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Reaction mass of 5,5'-[vinylenebis[(3-sulpho-p-phenylene)azo]]bis[3-methylsalicylic] acid, potassium salt, compound with 2,2',2''-nitrilotriethanol and potassium 5-amino-3-{[4-(2-{4-[(7-amino-1-hydroxy-3-sulfo-2-naphthyl)diazenyl]-2-sulfophenyl}vinyl)-3-sulfophenyl]diazenyl}-4-hydroxy-7-sulfonaphthalene-2-sulfonate - 2,2',2''-nitrilotriethanol (1:1) and 3,3'-[vinylenebis[(3-sulpho-p-phenylene)azo]]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonic] acid, potassium salt, compound with 2,2',2''-nitrilotriethanol and 3,3'-[vinylenebis[(3-sulpho-p-phenylene)azo]]bis[6-amino-4-hydroxynaphthalene-2-sulphonic] acid, potassium salt, compound with 2,2',2''-nitrilotriethanol
EC number: 943-311-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-04-06 to 2017-05-31
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances):
The preliminary test (non-GLP)
The preliminary test was performed using the following test item concentrations: 120, 12, 1.2, 0.12 mg/L plus the control. The test item in the amount of 61.2 mg was weighed in a glass crystallizer and quantitatively transferred into flask by multiple washing with the Elendt M7 medium and filled up to 510 mL [SOP/W/7]. The test item concentration of 120 mg/L was visually homogeneous and colored black,
however, sufficiently transparent for an observation of the test organisms. Lower test item concentrations were prepared by sequential dilutions with the Elendt M7 medium in a ratio 1:9 (v/v)
(i.e. 50 mL of higher test item concentration was mixed with 450 mL of the Elendt M7 medium). The color intensity was correlated with the test item concentration.
The definitive test
The definitive test was performed with a single test item concentration of 120 mg/L as a limit test plus the control. The limit test concentration of 120 mg/L was selected to ensure that the mean measured
concentration is above 100 mg/L even in case of slight acceptable deviations from the nominal value. The test item in the amount of 60.1 mg was weighed in a glass crystallizer and quantitatively
transferred into flask by multiple washing with the Elendt M7 medium and filled up to 500 mL [SOP/W/7], (Table 3). The test item concentration of 120 mg/L was visually homogeneous and colored
black, however, sufficiently transparent for an observation of the test organisms. The control was Elendt M7 medium 500 mL). From the test item concentration and the control 50 mL were taken as a
sample for chemical determinations at exposure initiation [SOP/W/83]. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: Standard laboratory culture maintained in the Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology, Laboratory of Aquatic Toxicology [SOP/W/67]
- Age at study initiation (mean and range, SD): < 24 hours
- Method of breeding: Daphnia magna was cultured in glass beakers of 150 mL volume (one parent per vessel) at room temperature 18 – 22o C with the light : dark ratio of 16 h : 8 h (electronic time programmer, KANLUX, Poland). The culture was maintained in Elendt M7 medium [SOP/W/63, SOP/W/67].
- Feeding during test: none
ACCLIMATION
- Acclimation conditions (same as test or not): yes
- Type and amount of food: Pseudokirchneriella subcapitata algae suspension. Group B vitamins and micronutrients necessary for proper growth were supplied with lyophilized Spirulina sp. suspension. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 18.9-20.7
- pH:
- 7.64-7.75
- Dissolved oxygen:
- 8.9-9.0
- Conductivity:
- 521 µS/cm
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Type (delete if not applicable): covered with transparent lids in order to minimize evaporation and to prevent accidental contamination
- Material, size, headspace, fill volume: 150 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16:8 light:dark
TEST CONCENTRATIONS
- Spacing factor for test concentrations: limit test
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 120, 12, 1.2, 0.12 mg/L plus the control.
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: nominal concentrations verified analytically
- Validity criteria fulfilled:
- yes
Reference
Description of key information
Immobilization of Daphnia magna exposed to the test item, Direct Black 18L NA active dye, was investigated during a 48 -hour static test. The definitive test was performed with a single test item concentration of 120 mg/L as limit test. Four replicates were used for the test item concentration and the control, each with five Daphnia magna. Daphnia magna were observed for immobilization after 24 and 48 h of exposure. During exposure, no immobilization of Daphnia magna was observed in the control and in the test item concentration of 120 mg/L. The test item concentration was stable under test conditions. The mean measured concentration in the limit test concentration was 106.8 mg test item/L and 90.6 mg main ingredient/L. The endpoint values were determined based on the nominal test item concentrations.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 120 mg/L
Additional information
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