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Diss Factsheets
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EC number: 229-158-0 | CAS number: 6420-33-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 March 2017 to 05 April 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Principles of method if other than guideline:
- Limit test based on the outcome of the acute daphnia test and lemna test according to the threshold approach as indicated in the REACH guidance
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- From control and 100 mg/L at 0, 24, 48 and 72 hours from fresh media, and at 24, 48, 72 and 96 hours from old media
Samples were frozen before analyses. After thawing the 2 mL samples were diluted with medium (factor 10). - Vehicle:
- no
- Details on test solutions:
- The test water is dechlorinated and softened tap water (analytical details are provided in the report and no contaminants that would affect the integrity and outcome of the study were found)
A nominal amount of substance (2000 mg) was dissolved in a 2000 mL volume of test water with the aid of sonication for 10 to 15 minutes to give a 1000 mg/L stock solution.
The stock solution was inverted several times to ensure adequate mixing and homogeneity. An aliquot (2000 mL) of the stock solution was dissolved in 20 liters of test water to produce the 100 mg/L test concentration. The test concentration was stirred with a flat bladed mixer
for approximately 1 minute to ensure homogeneity of the solution. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout (Oncorhynchus mykiss)
- Source: Brow Well Fisheries Limited, Hebden, near Skipton, Yorkshire, UK
- Age at study initiation: juveniles (from stock maintained in-house since January 6 2017)
- Length at study end: 6.3 cm (sd = 0.2)
- Weight at study end: 2.11 g (sd = 0.24)
- Maintenance of the stock fish: in a glass fiber tank with a "single pass" water renewal system
ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions: same as test conditions ( at 14 -15 °C ; dissolved oxygen content ≥ 9.3 mg O2/L.
- Type of food during acclimation: commercial trout pellets
- Feeding frequency during acclimation: no data
- Health during acclimation: no mortality
FEEDING DURING TEST: none (witheld from 24 h prior to exposure start - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 140 mg/L as CaCO3
- Test temperature:
- 14-15 °C
- pH:
- 7.5-7.9
- Dissolved oxygen:
- 10.6-10.9 mg/L in fresh solutions (104-108% of saturation)
8.7-9.2 mg/L in old solutions (85-98% of saturation) - Salinity:
- NA
- Nominal and measured concentrations:
- nominal: 0 and 100 mg/L
measured: at 0 mg/L < LOQ; at 100 mg/L 101-105% of nominal (in fresh solutions at 0 and 72 hours and in old solutions at 24 and 96 hours) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 25-30 L glass aquaria with 20 L test medium
- Type: closed
- Aeration: yes via narrow bore glass tubes
- Renewal rate of test solution: every 24 hours
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): NA
- Biomass loading rate: 0.74 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated and softened tap water
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 18 h light/ 6 h dark
- Light intensity: NA
EFFECT PARAMETERS MEASURED : mortalities and sub-lethal effects of exposure were recorded at 1, 3, 6, 24, 48, 72 and 96 hours after the start of exposure
TEST CONCENTRATIONS
- Range finding study: NA
- Test concentrations: 0 and 100 mg/L
- Results used to determine the concentrations for the definitive study: limit test based on the outcome of the acute dapnia test and lemna test according to the threshold approach as indicated in the REACH guidance
VALIDITY CRITERIA:
- in the control, not more than one of the fish should die or show signs of stress during the 96 hours.
- the dissolved oxygen concentration at the end of the test should be greater than or equal to 60% of Air Saturation Values in the control and test vessels. - Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other:
- Remarks:
- measured concentrations were within 80-120% of nominal
- Details on results:
- No mortality or abnormal behaviour was observed at 0 and 100 mg/L at any of the observation times.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 of the substance is > 100 mg/L
- Executive summary:
In a test according to OECD 203 (semi static with renewal every 24 hours), 7 rainbow trouts/concentration were exposed during 96 hours to 0 and 100 mg/L of the substance. The measured concentrations were between 101 -105% of nominal. No mortality or abnormal behaviour was observed at 1, 3, 6, 24, 48, 72 and 96 hours after the start of exposure in controls and at 100 mg/L. The LC50 is therefore > 100 mg/L.
Reference
Description of key information
In a test according to OECD 203 (semi static with renewal every 24 hours), 7 rainbow trouts/concentration were exposed during 96 hours to 0 and 100 mg/L of the substance. The measured concentrations were between 101 -105% of nominal. No mortality or abnormal behaviour was observed at 1, 3, 6, 24, 48, 72 and 96 hours after the start of exposure in controls and at 100 mg/L. The LC50 is therefore > 100 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.