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EC number: 256-190-2 | CAS number: 45103-58-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13th-16th April 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Samples of the test solutions were collected to measure concentrations of the test substance at 0 (test initiation, Day 0) and 72 hours (exposure termination, Day 3). Duplicate samples were collected at approximately 0 and 72 hours with one set of samples processed immediately for analysis and the other set stored refrigerated for potential future analysis if deemed necessary by the Study Director. Samples collected at test initiation (0 hour) were collected from the individual batches of test solution prepared for each treatment and control group prior to distribution into the test chambers.
At exposure termination (72 hours), samples were collected from the pooled replicates from each treatment and control group. At each sampling interval, 8.0 mL of test solution was added to 20 mL glass scintillation vials containing 2.0 mL of 0.5% formic acid in methanol. - Vehicle:
- no
- Details on test solutions:
- A primary stock was prepared at a nominal concentration of 100 mg a.i./L by diluting 0.1002g of 2 (2-methoxyethoxy) ethyl methacrylate to a final volume of 1000 mL with freshwater AAP medium. The 100 mg a.i./L primary stock was stirred with a Teflon-coated stir bar and magnetic stir plate for twenty minutes and inverted at least twenty times to mix. The 100 mg a.i./L primary stock appeared clear and colorless with foam on the surface and continued stirring while all subsequent dilutions were made. Aliquots of the 100 mg a.i./L primary stock were diluted with freshwater AAP medium to prepare test solutions at nominal concentrations of 6.3, 13, 25, and 50 mg a.i./L. All test solutions appeared clear and colorless and otherwise unremarkable at the time of preparation.
- Test organisms (species):
- other: Raphidocelis subcapitata
- Details on test organisms:
- Algal cells for this study were taken from a culture that had been transferred to fresh medium four days prior to test initiation.The algal cells were cultured and tested in freshwater AAP medium (3). Stock nutrient solutions were prepared by adding reagent-grade chemicals to purified EAG Laboratories-Easton well water (NANOpure® water). The test medium then was prepared by adding appropriate volumes of the stock nutrient solutions to NANOpure® water. The pH of the medium was adjusted to 7.5 ± 0.1 with 10% hydrochloric acid and/or 0.1N sodium hydroxide at the time of preparation. The medium was sterilized by filtration (0.22 µm) and stored refrigerated prior to use.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Hardness:
- Not stated
- Test temperature:
- Temperatures remained within the 24 ± 2°C range established for the test.
- pH:
- The pH of all test solutions at test initiation was 7.3. At test termination, pH in the pooled biotic replicates of each respective treatment and control group ranged from 8.2 to 9.9. The observed increase in pH is typical for tests conducted with R. subcapitata and is attributed to the photosynthetic activity of the algae.
- Dissolved oxygen:
- Not stated.
- Salinity:
- Not stated.
- Conductivity:
- Not stated.
- Nominal and measured concentrations:
- Nominal test concentrations used in the definitive test were 6.3, 13, 25, 50, and 100 mg/L and were based upon the results of the range-finding test. Measured concentrations of 2-(2-methoxyethoxy) ethyl methacrylate were determined from samples of test medium collected from each treatment and control groups at 0 and 72 hours of the test.
- Details on test conditions:
- Test chambers were held in an environmental chamber at a temperature of 24 ± 2ºC. The temperature of a container of water adjacent to the test chambers in the environmental chamber was measured continuously using an Amega Scientific Corporation centralized monitoring system. The algae were held under continuous cool-white fluorescent lighting throughout the test. The target light intensity was 6,000 lux ± 10%. Light intensity was measured at test solution level at five locations surrounding the test flasks at test initiation using a SPER Scientific 840006C light meter. The pH of the medium in each treatment and control group was measured at test initiation and exposure termination using a Thermo Orion A214 pH meter. At test initiation, pH was measured in the individual batches of test solution prepared for each treatment and control group. At exposure termination, pH was measured in pooled samples of test solution collected from each of the replicates of each treatment and control group.
- Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 99 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 47 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 99 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 99 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Details on results:
- Conditions for the Validity of the Test
1) The mean cell density in the negative control replicates increased by a factor greater than 16 within three days. The factor was 343.
2) The coefficient of variation of the average specific growth rate in the negative control replicates during the whole test period did not exceed 7%. It was 1.55%.
3) The mean coefficient of variation for section by section specific growth rates (days 0 1, 1 2, and 2 3) in the negative control replicates did not exceed 35%. It was 13.3%.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The objective of this study was to determine the toxicity of 2-(2-methoxyethoxy) ethyl methacrylate, to the freshwater alga, Raphidocelis subcapitata, over a 72-hour exposure period using a study method based on OECD TG 201. The 72hour EC50, EyC50, and ErC50values were all estimated to be >99 mg a.i./L. The 72-hour NOEC was determined to 47 mg a.i./L.
- Executive summary:
In accordance with OECD TG 201 following GLP conditions, the freshwater alga, Raphidocelis subcapitata, was exposed to a geometric series of five mean, measured concentrations of2-(2-methoxyethoxy) ethyl methacrylateranging from 5.8 to 64 mg a.i./L in a study based on OECD TG 201. Effects were evaluated based on cell density, yield, and growth rate usingmean measuredtest concentrations. The 72‑hour EC50, EyC50, and ErC50values were all estimated to be >99 mg a.i./L. The 72-hour NOEC was determined to 47 mg a.i./L.
Reference
Description of key information
In accordance with OECD TG 201 following GLP conditions, the freshwater alga, Raphidocelis subcapitata, was exposed to a geometric series of five mean, measured concentrations of 2-(2-methoxyethoxy) ethyl methacrylateranging from 5.8 to 64 mg a.i./L in a study based on OECD TG 201. Effects were evaluated based on cell density, yield, and growth rate usingmean measuredtest concentrations. The 72‑hour EC50, EyC50, and ErC50values were all estimated to be >99 mg a.i./L. The 72-hour NOEC was determined to 47 mg a.i./L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 99 mg/L
- EC50 for marine water algae:
- 99 mg/L
- EC10 or NOEC for freshwater algae:
- 47 mg/L
- EC10 or NOEC for marine water algae:
- 47 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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