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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1969
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: comparable to guideline study with acceptable restrictions; only 4 male animals per dose were used; Regarding the occlusive dressing the test protocol used was harsher; no necropsy performed

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Range finding toxicity data: List VI
Author:
Smyth H.F. et al.
Year:
1962
Bibliographic source:
J. Amer. Ind. Hyg. Assoc. J. 23: 95
Reference Type:
publication
Title:
Range finding toxicity data: List VII
Author:
Smyth H.F. et al.
Year:
1969
Bibliographic source:
J. Amer. Ind. Hyg. Assoc. J. 30: 470

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Deviations:
yes
Remarks:
only 4 male animals per dose were used; occlusive dressing; no necropsy performed
Principles of method if other than guideline:
Method is described in the publication of Smyth et al (cf. Reference)
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Cyclopentene
EC Number:
205-532-9
EC Name:
Cyclopentene
Cas Number:
142-29-0
Molecular formula:
C5H8
IUPAC Name:
cyclopentene
Details on test material:
no further data available
Specific details on test material used for the study:
Not specified

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
male
Details on test animals or test system and environmental conditions:
- Weight at study initiation: 2.5 - 3.5 kg

Administration / exposure

Type of coverage:
occlusive
Vehicle:
not specified
Details on dermal exposure:
Refer to the publication of Smyth et al (cf. Reference)
Duration of exposure:
24 hours
Doses:
As no information about the number of administered dose levels is given, the total amount of animals could not be determined.
No. of animals per sex per dose:
4
Control animals:
not specified
Details on study design:
The fur is removed from the entire trunk by clipping and the dose is retained beneath an impervious plastic film.
The animals were immobilized during the contact period; 14 days post-exposure observation period.
Statistics:
Not specified

Results and discussion

Preliminary study:
Not applicable.
Effect levels
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
1 231 mg/kg bw
Mortality:
No mortalities.
Clinical signs:
other: Irritation on uncovered rat belly = 5
Gross pathology:
Not specified
Other findings:
Not specified

Any other information on results incl. tables

Value of LD50 given in the literature was 1.59 ml/kg. With respect to the density of cyclopentene (0.774 g/ml), the value in mg/ml refers to 1231 mg/kg.

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Remarks:
according to EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulation (EC) No. 1272/2008
Conclusions:
Based on the results of the study, cyclopentene had a single skin penetration LD50 in rabbits of 1231 mg/kg bw.
Executive summary:

A range-finding toxicity study was conducted with New Zealand white rabbits using a variety of substances, including the test substance, cyclopentene. After a 24 -hour exposure to cyclopentene was shown to have a single skin penetration LD50 of 1231 mg/kg bw.