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EC number: 207-055-1 | CAS number: 429-06-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 8 October - 23 October 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- OECD Guideline 301F, Section 3. Degradation and Accumulation. "Ready Biodegradability: Manometric Respirometry Test" (adopted July 17, 1992).
- GLP compliance:
- yes
Test material
- Reference substance name:
- tetraethylammonium tetrafluoroborate
- Cas Number:
- 429-06-1
- Molecular formula:
- C8H20BF4N
- IUPAC Name:
- tetraethylammonium tetrafluoroborate
- Test material form:
- solid: crystalline
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: The source of test organisms was activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Storage conditions: The freshly obtained sludge was kept under continuous aeration until further treatment. Before use, the sludge was coarsely sieved (1 mm) and washed with mineral medium.
- Concentration: After treatment, the concentration of suspended solids (SS) was determined to be 3 g/L in the concentrated sludge as used for the test. The sludge was used as inoculum at a concentration of 2 mL per litre of mineral medium, leading to a final concentration SS of 6 mg/L.
- Preparation of inoculum for exposure: At the start of the test (day 0) mineral medium was inoculated with activated sludge.
At the start of the test (day 0), test and/or reference item were added to the respective bottles. The volumes of suspensions were made up to 360 mL or 244 mL using inoculated mineral medium. Rubber gaskets containing two pellets of sodium hydroxide were inserted into each bottle. Subsequently, each bottle was sealed by screwing an automated respirometer head on top. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 39 mg/L
- Based on:
- ThOD
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: see materials and methods section
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 22-23 °C
- pH: The pH measured in all flasks at the start of the test was 7.7. At the end of exposure (Day 28), pH values in the range of 7.7 to 8.5 were measured.
- Suspended solids concentration: the concentration of suspended solids was determined to be 3 g/L in the concentrated sludge as used for the test.
TEST SYSTEM
- Test vessels: 500 mL brown colored glass bottles. The end volume of medium was 360 mL or 244 mL, in order to obtain the required headspace-to-volume ratio.
- Milli-RO water: Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon.
- Stock solutions of mineral components:
A) 8.50 g KH2PO4
21.75 g K2HPO4
67.20 g Na2HPO4.12H2O
0.50 g NH4Cl
dissolved in Milli-RO water and made up to 1 litre, pH 7.4 ± 0.2
B) 22.50 g MgSO4.7H2O dissolved in Milli-RO water and made up to 1 litre.
C) 36.40 g CaCl2.2H2O dissolved in Milli-RO water and made up to 1 litre.
D) 0.25 g FeCl3.6H2O dissolved in Milli-RO water and made up to 1 litre.
- Mineral medium: 1 litre mineral medium contains: 10 mL of solution (A), 1 mL of solutions (B) to (D) and Milli-RO water.
- CO2 absorber: Sodium hydroxide pellets (Merck, Darmstadt, Germany).
- Illumination: The test media were excluded from light.
- Respirometer: Lovibond BD600-GLP manometric respirometry system, equipped with an inductive stirring system.
Reference substance
- Reference substance:
- other: Sodium acetate
Results and discussion
- Preliminary study:
- NDA
- Test performance:
- The ThOD of Tetraethylammonium tetrafluoroborate was calculated to be 2.10 mg O2/mg.
The ThOD of sodium acetate was calculated to be 0.78 mg O2/mg.
The relative biodegradation values calculated from the measurements performed during the test period based on ThOD, respectively 1% for bottle A and 2% for bottle B.
In the toxicity control, more than 25% biodegradation occurred within 14 days (54%, based on ThOD).
Functioning of the test system was checked by testing the reference item sodium acetate.
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1 - <= 2
- Sampling time:
- 28 d
- Details on results:
- The relative biodegradation values calculated from the measurements performed during the test period based on ThOD, respectively 1% for bottle A and 2% for bottle B.
BOD5 / COD results
BOD5 / COD
- Parameter:
- ThOD
- Value:
- 2.1 other: mg O2/mg
- Results with reference substance:
- The ThOD of sodium acetate was calculated to be 0.78 mg O2/mg.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Tetraethylammonium tetrafluoroborate was not readily biodegradable under the conditions of the Manometric Respirometry Test presently performed.
- Executive summary:
The objective of the study was to evaluate the test item Tetraethylammonium tetrafluoroborate for its ready biodegradability in an aerobic aqueous medium with microbial activity introduced by inoculation with activated sludge; Manometric Respirometry Test.
Test duration was 28 days.
The relative biodegradation values calculated from the measurements performed during the test period revealed no biologically relevant biodegradation of Tetraethylammonium tetrafluoroborate, based on ThOD (1% and 2% for bottle A and B, respectively).
In the toxicity control, Tetraethylammonium tetrafluoroborate was found not to inhibit microbial activity.
Since all criteria for acceptability of the test were met, this study was considered to be valid.
In conclusion, Tetraethylammonium tetrafluoroborate was designated as not readily biodegradable.
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