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EC number: 425-560-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 5 MAY 2000 - 28 AUG 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The study was performed in compliance with the Good Laboratory Practice (GLP) regulations (revised in 1997, ENV/MC/CHEM(98)17). The method followed that described in the OECD Guidelines for Testing of Chemicals (Adopted: 4 April 1984) No 202 "Daphnia sp., Acute Immobilisation Test and Reproduction Test".
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium (reconstituted water and test material) was freshly prepared. Therefore, the calibrated flask with test material and vehicle, reconstituted water, was treated in an ultrasonic device for 1 hour. Subsequently the preparation was aerated, and stirred with a magnetic stirrer for further 23 hours. After 24 hours the formulation was given through a nutsch filter (pore size >10 - <16 µm). The filtrate was used for the study. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea, Daphnia magna (Straus)
- Age at study initiation (mean and range, SD): not older than 24 h
- Stage and instar at study initiation: Newborn animals were separated and allocated to the different dose groups.
- Source: in house breeding
- Age of parental stock (mean and range, SD): The strain with the parent generation was bred and maintained in vessels containing a batch of Daphnia magna of different ages.
- Feeding during test : no
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 14 ° dH (about 250 mg/L CaCO3)
- Test temperature:
- 22-23 °C
- pH:
- 7.9 +/- 0.3
- Dissolved oxygen:
- at least 90 %
- Nominal and measured concentrations:
- limit test at 0.1 g/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass vessel
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, 25 mL
- Volume of solution: 10 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
Reconstituted water according to ELENDT (1990):
Macro nutrients (mg/L):
CaCI2 x 7 H2O 293.80
MgSO4 x 7 H2O 123.30
NaHCO3 64.80
KCI 5.80
Na2SiO3 x 9H2O 10.00
NaNO3 0.27
KH2PO4 0.14
K2HPO4 0.18
Trace elements (mg/L):
B 0.5000
Fe 0.2000
Mn 0.1000
Li, Rb and Sr 0.0500
Mo 0.0250
Br 0.0125
Cu and Zn 0.0063
Co and I 0.0025
Se 0.0010
V 0.0003
Macro nutrients (mg/L):
Na2EDTA x 2H2O 2.50
Vitamins (µg/L):
Thiamine 75.00
B12 1.00
Biotin 0.75
After preparation, the reconstituded water was aerated for 24 hours.
- Hardness: about 14° dH (about 250 mg/L CaCO3)
- pH: 7.9 ± 0.3, after an aeration for 24 hours.
References:
ELENDT, B.-P. Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus.
Protoplasma 154, 25-33, 1990
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light - 8 hours
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : EC50 (24, 48 h)
VEHICLE CONTROL PERFORMED: yes
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.1 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.1 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control:
none
- Immobilisation of control: none
In the control and in an aqueous solution of the test material concentration of nominal 0.1 g/L (limit test), no immobilized test animals were observed during the test period of 48 hours.
No remarkable observations were made concerning the appearance of the solution of the test material preparation. All test media were clear preparations and stayed unchanged throughout the study. - Results with reference substance (positive control):
- The sensitivity of the test system has to be demonstrated periodically. The positive control was carried out with potassium dichromate.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48 hour EC50 for the test material to Daphnia magna could not be quantified due to the absence of toxicity at the highest test concentration achievable in water. An aqueous solution of 0.1 g/L revealed no aquatic toxicity in the test system.
- Executive summary:
The registered substance was tested for toxicity to invertebrates in an acute immobilisation test according to OECD Guideline 202 following GLP.
Purpose
The purpose of this assay was to identify the aquatic toxicity potential of the test material in Daphnia magna to provide a rational basis for hazard estimation for the test item in aquatic environments.
Study Design
For this purpose, juvenile daphnids were exposed to an aqueous test material solution over 48 hours, under defined conditions. The study comprised of four test vessels per concentration and control group containing five daphnids each, i.e. 20 daphnids per concentration (test medium group) and control group.
Daphnids were exposed to a nominal test material concentration of 100 mg/L (limit test) in an open static system.
Results
The analysis of a saturated aqueous solution revealed that the water solubility was < 0.00004 g/L.
The limit of quantification of the analytical method was described to be about 0.000133 g/L. Therefore the test material concentrations in the aqueous medium at the start and the end of the study could not be quantified.
After an exposure to a saturated aqueous solution of a nominal concentration of 0.1 g/L for 48 hours to Daphnia magna the following results were obtained:
Nominal concentration Immobilized/exposed daphnids [mg/L] 24 hours 48 hours 0 0/20 0/20 100 0/20 0/20
Daphnia magna exposed to an aqueous solution of the nominal test item concentration of 0.1 g/L were not affected.
For the test material, the following nominal EC50 values for daphnids were determined:
24 h EC50 > 100 mg/L
48 h EC50 > 100 mg/L
Conclusion
The 48 hour EC50 for the test material to Daphnia magna could not be quantified due to the absence of toxicity at the highest test concentration achievable in water. An aqueous solution of 0.1 g/L revealed no aquatic toxicity in the test system.
Reference
Table 1: Immobilisation of daphnids at a test item concentration of 0 and 100 mg/L (nominal) after 24 and 48 h
Nominal concentration | Immobilized/exposed daphnids | |
[mg/L] | 24 hours | 48 hours |
0 | 0/20 | 0/20 |
100 | 0/20 | 0/20 |
Description of key information
Key, acute immobilisation, D. magna, limit test, OECD 202, GLP: EC50 > 100 mg/L (nominal), NOEC > 100 mg/L (nominal) after 48 h.
Key value for chemical safety assessment
Additional information
Acute immobilisation, OECD 202
The registered substance was tested for toxicity to invertebrates in an acute immobilisation test according to OECD Guideline 202 following GLP.
Purpose
The purpose of this assay
was to identify the aquatic toxicity potential of the test material in Daphnia
magna to provide a rational basis for hazard estimation for the test
item in aquatic environments.
Study Design
For this purpose, juvenile daphnids were exposed to an
aqueous test material solution over 48 hours, under defined conditions.
The study comprised of four test vessels per concentration and control
group containing five daphnids each, i.e. 20 daphnids per concentration
(test medium group) and control group.
Daphnids were exposed to a nominal test material concentration of 100
mg/L (limit test) in an open static system.
Results
The analysis of a saturated aqueous solution revealed that
the water solubility was < 0.00004 g/L.
The limit of quantification of the analytical method was described to be
about 0.000133 g/L. Therefore the test material concentrations in the
aqueous medium at the start and the end of the study could not be
quantified.
After an exposure to a saturated aqueous solution of a nominal
concentration of 0.1 g/L for 48 hours to Daphnia magna the
following results were obtained:
Nominal concentration | Immobilized/exposed daphnids | |
[mg/L] | 24 hours | 48 hours |
0 | 0/20 | 0/20 |
100 | 0/20 | 0/20 |
Daphnia magna exposed to an
aqueous solution of the nominal test item concentration of 0.1 g/L were
not affected.
For the test material, the following nominal EC50 values for daphnids
were determined:
24 h EC50 > 100 mg/L
48 h EC50 > 100 mg/L
Conclusion
The 48 hour EC50 for the test material to Daphnia
magna could not be quantified due to the absence of toxicity at the
highest test concentration achievable in water. An aqueous solution of
0.1 g/L revealed no aquatic toxicity in the test system.
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