Reaction mass of 3,7-dimethyl-1-octyl acetate and citronellyl acetate

Short-term toxicity to aquatic invertebrates

Description of key information

The short-term toxicity to aquatic invertebrates is assessed based on read-across from the close structural analogues Citronellyl Acetate (CAS# 150 -84 -5) to Citronellyl Acetate Multi.

Acute Daphnia test with Citronellyl Acetate mono

For Citronellyl Acetate mono, the acute toxicity to aquatic invertebrates was examined in a study according to OECD TG 202, and in compliance with GLP criteria. Groups of Daphnia magna (4 replicates of 5), were exposed to a range of test substance solutions for 48 hours under semi-static conditions. As the test substance is poorly soluble in water, a saturated solution was prepared at a loading rate of 100 mg/L, and this solution further diluted by a factor 2 to obtain nominal target concentrations of 0 (control), 6.25, 12.5, 25, 50, and 100 mg/L. Nominal concentrations were analytically verified at the start and at the end of the 24-h renewal periods. The recovery of test item in fresh solutions was in the range 4.7 to 6.1% of nominal. In the 24 hour old solutions the measured concentrations were between 36.4 to 54.2% of the initial test concentration. Time-weighted average (TWA) concentrations were calculated to be 0, 0.202, 0.519, 1.077, 1.651, and 3.755 mg/L, and effect values expressed as such. Daphnids were observed for immobilization after 24 and 48 hours of exposure. The study is valid.

After 48 hours exposure, no immobilization of daphnids was observed in the control and at nominal test concentrations of 6.25 and 25.0 mg/L. At a nominal test concentration of 12.5 mg/L, immobilization was 5%, and in the test concentrations of 50 and 100 mg/L, immobilization was 35% and 50%, respectively. Based on these findings, the 48-h EC50 value was determined to be 3.48 mg/L based on measured concentrations.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

3.48 mg/L