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EC number: 283-415-1 | CAS number: 84625-40-1 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Trigonella foenum-graecum, Leguminosae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2018-01-17 to 2018-04-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- This study is performed according to the OECD 301F guideline.
- Justification for type of information:
- Details on study plan is provided in this study record.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- None
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge, microorganisms from a domestic waste water treatment plant will be supplied by a municipal sewage treatment plant (e.g. Rossdorf, Germany).
- Conditioning:The aerobic activated sludge used for this study was deposited for 15 min, washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in test water and centrifuged again. This procedure was done three times. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 3.5 g dry material per litre were mixed with test water and aerated overnight. This suspension was used for the experiment.
The washed activated sludge will be pre-conditioned in test water for a maximum of 2 days. - Duration of test (contact time):
- >= 28 - <= 60 d
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Surrounding Type: Climatic chamber
- Temperature: 22 °C ± 1°C
- Light Conditions: Darkness
- pH-Value: 6.5 to 6.6 (measured at the start of the test and adjusted to 7.4 to 7.6 with Sodium Hydroxide) 7.4 to 7.8 (measured at day 63)
- Recording: Test conditions (temperature) will be recorded continuously with suitable instruments, documented in the raw data and reported in the final report. (Short-term deviations (< 2 hours) from the recommended temperature range will normally not result in major disturbances of the test performance and will not be recorded).
TEST WATER
- Reconstitued pure water: Analytical grade salts were added to pure water to prepare the following stock solutions:
a) 8.5 g KH2PO4, 21.75 g K2HPO4, 33.4 g Na2HPO4 x 2 H2O, 0.5 g NH4Cl filled up with pure water to 1000 mL volume; the pH should be 7.4 ± 0.2.
b) 22.5 g MgSO4 x 7 H2O filled up with pure water to 1000 mL volume
c) 36.4 g CaCl2 x 2 H2O filled up with pure water to 1000 mL volume
d) 0.25 g FeCl3 x 6 H2O filled up with pure water to 1000 mL volume
In order to avoid precipitation of iron hydroxide in the stock solution d), one drop of concentrated HCl per litre was added before storage.
50 mL of stock solution a) and 5 mL of the stock solutions b) to d) were combined and filled up to a final volume of 5000 mL with pure water.
COURSE OF THE TEST
- Preparation of Test Flasks: The amounts of test item and reference item were directly weighed into the test flasks.
For a better distribution of the test item, silicone oil AR 20 at a concentration of about 1% of the final flask volume was be added to all flasks in order to improve the bioavailability of the mixture constituents.
- The closed test flasks were incubated in a climatised chamber under continuous stirring. The consumption of oxygen was determined by measuring the change of pressure in the flasks. Evolved carbon dioxide was absorbed in an aqueous solution (45%) of potassium hydroxide.
- Test Duration: 60 days (pH was measured at day 63)
TEST PARAMETERS
- Measurements of Oxygen: The change of pressure in the test flasks was measured by means of a manometric method (BSB/BOD-Sensor-System, Aqualytic Dortmund, Germany) each day.
- Temperature: The temperature was recorded by means of the automated software AMR Wincontrol©.
- pH-Value: pH-values were measured in procedure control, a separately prepared test flask with test item (to prevent loss of test item in the test flasks) and a separately prepared test flask without test item (control) at test start and in all flasks at day 63, except in the abiotic and toxicity control, using a pH-electrode WTW pH 340i.
TEST SYSTEM
- The closed test flasks were incubated in a climatised chamber under continuous stirring. The consumption of oxygen was determined by measuring the change of pressure in the flasks. Evolved carbon dioxide was absorbed in an aqueous solution (45%) of potassium hydroxide.
- Number of culture flasks/concentration: 2
The test flasks were closed gas-tight by a measuring head. An appropriate CO2-absorber (KOH as an aqueous solution) was used for trapping the produced carbon dioxide. The amount of O2 consumed by the activated sludge was calculated from the decrease of pressure in the reaction vessel.
SAMPLING
O2 consumed daily by the activated sludge was calculated from the decrease of pressure in the reaction vessel.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 (activated sludge + silicone oil + test water)
- Abiotic sterile control: 1
- Toxicity control: 1
- Other: Procedure control : (Ref. Item + activated sludge + silicone oil + test water) - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- Sodium Benzoate
- Preliminary study:
- No data.
- Test performance:
- No data.
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- ThODNH4/ThODNO3
- Value:
- 10
- Sampling time:
- 1 d
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- ThODNO3
- Value:
- 60
- Sampling time:
- 11 d
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- ThODNH4
- Value:
- 61
- Sampling time:
- 11 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- ThODNO3
- Value:
- 70
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- ThODNH4
- Value:
- 72
- Sampling time:
- 28 d
- Details on results:
- The mean biodegradation of 10% of Fenugreek Absolute was reached at day 1 (ThODNH4, ThODNO3). At the end of the 10-day window at day 11, the mean degradation of Fenugreek Absolute was 61% (ThODNH4) and 60% (ThODNO3) and therefore the 10 day window criterion was passed. The mean biodegradation after 28 days was 72% (ThODNH4) and 70% (ThODNO3), and at test end after 60 days 82% (ThODNH4) and 80% (ThODNO3).
Therefore, Fenugreek Absolute is considered to be readily biodegradable based on ThODNH4 and ThODNO3. - Results with reference substance:
- The reference item sodium benzoate was sufficiently degraded to 85% after 14 days, to 89% after 28 days and to 103 % after 60 days of incubation.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The degradation rate of Fenugreek Absolute reached 60% within the 10-day window and after 28 and 60 days.
Therefore, Fenugreek Absolute is considered to be readily biodegradable based on ThODNH4 and ThODNO3. - Executive summary:
The test item Fenugreek Absolute was investigated for its ready biodegradability in a manometric respirometry test, according to the OECD 301F guideline and under GLP compliance. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
Nitrogen is part of the molecular structure of the test item; therefore nitrification (ThODNO3) was considered for the evaluation of the results. The criterion for ready biodegradability under the conditions of a manometric respirometry test is the degradation of the test item of at least 60%, reached within a 10-day window; the 10-day window starts when the degradation of the test item reaches at least 10% degradation.
The mean biodegradation of 10% of Fenugreek Absolute was reached at day 1 (ThODNH4, ThODNO3). At the end of the 10-day window at day 11, the mean degradation of Fenugreek Absolute was 61% (ThODNH4) and 60% (ThODNO3) and therefore the 10 day window criterion was passed. The mean biodegradation after 28 days was 72% (ThODNH4) and 70% (ThODNO3), and at test end after 60 days 82% (ThODNH4) and 80% (ThODNO3).
Therefore, Fenugreek Absolute is considered to be readily biodegradable based on ThODNH4and ThODNO3.
In the toxicity control containing both, the test item and the reference item sodium benzoate, 63% (ThODNH4) biodegradation was noted within 14 and 28 days (62% based on ThODNO3) and 62% after 60 days (ThODNH4) as well as 61% (ThODNO3).
According to the test guidelines, the test item can be assumed to be not inhibitory to the aerobic activated sludge microorganisms because degradation was >25% within 14 days.
Reference
pH-Values at the End of the Test
Flask No. |
Treatment |
pH-value |
1 |
Fenugreek Absolute |
7.4 |
2 |
Fenugreek Absolute |
7.6 |
3 |
Inoculum control |
7.5 |
4 |
Inoculum control |
7.5 |
5 |
Reference item (procedure control) |
7.8 |
Cumulative Biochemical Oxygen Demand (mg O2/L) in Test Flasks during the Test Period of 60 Days
Time |
Flask No. |
|||||||
(days) |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
|
1 |
30 |
30 |
0 |
0 |
60 |
0 |
55 |
|
2 |
55 |
55 |
5 |
0 |
85 |
0 |
95 |
|
3 |
80 |
75 |
5 |
5 |
105 |
0 |
125 |
|
4 |
95 |
100 |
5 |
5 |
120 |
10 |
155 |
|
5 |
110 |
115 |
5 |
5 |
130 |
20 |
200 |
|
6 |
120 |
130 |
10 |
5 |
135 |
25 |
210 |
|
7 |
130 |
140 |
10 |
10 |
140 |
30 |
225 |
|
8 |
135 |
150 |
10 |
10 |
145 |
30 |
230 |
|
9 |
145 |
160 |
10 |
10 |
150 |
35 |
240 |
|
10 |
150 |
170 |
15 |
10 |
150 |
35 |
250 |
|
11 |
155 |
175 |
15 |
10 |
150 |
35 |
265 |
|
12 |
160 |
175 |
15 |
10 |
155 |
35 |
275 |
|
13 |
165 |
175 |
15 |
10 |
155 |
40 |
280 |
|
14 |
170 |
175 |
20 |
10 |
160 |
45 |
280 |
|
15 |
175 |
175 |
20 |
10 |
160 |
45 |
280 |
|
16 |
175 |
180 |
20 |
15 |
160 |
45 |
280 |
|
17 |
180 |
180 |
25 |
15 |
165 |
45 |
280 |
|
18 |
180 |
180 |
25 |
15 |
165 |
45 |
280 |
|
19 |
185 |
180 |
25 |
20 |
165 |
45 |
280 |
|
20 |
185 |
185 |
25 |
20 |
170 |
50 |
285 |
|
21 |
190 |
185 |
25 |
20 |
170 |
50 |
285 |
|
22 |
190 |
190 |
25 |
20 |
170 |
50 |
285 |
|
23 |
195 |
190 |
25 |
20 |
170 |
50 |
285 |
|
24 |
195 |
195 |
25 |
20 |
170 |
50 |
285 |
|
25 |
195 |
195 |
25 |
20 |
170 |
50 |
285 |
|
26 |
195 |
195 |
25 |
20 |
175 |
55 |
285 |
|
27 |
200 |
200 |
25 |
20 |
175 |
55 |
285 |
|
28 |
205 |
200 |
25 |
20 |
175 |
55 |
285 |
|
29 |
210 |
205 |
25 |
20 |
180 |
55 |
285 |
|
30 |
210 |
205 |
25 |
20 |
180 |
55 |
285 |
|
31 |
210 |
205 |
25 |
20 |
180 |
55 |
285 |
|
32 |
210 |
205 |
25 |
20 |
180 |
55 |
285 |
|
33 |
210 |
205 |
25 |
20 |
180 |
55 |
285 |
|
34 |
215 |
205 |
25 |
20 |
180 |
55 |
285 |
|
35 |
215 |
210 |
25 |
20 |
185 |
55 |
285 |
36 |
215 |
210 |
25 |
20 |
185 |
55 |
285 |
37 |
220 |
210 |
25 |
20 |
190 |
60 |
285 |
38 |
220 |
215 |
25 |
20 |
190 |
60 |
285 |
39 |
220 |
215 |
25 |
20 |
190 |
60 |
285 |
40 |
220 |
215 |
25 |
20 |
190 |
60 |
285 |
41 |
220 |
215 |
25 |
20 |
190 |
60 |
285 |
42 |
220 |
215 |
25 |
20 |
190 |
60 |
285 |
43 |
220 |
215 |
25 |
20 |
190 |
60 |
285 |
44 |
220 |
215 |
25 |
20 |
195 |
65 |
285 |
45 |
220 |
220 |
30 |
20 |
195 |
65 |
285 |
46 |
220 |
220 |
30 |
20 |
195 |
65 |
285 |
47 |
220 |
220 |
30 |
20 |
195 |
65 |
285 |
48 |
225 |
225 |
30 |
20 |
195 |
65 |
285 |
49 |
230 |
230 |
30 |
20 |
195 |
65 |
285 |
50 |
230 |
230 |
30 |
20 |
195 |
65 |
285 |
51 |
230 |
230 |
30 |
20 |
195 |
65 |
285 |
52 |
230 |
230 |
30 |
20 |
195 |
65 |
285 |
53 |
230 |
230 |
30 |
20 |
195 |
65 |
285 |
54 |
230 |
230 |
30 |
20 |
195 |
65 |
285 |
55 |
230 |
230 |
30 |
20 |
195 |
65 |
285 |
56 |
230 |
230 |
30 |
20 |
195 |
65 |
285 |
57 |
230 |
230 |
30 |
20 |
195 |
65 |
285 |
58 |
230 |
230 |
30 |
20 |
195 |
65 |
285 |
59 |
230 |
230 |
30 |
20 |
200 |
65 |
285 |
60 |
230 |
230 |
30 |
20 |
200 |
65 |
285 |
Flasks 1 and 2: Fenugreek Absolute
Flasks 3 and 4: inoculum control
Flask5: reference(procedure control)
Flask 6: abiotic control
Flask 7: toxicity control
Percentage Biodegradation of Test Item, of Sodium Benzoate and of the Toxicity Control based on ThODNH4:
Time |
Percentage Biodegradation1 |
|||
(Days) |
Fenugreek Absolute1 |
Sodium Benzoate2 |
Toxicity |
|
|
Flask 1 [%] |
Flask 2 [%] |
Flask 5 [%] |
Flask 7 [%] |
1 |
12 |
12 |
35 |
13 |
2 |
21 |
21 |
48 |
22 |
3 |
30 |
28 |
59 |
29 |
4 |
36 |
39 |
67 |
36 |
5 |
41 |
45 |
73 |
47 |
6 |
44 |
50 |
75 |
48 |
7 |
47 |
53 |
76 |
51 |
8 |
49 |
57 |
79 |
52 |
9 |
53 |
61 |
82 |
55 |
10 |
54 |
64 |
81 |
57 |
11 |
56 |
66 |
81 |
60 |
12 |
58 |
66 |
83 |
63 |
13 |
60 |
66 |
83 |
64 |
14 |
61 |
65 |
85 |
63 |
15 |
63 |
65 |
85 |
63 |
16 |
62 |
66 |
83 |
63 |
17 |
63 |
65 |
85 |
62 |
18 |
63 |
65 |
85 |
62 |
19 |
64 |
64 |
83 |
61 |
20 |
64 |
66 |
86 |
63 |
21 |
66 |
66 |
86 |
63 |
22 |
66 |
68 |
86 |
63 |
23 |
68 |
68 |
86 |
63 |
24 |
68 |
70 |
86 |
63 |
25 |
68 |
70 |
86 |
63 |
26 |
68 |
70 |
89 |
63 |
27 |
70 |
72 |
89 |
63 |
28 |
72 |
72 |
89 |
63 |
29 |
74 |
74 |
92 |
63 |
30 |
74 |
74 |
92 |
63 |
31 |
74 |
74 |
92 |
63 |
32 |
74 |
74 |
92 |
63 |
33 |
74 |
74 |
92 |
63 |
34 |
76 |
74 |
92 |
63 |
35 |
76 |
76 |
95 |
63 |
36 |
76 |
76 |
95 |
63 |
37 |
78 |
76 |
98 |
63 |
38 |
78 |
78 |
98 |
63 |
39 |
78 |
78 |
98 |
63 |
40 |
78 |
78 |
98 |
63 |
41 |
78 |
78 |
98 |
63 |
42 |
78 |
78 |
98 |
63 |
43 |
78 |
78 |
98 |
63 |
44 |
78 |
78 |
101 |
63 |
45 |
77 |
79 |
100 |
62 |
46 |
77 |
79 |
100 |
62 |
47 |
77 |
79 |
100 |
62 |
48 |
79 |
81 |
100 |
62 |
49 |
81 |
83 |
100 |
62 |
50 |
81 |
83 |
100 |
62 |
51 |
81 |
83 |
100 |
62 |
52 |
81 |
83 |
100 |
62 |
53 |
81 |
83 |
100 |
62 |
54 |
81 |
83 |
100 |
62 |
55 |
81 |
83 |
100 |
62 |
56 |
81 |
83 |
100 |
62 |
57 |
81 |
83 |
100 |
62 |
58 |
81 |
83 |
100 |
62 |
59 |
81 |
83 |
103 |
62 |
60 |
81 |
83 |
103 |
62 |
1ThODNH4of
Fenugreek Absolute: 2.444 mg O2/mg test item
2ThODNH4of
sodium benzoate: 1.666 mg O2/mg reference item
Percentage Biodegradation of Test Item, of Sodium Benzoate and of the Toxicity Control based on ThODNO3:
Time |
Percentage Biodegradation1 |
|||
(Days) |
Fenugreek Absolute1 |
Sodium Benzoate2 |
Toxicity |
|
|
Flask 1 [%] |
Flask 2 [%] |
Flask 5 [%] |
Flask 7 [%] |
1 |
12 |
12 |
35 |
13 |
2 |
20 |
21 |
48 |
22 |
3 |
29 |
28 |
59 |
28 |
4 |
35 |
38 |
67 |
35 |
5 |
40 |
44 |
73 |
46 |
6 |
43 |
49 |
75 |
48 |
7 |
46 |
52 |
76 |
51 |
8 |
48 |
56 |
79 |
52 |
9 |
52 |
59 |
82 |
54 |
10 |
53 |
62 |
81 |
56 |
11 |
55 |
64 |
81 |
59 |
12 |
57 |
64 |
83 |
62 |
13 |
59 |
64 |
83 |
63 |
14 |
60 |
63 |
85 |
62 |
15 |
62 |
63 |
85 |
62 |
16 |
61 |
64 |
83 |
62 |
17 |
62 |
63 |
85 |
61 |
18 |
62 |
63 |
85 |
61 |
19 |
63 |
62 |
83 |
61 |
20 |
63 |
64 |
86 |
62 |
21 |
65 |
64 |
86 |
62 |
22 |
65 |
66 |
86 |
62 |
23 |
66 |
66 |
86 |
62 |
24 |
66 |
68 |
86 |
62 |
25 |
66 |
68 |
86 |
62 |
26 |
66 |
68 |
89 |
62 |
27 |
68 |
70 |
89 |
62 |
28 |
70 |
70 |
89 |
62 |
29 |
72 |
72 |
92 |
62 |
30 |
72 |
72 |
92 |
62 |
31 |
72 |
72 |
92 |
62 |
32 |
72 |
72 |
92 |
62 |
33 |
72 |
72 |
92 |
62 |
34 |
74 |
72 |
92 |
62 |
35 |
74 |
74 |
95 |
62 |
36 |
74 |
74 |
95 |
62 |
37 |
76 |
74 |
98 |
62 |
38 |
76 |
76 |
98 |
62 |
39 |
76 |
76 |
98 |
62 |
40 |
76 |
76 |
98 |
62 |
41 |
76 |
76 |
98 |
62 |
42 |
76 |
76 |
98 |
62 |
43 |
76 |
76 |
98 |
62 |
44 |
76 |
76 |
101 |
62 |
45 |
75 |
77 |
100 |
61 |
46 |
75 |
77 |
100 |
61 |
47 |
75 |
77 |
100 |
61 |
48 |
77 |
79 |
100 |
61 |
49 |
79 |
81 |
100 |
61 |
50 |
79 |
81 |
100 |
61 |
51 |
79 |
81 |
100 |
61 |
52 |
79 |
81 |
100 |
61 |
53 |
79 |
81 |
100 |
61 |
54 |
79 |
81 |
100 |
61 |
55 |
79 |
81 |
100 |
61 |
56 |
79 |
81 |
100 |
61 |
57 |
79 |
81 |
100 |
61 |
58 |
79 |
81 |
100 |
61 |
59 |
79 |
81 |
103 |
61 |
60 |
79 |
81 |
103 |
61 |
1ThODNO3of
Fenugreek Absolute: 2.502 mg O2/mg test item
2ThODNH4of
sodium benzoate: 1.666 mg O2/mg reference item
Biodegradation in the Toxicity Control
In the toxicity control containing both, the test item and the reference item sodium benzoate, 63% (ThODNH4) biodegradation was noted within 14 and 28 days (62% based on ThODNO3) and 62% after 60 days (ThODNH4) as well as 61% (ThODNO3).
Abiotic Control
The oxygen demand in the abiotic control started at day 44 and reached a maximum of 65 mg/L during the test duration of 60 days.
Validity criteria
Inoculum Control: |
The oxygen demand of the inoculum control (medium and inoculum) was 22.5 mg O2/L and thus not greater than 60 mg O2/L within 28 days as required by the test guideline. |
pH-Value: |
The pH-value of the test item flasks at day 63 was 7.4 to 7.6 and therefore within the range of pH 6.0 to 8.5 as required by the test guideline. |
Reference Item: |
The percentage degradation of the reference item should reach the level for ready biodegradability (>60%) within 14 days as required by the test guideline. The reference item sodium benzoate was degraded to more than 60% after 4 days of incubation. |
Test Item: |
The difference of duplicate values for the degradation of the test item at the end of the test, at day 28 and at the end of the 10-day window was less than 20%. The difference of duplicate values at day 11 was 10%, at day 28 0% and at day 60 2%. The validity criterion was fulfilled. |
Toxicity Control: |
If in a toxicity test, containing both the test item and a reference item less than 25% biodegradation (based on total ThOD) occurred within 14 days, the test item can be assumed to be inhibitory. The biodegradation was 63% (ThODNH4) and 62% (ThODNO3) at day 14; the test item was not inhibitory. |
Description of key information
The registered substance is regarded as readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
The test item Fenugreek Absolute was investigated for its ready biodegradability in a manometric respirometry test, according to the OECD 301F guideline and under GLP compliance. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
Nitrogen is part of the molecular structure of the test item; therefore nitrification (ThODNO3) was considered for the evaluation of the results. The criterion for ready biodegradability under the conditions of a manometric respirometry test is the degradation of the test item of at least 60%, reached within a 10-day window; the 10-day window starts when the degradation of the test item reaches at least 10% degradation.
The mean biodegradation of 10% of Fenugreek Absolute was reached at day 1 (ThODNH4, ThODNO3). At the end of the 10-day window at day 11, the mean degradation of Fenugreek Absolute was 61% (ThODNH4) and 60% (ThODNO3) and therefore the 10 day window criterion was passed. The mean biodegradation after 28 days was 72% (ThODNH4) and 70% (ThODNO3), and at test end after 60 days 82% (ThODNH4) and 80% (ThODNO3).
Therefore, Fenugreek Absolute is considered to be readily biodegradable based on ThODNH4and ThODNO3.
In the toxicity control containing both, the test item and the reference item sodium benzoate, 63% (ThODNH4) biodegradation was noted within 14 and 28 days (62% based on ThODNO3) and 62% after 60 days (ThODNH4) as well as 61% (ThODNO3).
According to the test guidelines, the test item can be assumed to be not inhibitory to the aerobic activated sludge microorganisms because degradation was >25% within 14 days.
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