Reaction mass of 1,3-diisopropylbenzene and 1,4-diisopropylbenzene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

1976

Materials and methods

Test guideline

Qualifier:

no guideline followed

Guideline:

other: Study from 1976 (no guidelines available at the time the study was performed).

Principles of method if other than guideline:

6 male SD rats were exposed (whole body) to 2100 mg TS/m3

GLP compliance:

no

Remarks:

Study from 1976 (GLP was not compulsory at the time the study was performed).

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of 1,3-diisopropylbenzene and 1,4-diisopropylbenzene

EC Number:

905-459-9

Molecular formula:

C12H18

IUPAC Name:

Reaction mass of 1,3-diisopropylbenzene and 1,4-diisopropylbenzene

Details on test material:

Lot/batch No.: XF 958

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Details on test animals or test system and environmental conditions:

No further data

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

not specified

Vehicle:

air

Details on inhalation exposure:

Initial sample: 107.2 g
Recovered sample: 104.1 g
Condensed sample: 0.0 g
Vaporised sample: 3.1 g
Chamber temperature: 25 degrees C
Chamber humidity: 80 %
Chamber volume: 35 L
Air flow rate: 4.0 L/min

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

6 h

Concentrations:

2100 mg/m3

No. of animals per sex per dose:

6

Control animals:

not specified

Statistics:

No data

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LC0

Effect level:

2 100 mg/m³ air

Based on:

test mat.

Exp. duration:

6 h

Mortality:

0/6

Clinical signs:

other: none

Body weight:

No data

Gross pathology:

14 days: viscera appeared normal

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The LC0 value for male rats was > 2100 mg/m3 for 6h exposure, which corresponds to CL50 / 4h > 3,15 mg/kg. As for cumene (isopropylbenzene) the LC0 is > 5.5 mg/L mist it is anticipated that DIPB LC50 is >5 mg/kg mist.

Executive summary:

In this older study 6 male Sprague-Dawley strain albino rats were exposed to 2100 mg TS/m3 (whole body) over 6 hrs. None of the animals died and the viscera appeared normal 14 days after dosing. This correspond to CL0 / 4h > 3,15 mg/kg and certainly to a CL50 > 5 mg/L mist with dermal exposure.

There are 2 studies with DIPB and Cumene, indicating LC0 respectively at 2100 mg/m3 (6h) and 17600 mg/m3 (1h), while they have vapour pressure of 0.34 and 4.9 hPa. We do not know really if they are based on saturating Vps, anyway needing to heat the substance which will condensate to aerosol when reaching the rat whole exposure cages. We can estimate the 4 hours value with the Haber law, either with C.t=K or C3.T=K In the first case CL0 would be 3.15 and 5.18 mg/L respectively. In the second case the CL0 will be for DIPB and Cumene of 2.41 and 11.1 mg/L. From these data it is assumed that the CL50 of DIPB is > 5 mg/L aerosol, leading to no CLP classification.