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EC number: 236-114-4 | CAS number: 13171-00-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 December 1986 to 1 January 1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: See remarks:
- Remarks:
- Guideline study without detailed information and with restrictions, e.g. inoculum was derived from only three sites instead of ten and the test was performed at 20 oC instead of 25 oC. In addition, no information was provided on test solution preparation, the amount of bottles used, the volume of the bottles used and limited information is presented on the results. Furthermore it should be noted that the substance is tested above the water solubility which may have affected the results. Nonetheless, the result is sufficiently acceptable for assessment.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- yes
- Remarks:
- e.g. inoculum was derived from only three sites and the test was performed at 20 oC, no information was provided on test solution preparation, the amount of bottles used, the volume of the bottles used and limited information is presented on the results.
- GLP compliance:
- not specified
- Oxygen conditions:
- not specified
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Activated sludge is prepared by mixing:
- sludge from the biological wast water treatment plant of the City of Geneva (Aire).
- sludge from the biological wate water treatment plant of Vernier-Ouest, Geneva.
- soil sampled on the bank of the Rhone river.
- Activated Sludge Concentration: 30 mg/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 102 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Test temperature: 20 oC
MEASURING EQUIPMENT
- Volumetric BOD Measurement, VOITH SAPROMAT, Laborapparate AG, CH-9105 Schonengrund
- TOC DOHRMANN DC 90, Schmidlin Labor + Service AG, CH-6300 Zug
ANALYTICAL PROCEDURES
- Analytical conditions of the SAPROMAT:
Closed sytem oxygen consumption measuring apparatus.
Measurement of the oxygen consumption of microorganisms using an electro-chemical analysis process.
- Measurement of the Total Organic Carbon (TOC)
High temperature catalytic combustion, detection of the carbon dioxide formed by IR.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes - Reference substance:
- aniline
- Remarks:
- 90 mg/L, purity: 99.5%
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Results as presented in the BOD curves, see included graph.
Biochemical Oxygen Demand of the test chemical measured on the BOD curve: 13 mg
Oxygen consumption of basal culture medium to which the inoculum is added, measured on the BOD curve: 19 mg
Theoretical Oxygen Demand required when the test chemical is completely oxidised: 301 mg
Degradation by Biochemical Oxygen Demand of the test chemical: 0%
The substance is not biodegradable under the test conditions.
According to the authors of the report the measure of the biodegradability can be stronly affected by the low solubility of the substance. The low value of the biodegradability observed for the test substance may be due to the low bioavailability of the product and/or its limited accessibility to the enzymatic sites responsible for the biodegradation. - Results with reference substance:
- No biodegradation percentages for the reference substance are reported. However based on the graph presented in the report and by calculating the Theoretical Oxygen Demand of the reference sustance a rough estimate for the biodegradation percentage can be calculated of ca. 65% and 70% for day 7 and 14, respectively.
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The substance showed no biodegradation under the conditions of the modified MITI test (OECD TG 301C) and should therefore not be classified as readily biodegradable.
- Executive summary:
The ready biodegradability of the test substance was investigated in a study conducted in accordance with OECD TG 301C (modified MITI test), reported without detailed information and tested with some restrictions, Reliability 2. The concentration tested was 102 mg/L test substance, with an activated sludge concentration of 30 mg/L. No biodegradation was observed for the test substance. The substance has been tested above its water solubility. Lower concentrations, however, would not have resulted in ready biodegradability in absence of obvious biodegradable groups. Based on the results of this study the substance is considered not readily biodegradable.
Reference
Description of key information
The substance showed no biodegradation under the conditions of the modified MITI test (OECD TG 301C) and should therefore not be classified as readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Key study:
The ready biodegradability of the test substance was investigated in a study conducted in accordance with OECD TG 301C (modified MITI test), reported without detailed information and tested with some restrictions, Reliability 2. The concentration tested was 102 mg/L test substance, with an activated sludge concentration of 30 mg/L. No biodegradation was observed for the test substance. The substance has been tested above its water solubility. Lower concentrations, however, would not have resulted in ready biodegradability in absence of obvious biodegradable groups. Based on the results of this study the substance is considered not readily biodegradable.
Additional available information:
Additional information is available in the RIFM database (Research Institute for Fragrance Materials) from an inherent biodegradability study (Givaudan, 1995) using the Respirometric Method (Modified MITI test II) performed in compliance with OECD TG 302C. At 30 mg/L test substance, no biodegradation was seen after 28 days. Under the conditions of this study the substance was not inherently biodegradable. Note that this study was also tested above the water solubility of the substance.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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