Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 277-633-6 | CAS number: 73912-21-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-11-17 - 1997-11-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Study conducted under GLP according to EU method C.11 on the registered substance itself with marginal deficiencies in documentation in the test report; the available information allows to draw the conclusion that the test was conducted properly without deviation from the guideline.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- as set out in 88/302/EEC
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- not applicable
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: direct weighing
- Controls: yes
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none noted - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Pretreatment: none
- Initial biomass concentration: 400 mg/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Test temperature:
- 20±2°C
- pH:
- 7.5
- Dissolved oxygen:
- initially 5.1 - 6.3 mg/L
- Nominal and measured concentrations:
- 0, 100, 1000, 10000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Material, size, headspace, fill volume: total 250 ml fill volume
- Aeration: permanent for 30 min (incubation time)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: 400 mg/L
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Oxygen consumption
TEST CONCENTRATIONS
- Test concentrations: 0, 100, 1000, 10000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none noted
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: 5; 10; 20 mg/L led to a respiratory rate of 24.0; 8.6; 4.3 mg/L * h and a respiratory inhibition of 11.1; 68.1; 84.1%.; EC50 = 9.2 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The study was conducted under GLP according to EU method C.11 on the registered substance itself with marginal deficiencies in documentation in the test report; the available information allows to draw the conclusion that the test was conducted properly without deviation from the guideline. The method is considered reasonable and the reference substance produced the appropriate response. Hence, the results can be considered as reliable to assess the effects of 12H-Dibenzo[d,g][1,3,2]dioxa-phosphocin, 4,8-dicyclohexyl-6-hydroxy-2,10-dimethyl- to aquatic microorganisms, here, activated sludge. The determined EC values for respiratory inhibition, i.e. EC50 and EC10, were > 10000 mg/L, clearly indicating that the test substance does not inhibit respiration and shows the relative non-toxicity of 12H-Dibenzo[d,g][1,3,2]dioxa-phosphocin, 4,8-dicyclohexyl-6-hydroxy-2,10-dimethyl- towards microorganisms.
- Executive summary:
In a study according to EU method C.11 under GLP, a defined quantity of activated sludge is mixed with synthetic medium and the respiratory rate is measured under static conditions. This rate is compared to those measured in test preparations with various concentrations of 12H-Dibenzo[d,g][1,3,2]dioxa-phosphocin, 4,8-dicyclohexyl-6-hydroxy-2,10-dimethyl-, i.e. 100, 1000, and 10000 mg/L (nominal). In order to check the sensitivity of the activated sludge used, 3,5-dichlorophenol is used as a reference substance. The reference substance produced the appropriate results, the EC50and EC10values of 12H-Dibenzo[d,g][1,3,2]dioxa-phosphocin, 4,8-dicyclohexyl-6-hydroxy-2,10-dimethyl- were determined to be > 10000 mg/L. This toxicity study is classified as acceptable and satisfies the guideline requirement for a toxicity study in microorganisms.
Reference
Description of key information
Toxicity to microorganisms: 0.4 g/L activated sludge of a predominantly domestic sewage, static, 30 min exposure, 0, 100, 1000, 10000 mg/L (nominal): EC10, EC50 > 10000 mg/l (EU method C.11, GLP)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 g/L
- EC10 or NOEC for microorganisms:
- 10 g/L
Additional information
Nominal concentration is above the water solubility of the test item: Its estimated water solubility is 1.171 E-5 mg/l and the test item needs to be considered as insoluble.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.