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EC number: 288-511-7 | CAS number: 85736-99-8 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 53228.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
The ready biodegradability of the test substance was investigated according to the OECD guideline 302 C and OECD guideline 301 B. Under the test conditions, the test substance was found to be not biodegradable over the test period of 28 days. Furthermore, the test substance showed no inhibitory effect on the microorganisms.
Additional information
OECD 302C:
The test item C.I. Leuco Sulphur Brown 96 was investigated for its inherent biodegradability in a manometric respirometry test over 28 days based on the OECD Guideline for Testing of Chemicals No 302 C (1981). The following modifications were made: activated sludge from one source was used, the activated sludge was not fed during the holding period of maximum seven days, the test was run at 22 °C, the test water composition was slightly changed, and only the biological oxygen demand (BOD) was monitored, no test item specific analysis was performed.
The oxygen consumption of the test item C.I. Leuco Sulphur Brown 96 in the test media slightly lower than the normal range of the oxygen consumption in the inoculum controls.
Consequently, C.I. Leuco Sulphur Brown 96 was not biodegradable under the test conditions within 28 days.
In the toxicity control, containing both the test item and the reference item sodium benzoate, C.I. Leuco Sulphur Brown 96 had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration of 224 mg/L.
In the procedure controls, the reference item (sodium benzoate) was degraded by an average of 75% and 86% by Exposure Days 7 and 14, respectively, thus confirming suitability of the activated sludge. At the end of the test (Day 28), the reference item was degraded by an average of 93%.
OECD 301B:
The test item C.I. Leuco Sulphur Brown 96 was investigated for its ready biodegradability in a 28‑Day CO2Evolution (Modified Sturm) Test according to the Method C.4-C of Commission Regulation (EC) No 440/2008, and the OECD Guideline for Testing of Chemicals, No. 301 B (1992) and the US EPA Draft Ecological Effects Test Guidelines OPPTS 835.3110 (Paragraph (m)).
The test item C.I. Leuco Sulphur Brown 96 was not biodegradable under the test conditions within 28 days.
In the toxicity control, containing both C.I. Leuco Sulphur Brown 96 and the reference item sodium benzoate, no inhibitory effect on the biodegradation of the reference item was determined. Thus, C.I. Leuco Sulphur Brown 96 had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration of 167 mg/L.
In the procedure controls, average biodegradation of the reference item was 82% by Exposure Day 14, thus confirming suitability of the activated sludge (≥60% degradation by Exposure Day 14). By the end of the test (Exposure Day 28), average biodegradation was 94%.
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