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EC number: 618-797-4 | CAS number: 91893-72-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-06-12 to 2018-10-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Preparation of inoculum for exposure: The activated sludge was washed twice with chlorine free tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration with CO2 free air untii test start (2 days). The amount of inoculum used to initiate inoculation was 4.0 mL/L (25.0 mg/L dw).
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 46 mg/L
- Based on:
- test mat.
- Remarks:
- corresponding to an oxygen demand of 51.1 mg O2/L
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral salts medium according to OECD 301 F
- Test temperature: Nominal: 20 - 24 ± 1 °C; Actual: 21.0 - 21.2 °C
- pH: 7.42 - 7.71
- pH adjusted: no
- Suspended solids concentration: 25.0 mg/L dw
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Brown glass bottles (volume 500 mL), continuous stirring
- Number of culture flasks/concentration:
- two for the inoculum control (C1, C2)
- one for the functional control (R1)
- two for the test item (P1, P2)
- one for the toxicity control (T1);
Separate replicates of test item and toxicity control were prepared for measurement of the pH-value at test start:
- one for the test item (PpH)
- one for the toxicity control (TpH)
- Measuring equipment:
OxiTop® OC1 10 controller with software Achat OC, WTW
OxiTop® measuring heads, WTW
Stirring platform, WTW
Climatic exposure test cabinet, RUMED RUBARTH APPARATE GMBH
pH-Meter, Multi 350i, WTW
Datalogger 174 T, TesTo
Analytical balance, SARTORlUS
Balance, KERN
Magnetic stirrer, M83000, lab 4 you
Spectralphotometer NANOCOLOR® UV/VlS, MACHEREY- NAGEL
Test Kits: Nitrat 50, Nitrit 2, all by MACHEREY-NAGEL
Multipette X-Stream, EPPENDORF
Various pipettes
- Details of trap for CO2 and volatile organics if used: A rubber sleeve with soda lime was hung into the opening of the bottles to absorb evolved CO2. The bottles were closed with OxiTop® measuring heads and the measuring system was activated.
CONTROL AND BLANK SYSTEM
- Inoculum blank: duplicate, The inoculated test medium, consisting of the required volumes of mineral medium stock solutions, ultrapure water and inoculum, was prepared in a measuring flask. 250 mL of this solution were filled in the brown glass bottles of the inoculum control, using a 250 mL measuring flask.
- Functional control: one test vessel, the reference item was weighed out and transferred into a measuring flask, the required volumes of mineral medium stock solutions, ultrapure water and inoculum, were added. 250 mL of this solution were filled in the brown glass bottle of the functional control, using a 250 mL measuring flask.
- Toxicity control: one test vessel, the test item was weighed out and directly transferred into the test vessels with 250 mL of the reference item medium, using a 250 mL measuring flask. - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 18
- Sampling time:
- 15 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 67
- Sampling time:
- 23 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 93
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- second test vessel
- Value:
- 13
- Sampling time:
- 17 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- second test vessel
- Value:
- 66
- Sampling time:
- 27 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- second test vessel
- Value:
- 79
- Sampling time:
- 28 d
- Results with reference substance:
- % degradation on day 1: 10
% degradation on day 10: 93
% degradation on day 28: 100
The pass level for ready biodegradation (≥ 60% degradation) was reached within 3 days. The validity criterion that the degradation should be ≥ 60% after 14 d was fulfilled. Stages of Biodegradation: The adaptation phase changed to the degradation phase within 1 day (degradation > 10%), with a maximum of 100% on day 15 - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- After a test period of 28 days, the test item is classified as readily biodegradable within the 10-day-window and within the 28 day period of the study.
Reference
Description of key information
The ready biodegradability of the test item 2H-lsoindole-2 -ethanesulfonic acid, 1,3 -dihydro-1,3 -dioxo-, potassium salt (1:1) (TA-1) was determined with non-adapted activated sludge in the Manometric Respirometry Test for a period of 28 days according to OECD guideline 301 F. The validity criteria of the guideline are fulfilled. The test item is classified as readily biodegradable within the 10 -day-window and within the 28 day period of the study.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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