Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 285-378-7 | CAS number: 85085-49-0 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Mentha citrata, Labiatae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Short- term toxicity to aquatic invertebrate:
Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of aquatic toxicity of the test chemical Mentha citrata, ext. (85085 -49 -0).The studies are as mentioned below:
1.Short term toxicity test was performed according to OECD 202 for test chemical on Daphnia magna Straus 1820 was evaluated for 48 h
>24 h old Daphnids were used as 5 per replicates and 4 replicates per treatment. The test was carried out in static without aeration in the dark; at 20±1°C in beakers covered with glass watches and containing 100 mL of reconstituted water of hardness 240 mg/L (CaCO3). The nominal concentrations subjected to the test were 10, 18, 32, 58 and 100 mg/L.
The effect concentration (EC50) of test chemical on Daphnia magna was observed to be 15 mg/l (95% CI: 13-17 mg/L).
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.
2.The test material was subjected to evaluate its efficiency for short term toxicity of aquatic invertebrates for 48 h according to OECD 202.
Daphnia magna from CIBA-GEIGY's own testing facility culture were used for the test. Daphnids were inoculated in glass vessels containing approx. 2.5 l daphnid medium. Water was renewed 3 times weekly. At each renewal the daphnids were fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of Tetramin extract in such quantities that the feed is consumed within 24 h.
24 h before test begin reproductive daphnia are separated from the young by sieving through a 0.8-mm sieve.
For each concentration and for the control 20 daphnids were used, in 4 replicates of 5 daphnids each. During the exposure the daphnids were not fed.Glass beakers were filled with 100 ml daphnid medium that had been aerated for 24 h before the test and covered with watch glasses during the test. The temperature was kept at20 +/- 1 °C during the test, there was no lighting in the test room and no aeration of the vessels during the test.Stock solution.250 mg test material was dissolved in and made up with daphnid medium to 2000 ml.
Nominal test concentrations were 100, 58, 32, 18 and 10 mg/l. Controls were blanks (daphnid medium only). EC50 values were calculated according to the maximum likelihood probit model
Immobilisation after 24 h was found in 17/20 daphnids and after 48 h in 19/20 daphnids. The 48-h EC50 was calculated to be 59 mg/l(53-65 mg/l, 95% CL).
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.
Thus, based on the above summarised studies, Mentha citrata, ext and it’s structurally and functionallysimilar read across substance, it can be concluded that effect concetration value is in the range 15 - 49 mg/L. Thus, comparing this value with the criteria of CLP regulation, Mentha citrata, ext can be classified as " Acute aquatic 3" for short term toxicity for aquatic invertebrate .
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate and can be categorize as "Not classified" as per CLP.
Toxicity to aquatic algae and cyanobacteria:
Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of aquatic algae and cyanobacteria of the test chemical Mentha citrata, ext. (85085 -49 -0).The studies are as mentioned below:
1. The growth inhibition test of test chemical to green algae (Scenedesmus subspicatus) was evaluated for 72 h according to OECD 201.The algae were used as 3 replicates in treatment and 6 replicates in control with the initial cell density of 0.93 x 104 cells/mL.The test was conducted in 100 mL flasks containing 50 mL of algal medium; temperature: 23±1°C; continuous illumination (8000 lux).The nominal concentrations used in the medium were 4.4, 9.6, 21, 46 and 100 mg/L. The effect of test chemical on algae was observed under two parameter mean cell density (biomass) and inhibition % (growth rate
The EbC50 (biomass) was observed to be 16 mg/l and the ErC50 ( growth rate) was observed to be 62 mg/l.Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic algae and cyanobacteria.
2.Test for determination of the inhibitory effect of substances in water on green algae Scenedesmus subspicatus was performed .
The test material was emulsified in tween 80. Scendesmus subspicatus algae were exposed to aqueous dilutions of this emulsified test substance for 96 hours in quadruplicate.The nominal test concentrations were 0 (water controls), 10, 32, 100, 320 and 1000 mg/l.
Cell densities were measured by chlorophyll fluorescence using impulse fluorometry in relative units, biomass and growth rates respectively the inhibition caused by the test substance were determined.
The effect concentration of test material on algal biomass was observed to be EbC50 = 88.3 mg/l and the effect concentration for growth rate of algae was ErC50 = 156.7 mg/l.
Thus, based on the above summarised studies, Mentha citrata, ext and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range 62 -156.7 mg/L. Thus, comparing this value with the criteria of CLP regulation, Mentha citrata, ext can be classified as " Acute aquatic 3" for short term toxicity for aquatic invertebrate .
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate and can be categorize as "Not classified" as per CLP.
Additional information
Short- term toxicity to aquatic invertebrate:
Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of aquatic toxicity of the test chemical Mentha citrata, ext. (85085 -49 -0).The studies are as mentioned below:
1.Short term toxicity test was performed according to OECD 202 for test chemical on Daphnia magna Straus 1820 was evaluated for 48 h
>24 h old Daphnids were used as 5 per replicates and 4 replicates per treatment. The test was carried out in static without aeration in the dark; at 20±1°C in beakers covered with glass watches and containing 100 mL of reconstituted water of hardness 240 mg/L (CaCO3). The nominal concentrations subjected to the test were 10, 18, 32, 58 and 100 mg/L.
The effect concentration (EC50) of test chemical on Daphnia magna was observed to be 15 mg/l (95% CI: 13-17 mg/L).
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.
2.The test material was subjected to evaluate its efficiency for short term toxicity of aquatic invertebrates for 48 h according to OECD 202.
Daphnia magna from CIBA-GEIGY's own testing facility culture were used for the test. Daphnids were inoculated in glass vessels containing approx. 2.5 l daphnid medium. Water was renewed 3 times weekly. At each renewal the daphnids were fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of Tetramin extract in such quantities that the feed is consumed within 24 h.
24 h before test begin reproductive daphnia are separated from the young by sieving through a 0.8-mm sieve.
For each concentration and for the control 20 daphnids were used, in 4 replicates of 5 daphnids each. During the exposure the daphnids were not fed.Glass beakers were filled with 100 ml daphnid medium that had been aerated for 24 h before the test and covered with watch glasses during the test. The temperature was kept at20 +/- 1 °C during the test, there was no lighting in the test room and no aeration of the vessels during the test.Stock solution.250 mg test material was dissolved in and made up with daphnid medium to 2000 ml.
Nominal test concentrations were 100, 58, 32, 18 and 10 mg/l. Controls were blanks (daphnid medium only). EC50 values were calculated according to the maximum likelihood probit model
Immobilisation after 24 h was found in 17/20 daphnids and after 48 h in 19/20 daphnids. The 48-h EC50 was calculated to be 59 mg/l(53-65 mg/l, 95% CL).
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate.
Thus, based on the above summarised studies, Mentha citrata, ext and it’s structurally and functionallysimilar read across substance, it can be concluded that effect concetration value is in the range 15 - 49 mg/L. Thus, comparing this value with the criteria of CLP regulation, Mentha citrata, ext can be classified as " Acute aquatic 3" for short term toxicity for aquatic invertebrate .
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate and can be categorize as "Not classified" as per CLP.
Toxicity to aquatic algae and cyanobacteria:
Data available for the structurally and functionally similar read across chemicals has been reviewed todetermine the short term toxicity of aquatic algae and cyanobacteria of the test chemical Mentha citrata, ext. (85085 -49 -0).The studies are as mentioned below:
1. The growth inhibition test of test chemical to green algae (Scenedesmus subspicatus) was evaluated for 72 h according to OECD 201.The algae were used as 3 replicates in treatment and 6 replicates in control with the initial cell density of 0.93 x 104 cells/mL.The test was conducted in 100 mL flasks containing 50 mL of algal medium; temperature: 23±1°C; continuous illumination (8000 lux).The nominal concentrations used in the medium were 4.4, 9.6, 21, 46 and 100 mg/L. The effect of test chemical on algae was observed under two parameter mean cell density (biomass) and inhibition % (growth rate
The EbC50 (biomass) was observed to be 16 mg/l and the ErC50 ( growth rate) was observed to be 62 mg/l.Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic algae and cyanobacteria.
2.Test for determination of the inhibitory effect of substances in water on green algae Scenedesmus subspicatus was performed .
The test material was emulsified in tween 80. Scendesmus subspicatus algae were exposed to aqueous dilutions of this emulsified test substance for 96 hours in quadruplicate.The nominal test concentrations were 0 (water controls), 10, 32, 100, 320 and 1000 mg/l.
Cell densities were measured by chlorophyll fluorescence using impulse fluorometry in relative units, biomass and growth rates respectively the inhibition caused by the test substance were determined.
The effect concentration of test material on algal biomass was observed to be EbC50 = 88.3 mg/l and the effect concentration for growth rate of algae was ErC50 = 156.7 mg/l.
Thus, based on the above summarised studies, Mentha citrata, ext and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range 62 -156.7 mg/L. Thus, comparing this value with the criteria of CLP regulation, Mentha citrata, ext can be classified as " Acute aquatic 3" for short term toxicity for aquatic invertebrate .
Even though the above effect concentration is considered as toxic, but test material is readily degradable in aquatic environment . Hence, it is considered to be non toxic to aquatic invertebrate and can be categorize as "Not classified" as per CLP.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.