis was performed twice, each in triplicate; because the results were similar, data from only one experiment are shown.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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