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EC number: 948-085-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07. Aug. 2008 - 04. Jun. 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- OECD Guideline for Testing of Chemicals, No. 202, Daphnia sp., Acute Immobilisation Test, 2004.
- Qualifier:
- according to guideline
- Guideline:
- other: Directive 92/69/EEC
- Version / remarks:
- EU Commission Directive 92/69/EEC, C.2, Acute Toxicity for Daphnia, 1992.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Copper di-D-gluconate
- EC Number:
- 208-408-2
- EC Name:
- Copper di-D-gluconate
- Cas Number:
- 527-09-3
- Molecular formula:
- C12H22CuO14
- IUPAC Name:
- copper(2+) bis(2,3,4,5,6-pentahydroxyhexanoate)
- Reference substance name:
- Sodium sulphate
- EC Number:
- 231-820-9
- EC Name:
- Sodium sulphate
- Cas Number:
- 7757-82-6
- Molecular formula:
- H2O4S.2Na
- IUPAC Name:
- disodium sulfate
- Reference substance name:
- Copper sulphate
- EC Number:
- 231-847-6
- EC Name:
- Copper sulphate
- Cas Number:
- 7758-98-7
- Molecular formula:
- CuSO4
- IUPAC Name:
- copper(2+) ion sulfate
- Test material form:
- liquid
Constituent 1
Constituent 2
Constituent 3
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- Dosage
A stock solution with a nominal concentration of 44 mg/L was prepared by completely dissolving 44.3 mg of test item in 1000 mL of test water using ultrasonic treatment for 10 minutes and intense stirring for 10 minutes at room temperature. This intensively mixed stock solution was used in a series of dilutions to prepare the test media of all test item concentrations. The test media were prepared just before introduction of the daphnids (i.e., start of the test).
See more information in the study report attach
Test solutions
- Vehicle:
- not specified
- Details on test solutions:
- Study Design
A static test without test medium renewal was performed. The following nominal concentrations of test item were tested: 0.10, 0.22, 0.46, 1.0 and 2.2 mg/L. Additionally, a control (test water without test item) was tested in parallel. The selection of the test concentrations was based on the results of range-finding tests (non-GLP).
For each treatment, 20 daphnids were used divided into four replicates of five daphnids each.
The volume of test solution provided for each daphnia was 10 mL. Thus, the requirement of the test guidelines for the minimum volume of 2 mL test medium per daphnia was fulfilled. The daphnids were randomly distributed to the test vessels at initiation of the test.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna Straus
- Strain/clone: clone 5 (young daphnids of the species Daphnia magna Straus)
Test system
The study was performed with young daphnids of the species Daphnia magna Straus. A clone of this species (defined by the supplier as clone 5) was originally supplied by the University of Sheffield / UK in 1992. Since that time, the clone has been bred at Harlan Laboratories in reconstituted water of the quality identical to the water quality used in the tests (in respect to pH, main ions, and total hardness) and under temperature and light conditions identical to those of
the tests (see below).
At the start of the test, the organisms used in the test were 6-24 hours old and were not first brood progeny.
For evaluation of the quality of the daphnia clone and the experimental conditions, potassium dichromate is tested as a positive control twice a year. The result of the latest positive control test in October 2008 (48-hour EC50: 0.70 mg/L, Harlan Laboratories Study C25711) indicated that the sensitivity of the test organisms was within the historical range of the Harlan Laboratories (48-hour EC50 from 1996 to 2008: 0.53-1.1 mg/L).
The test method and the test species are recommended by the international test guidelines.
Study design
- Test type:
- not specified
- Water media type:
- not specified
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Not specified
- Test temperature:
- The water temperature was maintained at 20 °C. Specified in table 3 ( page 18 of the study report).
- pH:
- Specified in table 3 (page 18 of the study report).
- Dissolved oxygen:
- Specified in table 3 (page 18 of the study report).
- Salinity:
- Not specified
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Specified in table 3 (page 18 of the study report).
- Details on test conditions:
- Experimental Conditions
The test was performed in a temperature-controlled room with continuous monitoring of the room temperature. The water temperature was maintained at 20 °C.
A 16-hour light to 8-hour dark cycle with a 30-minute transition period was used. Light intensity during the light period was approximately between 520 and 680 Lux.
The daphnids were not fed during the test. - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.59 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.22 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.22 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- not specified
- Details on results:
- RESULTS AND DISCUSSION
No analytical work was performed in agreement with the Sponsor.
The biological results (based on nominal test concentrations) are listed in Table 1.
During the first 24 hours of the test, no immobilized test organisms were determined in the
control and up to and including the test item concentration of 0.22 mg/L. At the next higher
concentrations of 0.46, 1.0 and 2.2 mg/L, the immobilization was 15, 40 and 90%, respectively.
The 24-hour EC50 of the test item was calculated to be 1.0 mg/L with 95% confidence limits of
0.36 and 3.1 mg/L. The 24-hour EC0 was 0.22 mg/L. The 24-hour EC100 was >2.2 mg/L.
After 48 hours of exposure, no immobilized test organisms were determined in the control and up to and including the test item concentration of 0.22 mg/L. At the next higher concentrations of 0.46 and 1.0 mg/L, the immobilization increased to 35 and 85%, respectively. At the highest test concentration of 2.2 mg/L, all test organisms were found to be immobile.
The 48-hour EC50 was calculated to be 0.59 mg/L with 95% confidence limits of 0.25 and
1.4 mg/L. The 48-hour EC0 and the 48-hour NOEC (highest concentration tested without toxic effects after 48 hours) of test item were both 0.22 mg/L, since no immobilization was observed up to and including this test concentration. The 48-hour EC100 was 2.2 mg/L.
No remarkable observations were made concerning the appearance of the test media. All test media were clear solutions throughout the entire test duration (Table 2).
At the beginning and end of the test period, the dissolved oxygen concentrations in the test media and control were at least 8.2 mg/L, the pH value of the test media was in the range of 7.8 to 8.0, and the water temperature during the test was 20 °C (Table 3). - Results with reference substance (positive control):
- The biological test results (based on nominal test concentrations) were as follows:
- 24-hour EC50: 1.0 mg/L
(95% confidence limits: 0.36 and 3.1 mg/L)
- 24-hour EC0: 0.22 mg/L
- 24-hour EC100: >2.2 mg/L
- 48-hour EC50: 0.59 mg/L
(95% confidence limits: 0.25 and 1.4 mg/L)
- 48-hour EC0 and 48-hour NOEC: 0.22 mg/L
- 48-hour EC100: 2.2 mg/L
Any other information on results incl. tables
See the tables in the study report from page 16 to page 18
Table 1 Effect of Sergomil L60 on the Mobility of Daphnia magna
Table 2 Appearance of the Test Media during the Test Period
Table 3 Dissolved Oxygen Concentrations, pH Values, and Temperature in the Treatments
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (48h) =0.59 mg/L (<1mg/L). Substance classifies as Aq. Acute tox 1.
- Executive summary:
The acute toxicity of the test item to Daphnia magna was determined in a 48-hour static test according to the EU Commission Directive 92/69/EEC, Part C.2 and the OECD Guideline for Testing of Chemicals, No. 202 (2004).
The nominal test item concentrations tested were 0.10, 0.22, 0.46, 1.0 and 2.2 mg/L. Additionally, a control group was tested in parallel.
No analytical work was performed in agreement with the Sponsor.
The biological test results (based on nominal test concentrations) were as follows:
- 24-hour EC50: 1.0 mg/L
(95% confidence limits: 0.36 and 3.1 mg/L)
- 24-hour EC0: 0.22 mg/L
- 24-hour EC100: >2.2 mg/L
- 48-hour EC50: 0.59 mg/L
(95% confidence limits: 0.25 and 1.4 mg/L)
- 48-hour EC0 and 48-hour NOEC: 0.22 mg/L
- 48-hour EC100: 2.2 mg/L
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