Reaction mass of bis[2-[2-(2-butoxyethoxy)ethoxy]ethyl]adipate and 2-[2-(2-butoxyethoxy)ethoxy]ethyl (3,6,9,12-tetraoxahexadecyl)adipate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16. Jan 2017 - 14 May 2017

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Details on preparation of test sulution missing

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

A test solution containing 101.8 mg/L in dilution water was prepared

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain/clone: Berlin
- Source: Umweltbundesamt Berlin, Germany
- Age of parental stock (mean and range, SD): <24 h

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

19.7 – 21.1 °C

pH:

7.7

Dissolved oxygen:

8.6 mg/L

Nominal and measured concentrations:

Nominal test substance concentration: 100 mg/L
Calculated test item concentration based on DOC at 0 h: 100.6 mg/L
Calculated test item concentration based on DOC at 48 h: 119.7 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Beakers, glass, nominal volume 50 mL.
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionsed water with an enrichment of certain minerals acc. to guideline


OTHER TEST CONDITIONS
- Photoperiod:16:8 h day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mobility of D. magna after 24 and 48 hours

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: none
- Mortality of control: none
- Other adverse effects control: none

No immobilised Daphnids observed.

Results with reference substance (positive control):

Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study (16102802R201). The 24h-EC50i value was determined as 1.8 mg/L, lying within the range of 0.6 – 2.1 mg/L which is mentioned in the OECD guide-line.

Description of key information

EC50 (48 h) > 100 mg/L (nominal)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

The toxicity of the target substance (EC 943-330-9) to aquatic invertebrates was investigated in a study following OECD 202 (GLP). The test organismDaphnia magnawas exposed to a nominal test substance concentration of 100 mg /L in a limit test. The test substance concentration was verified by DOC analysis at test start and test termination after 48 h. The measured concentrations were in a range of 101 % - 120 % of the nominal concentration. Therefore, the effect concentrations were based on the nominal concentrations instead of the measured concentrations. Mortality or behavioural abnormalities were not observed. An EC50 (48 h) > 100 mg/L based on the nominal concentration was reported.