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EC number: 235-166-5 | CAS number: 12108-13-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Not mentioned
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study performed similar to OECD 473 wih minor deviations.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- Deviations:
- yes
- Remarks:
- No detailed information about test substance.
- GLP compliance:
- not specified
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- Tricarbonyl(methylcyclopentadienyl)manganese
- EC Number:
- 235-166-5
- EC Name:
- Tricarbonyl(methylcyclopentadienyl)manganese
- Cas Number:
- 12108-13-3
- Molecular formula:
- C9H7MnO3
- IUPAC Name:
- tricarbonyl(methyl-η5-cyclopentadienyl)manganese
- Details on test material:
- - Name of test material (as cited in study report): mmt
- Stability under test conditions: Stable, cells treated in dark incubators
- Test material handling: When dilutions were being prepared and aftewards added to the cells, the compound was exposed to light
Constituent 1
Method
- Target gene:
- The target is not a specific gene but the structural integrity of the genetic material expressed as chromosome aberrations visible with the light microscope.
Species / strain
- Species / strain / cell type:
- Chinese hamster Ovary (CHO)
- Details on mammalian cell type (if applicable):
- - Type and identity of media: Eagle MEM supplementedwith 1% sodium pyruvate, 1% non-essential amino acids and 10% fetal calf serum (Gibco)
- Properly maintained: yes
- Periodically checked for karyotype stability: No data
- Periodically "cleansed" against high spontaneous background: No data - Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- Exogenous Metabolic activation medium: 5.4% 20 mM HEPES buffer pH7.2, 0.2% 0.5 M MgCl2, 0.2% 3.3 KCl, 2% 40mM NADP, 2% 50 Mm Glucose-6-Phosphate and 7% Aroclor 1254-induced rat liver homogenate (S9)
- Test concentrations with justification for top dose:
- 0, 0.01, 0.02 and 0.04 µl/ml
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: the test chemical was diluted in either serum-free complete medium or an exogenous metabolic activation medium containing: 5.4% 20 mM HEPES buffer pH 7.2 (Sigma, St. Louis, MO); 0.2% 0.5 M MgCl2 (Sigma); 0.2% 3.3 M KCl; 2% 40 mM NADP; 2% 50 mM glucose-6-phosphatase and 7% Aroclor 1254-induced rat liver homogenate (S9).
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- other: No clear information available on the negative control, it is assumed to have been the vehicle control.
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Remarks:
- Test without metabolic activation
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- No clear information available on the negative control, it is assumed to have been the vehicle control.
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- cyclophosphamide
- Remarks:
- Test with metabolic activation
- Details on test system and experimental conditions:
- DURATION
- Exposure duration:
-Trial 1 (absence of metabolic activation) 3 hours; Trial 2 (absence of metabolic activation) 16 hours
-Trial 1 and 2 (presence of metabolic activation) 3 hours
NUMBER OF CELLS EVALUATED: One hundred metaphase cells were analysed from each of two cultures for each treatment. Only 50 cells were scored when the frequency of chromosomal aberrations was very high.
- Evaluation criteria:
- Chromosomal aberrations.
- Statistics:
- Data was analysed using the chromosomal aberration data management and analysis system software developed under contact to the U.S.Environmental Protection Agency.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- No additional information.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1 - Induction of chromosomal aberrations by mmt in the absence of metabolic activation
Dose (µl/ml) |
Number of cells |
Chromatid |
Chromosome |
Aberrations/cell (± Standard Error) |
% Cells with Aberrations (± Standard Error) |
||||
Gaps |
Breaks |
Exch |
Gaps |
Breaks |
Exch |
||||
Trial 1, 3 hr exposure |
|||||||||
0 |
200 |
0 |
3 |
0 |
0 |
2 |
3 |
0.040±0.000 |
4±0 |
0.01 |
200 |
1 |
2 |
0 |
2 |
2 |
5 |
0.045±0.005 |
4.5±0.5 |
0.02 |
200 |
0 |
1 |
0 |
3 |
1 |
3 |
0.025±0.015 |
2.5±1.5 |
0.04 |
90 |
2 |
0 |
0 |
0 |
2 |
2 |
0.156±0.122 |
5.56±1.5 |
MMS |
60 |
0 |
22 |
24 |
0 |
20 |
1 |
**8.583±0.483 |
**100±0 |
ρ value for trend analysis 0.088 0.412 |
|||||||||
Trial 2, continuous (16 hr) exposure |
|||||||||
0 |
191 |
4 |
0 |
0 |
4 |
4 |
9 |
0.063±0.008 |
6.28±0.75 |
0.01 |
200 |
1 |
2 |
2 |
2 |
3 |
5 |
0.060±0.020 |
6.00±2.00 |
0.02 |
200 |
1 |
4 |
0 |
3 |
7 |
13 |
*0.115±0.045 |
10.00±1.00 |
0.04 |
150 |
1 |
4 |
0 |
9 |
5 |
3 |
0.080±0.030 |
8.00±3.00 |
MMS |
45 |
1 |
45 |
32 |
2 |
31 |
4 |
**8.220±0.430 |
**100.00±0.00 |
ρ value for trend analysis 0.404 0.178 |
Table 2 - Induction of chromosomal aberrations by mmt in the presence of metabolic activation
Dose (µl/ml) |
Number of cells |
Chromatid |
Chromosome |
Aberrations/cell (± Standard Error) |
% Cells with Aberrations (± Standard Error) |
||||
Gaps |
Breaks |
Exch |
Gaps |
Breaks |
Exch |
||||
Trial 1, 3 hr exposure |
|||||||||
0 |
200 |
3 |
3 |
0 |
0 |
9 |
3 |
0.055±0.015 |
4.50±0.50 |
0.01 |
75 |
1 |
5 |
0 |
1 |
0 |
1 |
0.080±0.030 |
4.00±0.00 |
0.02 |
200 |
4 |
7 |
7 |
1 |
3 |
12 |
**0.135±0.025 |
*10.50±0.50 |
0.04 |
100 |
4 |
34 |
17 |
4 |
14 |
10 |
**1.080±0.220 |
**45.00±7.00 |
CP |
100 |
1 |
44 |
34 |
10 |
39 |
20 |
**1.450±0.270 |
**65.00±5.00 |
ρ value for trend analysis 0.000 0.000 |
|||||||||
Trial 2, 3 hr exposure |
|||||||||
0 |
200 |
1 |
1 |
1 |
0 |
2 |
11 |
0.065±0.045 |
5.00±3.00 |
0.01 |
200 |
0 |
1 |
0 |
1 |
3 |
5 |
0.045±0.005 |
4.00±1.00 |
0.02 |
200 |
1 |
11 |
7 |
2 |
13 |
8 |
*0.295±0.128 |
14.50±3.65 |
0.04 |
100 |
4 |
33 |
30 |
6 |
22 |
13 |
**1.760±0.060 |
**51.00±3.00 |
CP |
100 |
0 |
52 |
45 |
1 |
42 |
9 |
**3.110±0.190 |
**76.00±0.00 |
ρ value for trend analysis 0.000 0.000 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive with metabolic activation
negative without metabolic activation
In the presence of metabolic activation, mmt induced structural chromosomal aberrations in Chinese Hamster Ovary Cells.. There was significant (p<0.0114), reproducible increase in chromosomal aberrations at concentrations as low as 0.02 µl/ml (0.12 mM). Without metabolic activation, mmt failed to induce a significant increase in chromosomal aberrations following either a 3 hr (p = 0.412) or continuous (p = 0.178) exposure. - Executive summary:
A chromosome aberration assay was conducted similar to OECD 473 using wild-type Chinese hamster ovary (CHO) cells exposed to mmt at concentrations of 0, 0.01, 0.02 and 0.04 µl/ml with and without metabolic activation.
mmt was tested up to 0.04 µl/ml, which is within the solubility range. Positive controls induced, as expected, an increase in chromosomal aberrations both with metabolic activation (cyclophosphamide) and without metabolic activation (methylmethanesulfonate). For mmt, in absence of metabolic activation, there was no evidence of an induction of chromosomal aberration, while in the presence of metabolic activation, there was a concentration dependent positive response for the two highest concentrations tested (0.02 and 0.04 µl/ml mmt).
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