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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report date:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
GLP compliance:
yes

Test material

1
Chemical structure
Reference substance name:
Ginger, ext.
EC Number:
283-634-2
EC Name:
Ginger, ext.
Cas Number:
84696-15-1
Molecular formula:
N/A
IUPAC Name:
Essential oil extract of Zingiber officinalis (Zingiberaceae) root GINGER ESSENTIAL OIL Ginger Oil Ginger Oleoresin Ginger, ext Ginger, ext. Ginger, Extrakt HE Zingiber officinalis INCI name: Zingiber Officinale (Ginger) Root Extract OILS, GINGER Reaction mass of camphene and pin-2(3)-ene and 7-methyl-3-methyleneocta-1,6-diene and pin-2(10)-ene and cineole and [S-(R*,S*)]-5-(1,5-dimethylhexen-4-yl)-2-methyl-1,3-cyclohexa-1,3-diene and (R)-p-mentha-1,8-diene Zingiber officinalis, Zingiberaceae.

Test organisms

Test organisms (species):
Selenastrum sp.

Study design

Test type:
semi-static
Water media type:
saltwater
Limit test:
yes
Total exposure duration:
72 h
Remarks on exposure duration:
24, 48 and 72 hours

Test conditions

Test temperature:
21 °C ± 2 °C.
pH:
7.78

Results and discussion

Effect concentrations
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (total fraction)
Basis for effect:
growth rate

Any other information on results incl. tables

       Conc.                                      72%
       OD              Algal Biomass              Soecific Growth Rate 48 -72 hours
 1 2   3  1  2  3  mean  CV%  1  2  3  mean  CV%
 Neg control  0.782  0.779 0.816   916026.73 912390.92  957232.59  928550.08   2.68  1.25 1.2  1.23   1.23  2.05
 1000mg/l 0.146   0.15  0.155  145234.8  150082.55 156142.24   150486.53  3.63  -0.47  -0.46  -0.36  -0.43  14.15

    Conc.              Specific growth rate 0 -72 hours           Growth Inhibition%  
 1  2  3  mean  CV%  1  2  3  mean  CV%
 Neg. Control  1.51  1.5  1.52  1.51  0.66           /  
 1000 mg/l  0.89  0.9  0.92  0.9  1.70  41.06%  40.40%  39.07%  40.18%  2.52

    Conc.              Yield              Yield Inhibition (%)
 1  2  3  mean  CV%  1  2  3  mean  CV%
 Neg. Control  906026,73  902390,92  947232,59  918550,08  2,71              /
 1000 mg/l  135234,8  140082,55  146142,24 140486,53   3,89  85,28%  84,75%  84,09%  84,71%  0,70

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
the concentrations used in this test were: 1.8 mg/l – 1 mg/l – 0.56 mg/l – 0.32 mg/l – 0.18 mg/l. Results of the tests were reported in AR 2017/025/B.
Conclusions:
According to OECD 201:2006 corr. 2011, under the test conditions applied, the EC50 of the test substance resulted to be > 66.94 mg/ml (effective titer obtained from TOC analysis at concentration of saturation 1000 mg/ml).