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EC number: 276-012-7 | CAS number: 71786-55-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From September 24th to October 02nd, 1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 4 April 1984
- GLP compliance:
- yes
- Details on test solutions:
- The test solutions were prepared by adding the test material directly to the test water.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source: ARA Rhein, Pratteln, Switzerland.
- Treatment: the sludge is centrifuged and washed three times with drinking water, then suspended in water with a solids content of 4 g/l and after the
addition of 20 ml synthetic sewage per litre sludge suspension aerated overnight at 20 °C. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- Nominal and measured concentrations:
- 18, 32, 56, 100, 180, 560, 1000 and 1800 mg/l, nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 ml glass beaker with 500 ml test solution.
- Aeration: air flow 0.5 to 1 l/min.
- No. of vessels per control: 2 replicates (one at the beginning and one at the end of the test).
- Sludge concentration: 1.6 g/l
TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: drinking water with a total hardness of about 180 mg CaCO3/l and pH 7.6 to 8.0.
MEASURING APPARATUS
500 ml flat bottomed flask, totally filled with test solution.
EFFECT PARAMETERS MEASURED
The percentage inhibition of the respiration was plotted against concentration on logarithmic probability paper. The EC50, EC20 and EC80 were calculated with normal statistical procedures (Regression line). - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3 854.7 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- EC20 (3h): 122.8 mg/l
- Results with reference substance (positive control):
- EC50 (3h): 5 - 5.8 mg/l
- Conclusions:
- EC50 (3h): 3854.7 mg/l (graphically determined, based on nominal conc).
- Executive summary:
The bacterial toxicity potential of the substance was investigated in respiration inhibition test. The test was conducted in accordance with the OECD Guideline for Testing of Chemicals, No. 209 (Activated Sludge, Respination Inhibition Test). Activated sludge of a predominantly domestic sewage were used. The percentage inhibition of the respiration was plotted against concentration on logarithmic probability paper. The EC50, EC20 and EC80 were calculated with normal statistical procedures (Regression line).
After the exposure period of 3 hours, the EC20 was estimated to be 122.8 mg/l, while the EC50 was estimated to be 3854.7 mg/l.
Conclusion
EC50 (3h): 3854.7 mg/l (graphically determined, based on nominal conc).
Reference
Rate of respiration inhibition
Concentration (mg/l) | Inhibition (%) | Mean value from |
18 | 11.7 | 1 test |
32 | 15.1 | 1 test |
56 | 10.3 | 1 test |
100 | 21.9 | 2 tests |
180 | 14.1 | 1 test |
560 | 34.4 | 2 tests |
1000 | 39.9 | 3 tests |
1800 | 45.1 | 2 tests |
The oxygen rates of the controls in the four test serie were between 0.74 and 1.5 mg O2/l/min.
Calculation of the regression line
Y (inhibition %) | Probits | X (conc in mg/l) | log X |
11.7 | 3.8099 | 18 | 1.2552 |
15.1 | 3.9678 | 32 | 1.5051 |
10.3 | 3.7354 | 56 | 1.7482 |
21.9 | 4.2244 | 100 | 2 |
14.1 | 3.9242 | 180 | 2.2552 |
34.4 | 4.5984 | 560 | 2.7482 |
39.9 | 4.7233 | 1000 | 3 |
45.1 | 4.8769 | 1800 | 3.2552 |
Description of key information
EC50 (3h): 3854.7 mg/l (graphically determined, based on nominal conc).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 3 854.7 mg/L
Additional information
The bacterial toxicity potential of the substance was investigated in respiration inhibition test, in accordance with the OECD Guideline 209. Activated sludge of a predominantly domestic sewage were used. The percentage inhibition of the respiration was plotted against concentration on logarithmic probability paper. The EC50, EC20 and EC80 were calculated with normal statistical procedures (Regression line).
After the exposure period of 3 hours, the EC20 was estimated to be 122.8 mg/l, while the EC50 was estimated to be 3854.7 mg/l.
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