3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooct-1-ene

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20 - 24 August 2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

Qualifier:

according to guideline

Guideline:

other: Fish, acute toxicity test stipulated in the "Testing methods for new chemical substances" (No. 1121002, Pharmaceutical and food safety bureau, Environmental policy bureau, Ministry of the environment, Japan)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: control, 100 mg/L

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was added into the dilution water in an Erlenmeyer flask to produce the 100 mg/L test solution. The flask was immediately sealed with a plug not to produce headspace. The solution was stirred by magnetic stirrer for approx. 48 h. After settling for approx. 1 h at 24 ± 1 °C the middle layer of the settled solution was used for testing. The test vessels were covered immediately with a glass lid not to produce headspace.
- Eluate: no
- Differential loading: yes
- Controls: yes, test medium control
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No, test solutions were clear and colorless at test start and before renewal.

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Medaka
- Source: Kurume Laboratory, Chemicals Evaluation and Research Institute, Fukuoka, Japan
- Length at study initiation (length definition, mean, range and SD): 2.3 ± 1.2 cm

ACCLIMATION
- Acclimation period: 27 d
- Acclimation conditions (same as test or not): same as test
- Type and amount of food during acclimation: feed mixture for carp (2C)
- Feeding frequency during acclimation: fed regularly but not fed 24 h before start of exposure
- Health during acclimation (any mortality observed): 0% mortality at 7 d before start of exposure

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

24.0 - 24.7 °C

pH:

7.5 - 7.8

Dissolved oxygen:

7.4 - 8.2 mg/L

Nominal and measured concentrations:

nominal: control, 100 mg/L
measured: < LOD, 0.117 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 3 L glass tanks (diameter: 16 cm, depth: 17 cm)
- Type (delete if not applicable): closed with glass lid to avoid headspace
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): every 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water according to guideline
- Culture medium different from test medium: no
- Intervals of water quality measurement: dissolved oxygen, pH and temperature were measured at test start, before and after renewal and at the end of exposure

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark
- Light intensity: artificieal light of white fluorescent lamps

EFFECT PARAMETERS MEASURED
- Mortality and visible abnormalities were recorded after 3, 24, 48, 72 and 96 h

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: control, 100 mg/L
- Results used to determine the conditions for the definitive study: No mortality was recorded.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.117 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: No abnormal responses were observed during exposure.
- Observations on body length and weight: length: 2.4 ± 0.11 cm; body length: 0.11 ± 0.013 g
- Mortality of control: 0%

Sublethal observations / clinical signs:

Table 1: Measured concentrations of test item in test solutions

Nominal concentration [mg/L]	Measured concentration [mg/L]
	At test start	24 h		48 h		72 h		At test end	Geometric mean
		Old	New	Old	New	Old	New
Control	< LOD	< LOD	< LOD	< LOD	< LOD	< LOD	< LOD	< LOD	< LOD
100	0.329	0.168	0.213	0.0880	0.0831	0.0666	0.0805	0.0768	0.117

Validity criteria fulfilled:

yes

Remarks:

0% mortality was recorded in the control.

Conclusions:

LC50 (96 h) > 0.117 mg/L (meas. geom. mean)

Description of key information

LC50 (96 h) > 100 mg/L (nominal); > 0.117 mg/L (meas. (geom. mean))

Additional information

One experimental study is available investigating the short-term effects of 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooct-1-ene (CAS 25291-17-2) to freshwater fish (Daikin Industries, 2007). The study was performed according to OECD 203 (GLP) using Oryzias latipes as test organism. Due to the high vapour pressure of the substance (4640 Pa at 25 °C) it was ensured during preparation of test solutions that the substance does not evaporate from the test medium by avoiding any headspace in the Erlenmeyer flasks and the test vessels. The test solution (limit concentration of 100 mg/L) was prepared by adding an appropriate amount of test substance to dilution water followed by 48 h of stirring and a settling period of 1 h. The middle layer was used for testing. Test solutions were renewed every 24 h. To verify the exposure concentrations analytical measurements using GC/MS were applied. After 96 h no mortality was observed resulting in a LC50 of > 100 mg/L (nominal) and > 0.117 mg/L (meas. (geom. mean)).