Phosphonium, triphenyl(3,7,11-trimethyl-2,4,6,10-dodecatetraenyl)-

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

triphenyl[(2E,4E,6E)-3,7,11-trimethyldodeca-2,4,6,10-tetraen-1-yl]-λ⁵-phosphane

EC Number:

603-493-6

Cas Number:

131560-11-7

Molecular formula:

C33 H38 P

IUPAC Name:

triphenyl[(2E,4E,6E)-3,7,11-trimethyldodeca-2,4,6,10-tetraen-1-yl]-λ⁵-phosphane

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the test substance was added to the test medium
- Eluate: no
- Differential loading: no
- Controls: 3 blanc controls
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): not specified

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Activated sludge from a laboratory STP fed with municipal waste water.
The inoculum was washed, brought to a concentration of 5 g/1 dry substance and aerated for 24 hours.
50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/1 dry substance in the test.inoculu

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test conditions

Test temperature:

20 +/- 2 °C

pH:

6.4 - 6.6 before correction; 7.1 - 7.3 after correction;7.5 at test end

Dissolved oxygen:

7.5 - 8.2 mg/L

Nominal and measured concentrations:

nominal: 0, 1, 10, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: erlenmeyer vessel
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass,, nominal 250 ml; 250 ml fill volume
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates):
- Sludge concentration (weight of dry solids per volume): 1g dry weight/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 209


OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Photoperiod: not applicable
- Light intensity:not applicable

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen consumption after 30 min
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10

Results and discussion

Effect concentrations

Key result

Duration:

30 min

Dose descriptor:

EC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Reported statistics and error estimates:

not applicable