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EC number: 446-810-1 | CAS number: 136239-66-2 1-BUTOXY-2,3-DIFLUORBENZOL; BUPHOL
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 FEB 2003 - 12 MAY 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- OECD Guideline for Testing of Chemicals No. 301 F: "Ready Biodegradability: Manometric Respirometry Test", adopted July 17, 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- Commission Directive 92/69/EEC, Method C.4-D of July 31, 1992: Manometric Respirometry Test (EEC Publication No. L 383 A, December 1992)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hess. Ministerium für Umwelt, Landwirtschaft und Forsten, Mainzer Strafle 80, 65189 Wiesbaden, Germany, 10. Juni 2002
Test material
- Reference substance name:
- 1-butoxy-2,3-difluorobenzene
- EC Number:
- 446-810-1
- EC Name:
- 1-butoxy-2,3-difluorobenzene
- Cas Number:
- 136239-66-2
- Molecular formula:
- Hill formula: C10H12F2O CAS formula: C10H12F2O
- IUPAC Name:
- 1-butoxy-2,3-difluorobenzene
- Test material form:
- liquid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge, micro organisms from a domestic waste water treatment plant, supplied by the sewage plant Groß-Zimmern, Germany.
- Preparation of inoculum for exposure: The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and again centrifuged. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 1.5 g dry material per litre were mixed with test water and then aerated until use.
- Concentration of sludge: 1.5 g dry material per litre
- Water filtered: no - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- OECD TG 301 F
TEST CONDITIONS
- Test temperature: 21 °C
- pH: 7.7 - 7.9
- pH adjusted: no
- Aeration of dilution water: continous stirring
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Manometric Test System with test flasks containing a volume of 500mL
- Number of culture flasks/concentration: 2 (Test item), 2 (Inoculum control), 1 (Procedure control, Abiotic control, Toxicity control)
- Method used to create aerobic conditions: continous stirring
- Measuring equipment: The change of pressure in the test flasks was measured by means of a manometric method (BSB/BOD-Sensor-System, Aqualytic, 63231 Neu Isenburg, Germany) each day.
- CO2 trap: Evolved carbon dioxide was absorbed in an aqueous solution (45 %) of potassium hydroxide
- Test performed in open system: no
CONTROL AND BLANK SYSTEM
- Inoculum blank: Activated sludge at a concentration of 30 mg suspended solids per litre was filled up with test water to a volume of 244 mL.The oxygen demand of the inoculum control (medium and inoculum) was 30 mg O2/L and thus not greater than 60 mg O2/L within 28 days.
- Abiotic sterile control: 25.4 mg of test item filled up with test water (sterile filtered, 0.2 - 0.45 µm filter) to a volume of 244 mL.
- Toxicity control: 25.7 mg of the test item), 25.4 mg Aniline and activated sludge at a concentration of 30 mg suspended solids per litre were filled up with test water to a volume of 244 mL.
- Other: Procedure Control: 25.7 mg Aniline and activated sludge at a concentration of 30 mg suspended solids per litre were filled up with test water to a volume of 244 mL. The procedure control was also used for other projects which ran in parallel.
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 7
- Sampling time:
- 28 d
- Details on results:
- Points of degradation plot (test substance):
7 % degradation after 28 d
BOD5 / COD results
- Results with reference substance:
- Points of degradation plot (reference substance):
91 % degradation after 14 d
101 % degradation after 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.
- Executive summary:
Purpose
The test item was investigated for its ready biodegradability in a Manometric Respirometry Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the micro organisms during exposure. As a reference item Aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.The GLP study was perfomred according to OECD TG 301F.
Result
Biodegradation of test item
After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28 -day exposure period the degradation of 7 % was found.
Biodegradation of Aniline
The reference item Aniline was sufficiently degraded to 91 % after 14 days, and to 101 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.
Biodegradation in the Toxicity Control
In the toxicity control containing both, the test item and the reference item aniline, 44 % biodegradation was noted within 14 days and 52 % biodegradation was determined after 28 days of incubation. Thus, the test item can be assumed to be not inhibitory on the activated sludge micro organisms.
Conclusion
The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.
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