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EC number: 232-577-1 | CAS number: 9001-05-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 August 2010 to 07 October 2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to the OECD Guideline 203 but is not in compliance with GLP.
- Justification for type of information:
- For industrial enzymes, the PNEC (predicted no effect concentration) value for enzymes in general is considered the highest dose tested for a given endpoint in studies conducted and published by the enzyme industry. The one exception is for proteases and oxidoreductases, where effects may be observed at lower concentrations. Similarly, although the acute fish study with catalase was not conducted GLP, the 96-hour median lethal concentration (LC50) for catalase was greater than the limit dose of 100 mg/L. The weight-of-evidence indicates that enzymes in general are not ecotoxic.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Remarks:
- See 'Justification for type of information'
- Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- (dilution in water)
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- Experimental Organism
- Source: Stillmeadow Inc.
- Age ans size: Juvenile; Range finding: 0.0133-0.02659 g and 11-13 mm; Limit: 0.0160-0.0455 g and 11-15 mm
- Quantity: Range finding: 10 per test concentration and control; Limit: 30 per test concentration and control. The organisms were randomized into containers containing the appropriate concentration of test substance
Organism Husbandry
- Test Chambers: Range finding: 1 L glass beakers filled with 1000 mL of the appropriate solution. Limit: 1 L glass beakers filled with 1000 mL of the appropriate solution
- Loading: Maximum loading of 1.0 g fish/liter
- Holding: All fish were held in the laboratory at least 14 days before they were used for testing. The fish were held in water of the quality used in the test for at least seven days immediately before testing. No signs of disease, injury, stress or mortality were observed during acclimation.
Environmental Controls:
- Set to Maintain: Temperature Range of 23 ± 2°C; 16-hours light/8-hours dark cycle
- Actual Temperature: 21-24°C
- Food: Fish were fed daily until 48 hours before the test was dosed. Fish were not fed during the test. - Test type:
- static
- Water media type:
- other: moderately hard synthetic freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Test temperature:
- 24°C
- pH:
- 7.8
- Dissolved oxygen:
- 94% in 0 mg/L 95% in 100 mg/L
- Conductivity:
- 311 µmhos/cm in 0 mg/L, 352 µmhos/cm in 100 mg/L
- Nominal and measured concentrations:
- Nominal: 0 and 100 mg/L (equivalent to 3.48 mg active enzyme protein/L)
- Details on test conditions:
- Range-finding Test: A preliminary range-finding test was conducted using concentrations of 0.1, 1.0, 10, 50, and 100 mg/L of the test substance. Following randomization, ten organisms were placed into each test beaker containing the appropriate concentration of test substance. Ten organisms, which were not exposed to test substance, served as controls to demonstrate the condition of the test population. At 0, 24, 48, 72, and 96 hours following dosing, each container was examined for mortality and the number of live fish was recorded.
Limit Test: A limit test was conducted using a concentration of 100 mg/L of the test substance. The test level consisted of three replicates often fish. Three replicates containing ten fish each were not exposed to test substance and served as controls to demonstrate the condition of the test population. At 0,6,24,48, 72 and 96 hours following dosing, each of the aquaria was examined for mortality and the number of live fish was recorded.
Chemical and Physical Monitoring: The following measurements were recorded daily during testing: dissolved oxygen, temperature, conductivity and pH of control and treated containers. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: no effetcs observed
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 3.48 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: active enzyme protein
- Basis for effect:
- other: no effects observed
- Details on results:
- A 73% survival rate was observed in fish treated with 100 mg/L of the test substance. A survival rate of 93% was observed in the control fish respectively. The 96-hour median lethal concentration (LC50) of the test substance was greater than 100 mg/L.
- Sublethal observations / clinical signs:
Limit test:
Concentration (mg/L)
Replicate
Number of Surviving Organisms
0 Hours
6 Hours
24 Hours
48 Hours
72 Hours
96 Hours
Survival (%)
0
A
10
10
10
10
10
10
93
B
10
10
10
10
9
9
C
10
10
10
10
10***
9
100
A
10
10
9*
5**
4
2
73
B
10
10
10
10
10
10
C
10
10
10
10
10
10
*One organism found dead with fibrous growth covering all of its body.
**Organisms found dead with fibrous growth covering all of their body
***One organism looks lethargic
Chemical and Physical Monitoring Data :
Table 1. Temperature (degree Celsius)
Concentration (mg/L)
Limit Test
0 hours
6 Hours
24 Hours
48 Hours
72 Hours
96 Hours
0
24
21+
21+
21+
21+
21+
100
24
+ -Temperature taken from chamber thermometer
Table2. pH
Concentration (mg/L)
Limit Test
0 hours
6 Hours
24 Hours
48 Hours
72 Hours
96 Hours
0
7,8
7,4
7,5
7,7
7,4
7,7
100
7,8
7,4
7,6
7,5
7,2
7,6
Table 3. Dissolved Oxygen (%)
Concentration (mg/L)
Limit Test
0 hours
6 Hours
24 Hours
48 Hours
72 Hours
96 Hours
0
94
86
85
83
87
86
100
95
86
85
80
81
81
Table 4. Conductivity (µmhos/cm)
Concentration (mg/L)
Limit Test
0 hours
6 Hours
24 Hours
48 Hours
72 Hours
96 Hours
0
311
309
301
317
318
317
100
352
351
346
378
357
369
- Validity criteria fulfilled:
- yes
- Remarks:
- (in both range finding and limit test control mortality did not exceed 10%)
- Conclusions:
- The 96-hour median lethal concentration (LC50) of Catalase was greater than 100 mg/L corresponding to 3.48 mg active enzyme protein/L.
- Executive summary:
The study was conducted to assess the toxicity of Catalase to the fathead minnow (Pimephales promelas) in a 96-hour test according to the OECD Guideline 203.
Test concentrations were determined by preliminary range-finding test.A test substance concentration of 100 mg/L was administered to the test system, Pimephales promelas, in moderately hard synthetic freshwater. Three replicates of ten organisms were treated with each concentration of the test substance. A control group containing thirty organisms was not exposed to test substance. Dissolved oxygen, temperature, conductivity and pH measurements were recorded at dosing and daily through study termination. Observations of mortality were made at 0, 6, 24, 48, 72 and 96 hours after treatment. The test was terminated after 96±1 hours of exposure.
The 96-hour median lethal concentration (LC50) of the test substance was greater than 100 mg/L corresponding to 3.48 mg active enzyme protein/L.
Reference
Description of key information
Under the conditions of the test, Catalase was not found to be acutely toxic to the fathead minnow (Pimephales promelas). Consequently, the 96-hour LC50 value for Catalase could not be calculated and must be >100 mg/L corresponding to 3.48 mg aep (active enzyme protein)/L.
Key value for chemical safety assessment
Additional information
The study was conducted to assess the toxicity of Catalase to the fathead minnow (Pimephales promelas) in a 96-hour test according to the OECD Guideline 203. Test concentrations were determined by preliminary range-finding test.A test substance concentration of 100 mg/L was administered to the test system, Pimephales promelas, in moderately hard synthetic freshwater. Three replicates of ten organisms were treated with each concentration of the test substance. A control group containing thirty organisms was not exposed to test substance. Dissolved oxygen, temperature, conductivity and pH measurements were recorded at dosing and daily through study termination. Observations of mortality were made at 0, 6, 24, 48, 72 and 96 hours after treatment. The test was terminated after 96±1 hours of exposure. The 96-hour median lethal concentration (LC50) of the test substance was greater than 100 mg/L corresponding to 3.48 mg active enzyme protein/L.
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