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EC number: 909-129-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: New Guidebook for Mutagenicity Tests using Microorganisms, compiled by Chemical Substance Research Division, Safety and Health Department, Ministry of Labor
- Version / remarks:
- 1990
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Reaction mass of m,alpha-dimethylstyrene and p,alpha-dimethylstyrene
- EC Number:
- 909-129-5
- Molecular formula:
- C10H12
- IUPAC Name:
- Reaction mass of m,alpha-dimethylstyrene and p,alpha-dimethylstyrene
- Test material form:
- liquid
- Details on test material:
- Indentification
Cas: 26444-18-8
Purity: 97.35%
batch: A94-015
Storage conditions: Not specified
Constituent 1
Method
- Target gene:
- - S. typhimurium: Histidine gene
- E. coli: Tryptophan gene
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat liver S9-mix induced by a combination of phenobarbital and benzoflavone
- Test concentrations with justification for top dose:
- Dose setting study (without and with S9) for all strains: 5, 10, 50, 100, 500, 1000, and 5000 µg/plate
Growth inhibition was observed for TA98, TA100, TA1535 and TA1537 at 50 µg/plate and for WP2uvrA at 100µg/plate.
Main study: (without and with S9) TA100, WP2uvrA TA1535, TA1537 and TA98: 0.781, 1.56, 3.13, 6.25, 12.5, 25, 50, 100 µg/plate. - Vehicle / solvent:
- - solvent used: DMF
- Justification for choice of solvent: DMF is an appropiate solvent for this assay.
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide
- Remarks:
- without S9, 0.01 µg/plate in DMSO for TA100
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- without S9, 0.5 µg/plate in DMSO for TA1535
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: ICR-191
- Remarks:
- without S9, 1 µg/plate in DMSO for TA1537
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide
- Remarks:
- without S9, 0.01 µg/plate in DMSO for WP2uvrA
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide
- Remarks:
- without S9, 0.1 µg/plate in saline for TA98
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- with S9. in DMSO for tester strains, TA98 0.5µg/plate, TA1535 2 µg/plate, TA1537 2 µg/plate, WP2uvrA 10 µg/plate
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- no
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- With S9, 5 µg/plate in DMSO for TA100
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar
DURATION
- Exposure duration: 48 hour
NUMBER OF REPLICATIONS:
- One dose setting and one main experiment, both in duplicate
NUMBER OF CELLS EVALUATED: 10E8 per plate
DETERMINATION OF CYTOTOXICITY
- Method: Observing if growth inhibition has occurred by counting a decrease in revertants
OTHER EXAMINATIONS:
- not performed - Evaluation criteria:
- A test substance is considered positive if:
The result of the mutagenicity test on the test substance was concluded to be positive if the number of the colonies of a revertant that had appeared was not less than two times as many as that of the colonies of a revertant appearing in the case of the solvent controls and there was a dose-response relationship observed.
Further, if it was confirmed that there had been no entry of any unwanted bacteria in the sterility test, the number of the colonies of a revertant of the solvent control substances and the positive control substances was within the range of the historical background data and there were no less than four concentration groups that show growth inhibition of bacteria, then the test was judged to be valid.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
Cytotoxicty was observed in the dose setting test.
TA1535,TA1537, TA98, TA100, without and with S9: 50 µg/plate and above.
WP2uvrA, without and with S9: 100 µg/plate and above.
Applicant's summary and conclusion
- Conclusions:
- In an AMES test, similar to OECD 471 Isopropenyl toluene (mixture of m- and p-isomer) was found not to be mutagenic with or without metabolic activation.
- Executive summary:
An AMES test was performed similair to OECD 471. Cytoxicity was observed for the strains TA1535,TA1537, TA98, TA100 at 50 µg/plate and above with and without S9 and for WP2uvrA at 100 µg/plate and above with and without S9. No significant dose-related increase in the number of revertants with or without metabolic activation was seen beneath the concentrations that induced growth inhibition. Based on the results of this study it is concluded that Isopropenyl toluene (mixture of m- and p-isomer) is not mutagenic in the Salmonella typhimurium reverse mutation assay and in the Escherichia coli reverse mutation assay with or without metabolic activation.
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