Acetic acid, C11-14-branched alkyl esters, C13-rich

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18 November 1985 to 20 November 1985 and 02 December 1985 to 04 December 1985

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Acetic acid, C11-14-isoalkyl esters, C13-rich

EC Number:

283-740-9

EC Name:

Acetic acid, C11-14-isoalkyl esters, C13-rich

Cas Number:

84712-50-5

Molecular formula:

C15H30O2

IUPAC Name:

Acetic acid, C11-14-isoalkyl esters, C13-rich

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: A colorless liquid, Batch I


STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sampling method: Samples were taken from all test concentrations (pooled replicates) on Day 0, before adding test organisms and at termination. BW2 for test material preparation and
stock solutions were also sampled during the study. All samples were collected in duplicate.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The water soluble fraction of MRD-84-521 was prepared by combining approximately 16.75 ml of the test material with 2.5 liters of BW2 in a carboy. The mixture was then stirred for approximately 72 hours on a stirring platform. After the mixing period, the 100% WSF was drawn off through a sampling tube for use as the stock solution.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Cultured in environmental laboratory
- Age of parental stock (mean and range, SD): Trial 2 from 15 day old parents
Trial 3 from 13 day old parents
- Feeding during test: Not fed

ACCLIMATION
- Acclimation period: Acclimation by rearing
- Type and amount of food: Cultures of Daphnia were fed Selenastrum capricornutum and a trout chow mixture. Algae were cultured in the Aquatic Laboratory using modified ASM-1 media.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

156 to 166 mg/L

Test temperature:

Trial 2- 20.73 +/- 0.54°C
Trial 3 20.92 +/- 0.41°C

pH:

8.1 to 8.5

Dissolved oxygen:

8.3 to 9.5 mg/L

Nominal and measured concentrations:

Nominal: 0, 6.25, 12.5, 25, 50, 100 % WSF

Details on test conditions:

TEST SYSTEM
- Test vessel: The test chambers were 400 ml glass chambers containing a volume of 250 ml of test solution.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- Biomass loading rate: Loading during the study did not exceed 40 daphnids per liter of test solution.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours dark
- Light intensity: Trial 2- Range 74 to 80 footcandles, Trial 3- Range 78-85 footcandles

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilization


RANGE-FINDING STUDY
Based on previous study 252142

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 other: % WAF

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Details on results:

There was insufficient mortality in Trial 2 to calculate an LC50 value. There was no mortality in Trial 3. No calculations were performed and no statistical analysis is presented.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

Based on the estimated 48-h LC50 of >100% (WAF), the test material is not classified as acutely toxic to freshwater algae according to Regulation (EC) No. 1272/2008 (CLP).

Executive summary:

There was insufficient mortality in Trial 2 to calculate an LC50 value. There was no mortality in Trial 3. No calculations were performed and no statistical analysis is presented. Therefore a 48 -h LC50 of >100% (WAF) is considered.