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EC number: 288-538-4 | CAS number: 85750-13-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Data available for the structurally similar read across chemicals has been reviewed to determine the acute oral toxicity of the test chemical 4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline(85750-13-6).The studies are as mentioned below:
1.Acute oral toxicity study was done in mouse using test chemical. No mortality was observed at dose 10000 mg/kg bw.Hence,LD50 was considered to be >10000 mg/kg body weight,when mouse was treated with test chemical orally.
2.Acute oral toxicity study was done in 5 male and 5 female Sprague Dawley using test chemical. The test material dissolved in water and administered by oral gavage route in dose concentration 2000 mg /kg bw. The dose was selected on the basis of preliminary study. All the animals were observed 1, 2and 4 hours after dosing and thereafter daily for 14 days.Body weights were recorded on days 1, 8 and 15 of the study. Macroscopic examination of main organs was performed after autopsy. No histological examinations were performed.No mortality was observed at dose 2000mg/kg bw.The only clinical sign was a pink discoloration of the skin, apparent from 1 hour to 7 days after dosing.Body weight gain was considered normal for the age and strain of rat. At autopsy an orange coloration of the mammary tissue and /or abdominal fat, attributed to the staining properties of the substance and not considered to be a toxic effect. The distribution and persistence of staining indicates that the substance has the potential to accumulate, at least at the high dose used in this acute study.Hence,LD50 was considered to be>2000mg/kg body weight. When male and female rats were treated with test chemical orally.
Thus, based on the above summarised studies,4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline(85750-13-6) and it’s structurally similar read across substance, it can be concluded that LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation,4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline(85750-13-6)cannot be classified for acute oral toxicity.Hence,based on the data available for the structurally similar read across, test chemical 4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline(85750-13-6)is not likely to be toxic atleast at the dose of >10000 mg/Kg bw.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- experimental data of read across substances
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two acute oral toxicity studies as-
1.and 2. Acute Oral toxicity test was carried out to study the effects of the test chemicals on rodents - GLP compliance:
- not specified
- Test type:
- other: no data available
- Limit test:
- no
- Species:
- other: 1. mouse 2. rat
- Strain:
- other: 1.not specified 2.Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- No data available
- Route of administration:
- other: 1. not specified 2.oral: gavage
- Vehicle:
- other: 1.not specified 2.water
- Details on oral exposure:
- No data available
- Doses:
- 1.10000 mg/kg bw
2.2000 mg/kg bw - No. of animals per sex per dose:
- 1.no data available
2.Total:10
Male :5
Female:5 - Control animals:
- not specified
- Details on study design:
- 1. No data available
2.Details on study design
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: The animals were observed 1, 2and 4 hours after dosing and thereafter daily for 14 days.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, organ weights were observed. Histopathology: No histological examinations were performed. - Statistics:
- No data available
- Preliminary study:
- 1.No data available
2.The dose group was selected on the basis of a preliminary range-finding study in which rats were given the test compound in water at dose levels from 100 to 2000 mg/kg bw, one death was reported at 1000 mg/kg bw but the only reported clinical signs were dose-related pink skin tone due to the compound and the death was considered to be unrelated to treatment - Sex:
- not specified
- Dose descriptor:
- LD50
- Effect level:
- > 10 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: no mortality was observed
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: no mortality was observed
- Mortality:
- 1.No mortality was observed at dose 10000 mg/kg bw
2.No mortality was observed at dose 2000 mg/kg bw - Clinical signs:
- 1.No data available
2. The only clinical sign was a pink discoloration of the skin, apparent from 1 hour to 7 days after dosing. - Body weight:
- 1.No data available
2.Body weight gain was considered normal for the age and strain of rat. - Gross pathology:
- 1.No data available
2.At autopsy an orange coloration of the mammary tissue and /or abdominal fat, attributed to the staining properties of the substance and not considered to be a toxic effect - Other findings:
- 1.No data available
2.The distribution and persistence of staining indicates that the substance has the potential to accumulate, at least at the high dose used in this acute study - Interpretation of results:
- other: not classified
- Conclusions:
- The test chemical 4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline (85750-13-6) is not likely to be toxic atleast in the dose of >10000 mg/Kg bw.
- Executive summary:
Data available for the structurally similar read across chemicals has been reviewed to determine the acute oral toxicity of the test chemical 4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline(85750-13-6).The studies are as mentioned below:
1.Acute oral toxicity study was done in mouse using test chemical. No mortality was observed at dose 10000 mg/kg bw.Hence,LD50 was considered to be >10000 mg/kg body weight,when mouse was treated with test chemical orally.
2.Acute oral toxicity study was done in 5 male and 5 female Sprague Dawley using test chemical. The test material dissolved in water and administered by oral gavage route in dose concentration 2000 mg /kg bw. The dose was selected on the basis of preliminary study. All the animals were observed 1, 2and 4 hours after dosing and thereafter daily for 14 days.Body weights were recorded on days 1, 8 and 15 of the study. Macroscopic examination of main organs was performed after autopsy. No histological examinations were performed.No mortality was observed at dose 2000mg/kg bw.The only clinical sign was a pink discoloration of the skin, apparent from 1 hour to 7 days after dosing.Body weight gain was considered normal for the age and strain of rat. At autopsy an orange coloration of the mammary tissue and /or abdominal fat, attributed to the staining properties of the substance and not considered to be a toxic effect. The distribution and persistence of staining indicates that the substance has the potential to accumulate, at least at the high dose used in this acute study.Hence,LD50 was considered to be>2000mg/kg body weight. When male and female rats were treated with test chemical orally.
Thus, based on the above summarised studies,4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline(85750-13-6) and it’s structurally similar read across substance, it can be concluded that LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation,4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline(85750-13-6)cannot be classified for acute oral toxicity.Hence,based on the data available for the structurally similar read across, test chemical 4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline(85750-13-6)is not likely to be toxic atleast at the dose of >10000 mg/Kg bw.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 10 000 mg/kg bw
- Quality of whole database:
- Data is Klimisch 2 and from authoritative database
Acute toxicity: via inhalation route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Data available for the structurally similar read across chemicals has been reviewed to determine the acute oral toxicity of the test chemical 4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline(85750-13-6).The studies are as mentioned below:
1.Acute oral toxicity study was done in mouse using test chemical. No mortality was observed at dose 10000 mg/kg bw.Hence,LD50 was considered to be >10000 mg/kg body weight,when mouse was treated with test chemical orally.
2.Acute oral toxicity study was done in 5 male and 5 female Sprague Dawley using test chemical. The test material dissolved in water and administered by oral gavage route in dose concentration 2000 mg /kg bw. The dose was selected on the basis of preliminary study. All the animals were observed 1, 2and 4 hours after dosing and thereafter daily for 14 days.Body weights were recorded on days 1, 8 and 15 of the study. Macroscopic examination of main organs was performed after autopsy. No histological examinations were performed.No mortality was observed at dose 2000mg/kg bw.The only clinical sign was a pink discoloration of the skin, apparent from 1 hour to 7 days after dosing.Body weight gain was considered normal for the age and strain of rat. At autopsy an orange coloration of the mammary tissue and /or abdominal fat, attributed to the staining properties of the substance and not considered to be a toxic effect. The distribution and persistence of staining indicates that the substance has the potential to accumulate, at least at the high dose used in this acute study.Hence,LD50 was considered to be>2000mg/kg body weight. When male and female rats were treated with test chemical orally.
Thus, based on the above summarised studies,4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline(85750-13-6) and it’s structurally similar read across substance, it can be concluded that LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation,4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline(85750-13-6)cannot be classified for acute oral toxicity.Hence,based on the data available for the structurally similar read across, test chemical 4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline(85750-13-6)is not likely to be toxic atleast at the dose of >10000 mg/Kg bw.
Justification for classification or non-classification
Based on the above experimental studies on 4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline(85750-13-6)and it’s structurally similar read across substances, it can be concluded that LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline(85750-13-6)cannot be classified for acute oral toxicity.
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