Castor oil, ester with glycerol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23 Mar - 25 Mar 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions (no purity, no information on dissolved oxygen at test end)

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

adopted 1984

Deviations:

yes

Remarks:

dissolved oxygen at test end is not reported

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct dispersion of 2 g test material in 2 L of reconstituted water to give a stock solution from which dilutions were made to obtain the test series.

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

270 mg/L as CaCO3

Test temperature:

21 °C

pH:

7.8 ± 0.2

Dissolved oxygen:

Medium was air-saturated at test start.

Nominal and measured concentrations:

Nominal: 0, 10, 18, 32, 56, 100, 180, 320, 560 and 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 200 mL
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water was prepared from the following mineral salt stock solutions that were diluted 1:4 in deionised water: 11.76 g/L CaCl2*2H2O, 4.93 g/L MgSO4*7H2O, 2.59 g/L NaHCO3, 0.23 g/L KCl. The medium was aerated until oxygen saturation was reached, pH was adjusted to 7.8 ± 0.2 with NaOH or HCl if necessary.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mobility was observed after 24 h and 48 h.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx. 1.8
- Range finding study: yes
- Results used to determine the conditions for the definitive study: The range finding study indicated a 48 h NOEC and a 48 h EC50 would be obtained within the range of the concentrations chosen.

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL = 170 - 250 mg/L

Details on results:

- Mortalities in the control vessels: None
- The results of the test solution analysis showed an increasing carbon concentration with increasing nominal test concentration with the exception of the 10 mg/L test group. This erroneous value was considered to be due to sampling error and/or analytical variation. Also there was no significant change in carbon concentration observed over 48 h exposures period. The results were taken to demonstrate the stability of the test concentrations employed during the period of exposure.

Table 1: Additional information on effect concentrations

Duration	Endpoint	Effect conc.	Nom./Meas.	Conc. based on	Basis for effect	Remarks
24 h	EC50	330 mg/L	nominal	test mat.	mobility	95% CL = 270 – 410 mg/L
24 h	NOEC	56mg/L	nominal	test mat.	mobility
48 h	NOEC	56 mg/L	nominal	test mat.	mobility

Table 2: Cumulative Immobilisation Data for D. magna Exposed for 48 h to the test substance (R = Replicate)

Nominal Concentration of test substance (mg/L)	Cumulative immobilised Daphnia (initial population: 10 per replicate)
	24 h				48 h
	R1	R2	Total	%	R1	R2	Total	%
Control	0	0	0	0	0	0	0	0
10	0	0	0	0	0	0	0	0
18	0	0	0	0	0	0	0	0
32	0	0	0	0	0	0	0	0
56	0	0	0	0	0	0	0	0
100	1	1	2	10	1	2	3	15
180	2	2	4	20	5	4	9	45
320	3	4	7	35	6	7	13	65
560	8	7	15	75	10	10	20	100
1000	10	10	20	100	10	10	20	100

Table 3: Results from Total Organic Carbon (TOC) Analysis

Samples	Nominal Concentration (mg/L)	Concentration of TOC corrected for control (mg C/L)
0 h	10	17.576
	32	15.296
	100	48.266
	320	157.746
	1000	518.246
48 h	10	11.951
	32	13.181
	100	43.841
	320	147.851
	1000	490.051

Description of key information

The study was conducted according to OECD 202.

LC50 (48h) = 200 mg/L

NOEC (48h) = 56 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

200 mg/L

Additional information

The study with Castor oil, ester with glycerol (CAS 68459-67-6) was performed according to OECD 202 (GLP compliance not specified) under static conditions using the freshwater flea Daphnia magna as test organism. The daphnids were exposed to nominal concentrations of 0, 10, 18, 32, 56, 100, 180, 320, 560 and 1000 mg/L over a period of 48h. EC50 (48h) was determined to be 200 mg/L. The highest test concentration showing no adverse effects (NOEC) was 56 mg/L.