Exo-1-methyl-4-(1-methylethyl)-7-oxabicyclo[2.2.1]heptan-2-ol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987-12-15 to 1987-12-17

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(1S,2S)-1-methyl-4-(propan-2-yl)-7-oxabicyclo[2.2.1]heptan-2-ol

Cas Number:

87172-89-2

IUPAC Name:

(1S,2S)-1-methyl-4-(propan-2-yl)-7-oxabicyclo[2.2.1]heptan-2-ol

Test material form:

solid

Specific details on test material used for the study:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Following its arrival in Compound Control this test substance was stored in the dark at ambient temperature.

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 53, 95, 170, 310, 556 and 1000 mg/L
- Sampling method: Samples of water (50-60 mL) were removed from each freshly prepared test solution (t0) and at 24 h after their preparation (t24). Samples were also taken 48 h after preparation (t48).

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: The laboratory culture of D. magna is derived from a clone held at ICI's Brixham Laboratory. The ICI clone was itself obtained from the Institut National de Recherche Chimique Applique (I.R.Ch.A.), France.
- Age of parental stock (mean and range, SD): less than 24 h old

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

172 mg/L as CaCO3

Test temperature:

20.3 - 21.2 °C

pH:

8.0 - 8.2

Dissolved oxygen:

8.6 - 9.2 mg/L

Salinity:

not applicable

Conductivity:

No data

Nominal and measured concentrations:

Nominal concentrations: 53, 95, 170, 310, 556 and 1000 mg/L
Mean measured concentrations: <0.01, 56, 91, 170, 295, 515 and 925 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 150 mL crystalising dishes, filled with 100 mL aliquot of each test solution
- Aeration: The water was aerated for 24 h before use and the dissolved oxygen concentration was measured.
- Renewal rate of test solution: Test was carried out without renewal of the test solutions
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The water used for the culturing and testing of D. magna was a reconstituted fresh water prepared by dissolving the following amounts of Analar grade salts in glass-distilled deionised water
- NaHCO3: 192 mg/L
- CaSO4 * H2O: 120 mg/L
- MgSO4: 120 mg/L
- KCl: 8 mg/L
This recipe has been recommended as one suitable for producing a 'hard' water by the U.S. Environmental Protection Agency (1975).

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/ 8 h dark

EFFECT PARAMETERS MEASURED: The number of immobilised D. magna were recorded after 24 and 48 h exposure. D. magna were considered to be immobile if they did not swim during a 15 second period of observation.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

840 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% fiducial limits: 660-1300 mg/L

Details on results:

The test substance appeared to be completely soluble at all concentrations.

Reported statistics and error estimates:

The 48 h EC50 value was calculated using probit analysis after log transformation of the concentrations.

Any other information on results incl. tables

Table 1: Break-through concentration of the test item with Bond Elut C18 cartridges

Concentration of the test item added (mg/L) – 50 mL volume	Recovery (%)
200	89
300	83
400	63
500	55
1000	31

  

Table 2: Repeatability study of the test item analysis

Replicate number	% Recovery
	Concentration of the test item added (mg/L)
	10	100
(i)	89	96
(ii)	90	105
(iii)	96	99
(iv)	96	98
(v)	93	105

 

Table 3: Concentration of the test item in Daphnia magna test solutions. Fresh (t0) solutions are expressed as a percentage of the nominal concentrations. Old (t24 and t48) solutions are expressed as a percentage of the fresh solutions.

Nominal Concentration (mg/L)	Concentration of the test item in test solutions (%)
	t0	t24	t48
	(% of nominal)	(% of t0)	(% of t0)
Control	-	-	-
53	106	104	98
95	94	98	108
170	97	100	109
310	87	106	120
556	83	109	126
1000	85	107	121
Range	83-106	98-109	98-126
Mean	92	104	114

Concentration of the test item added (mg/L)	Recovery (%)
100	105, 97, 99

Table 4: Concentration of the test item in water

Nominal concentration of the test item in water (mg/L)	Determined concentration of the test item in water (mg/L)
	t0	t24	t48	Mean concentration (mg/L)
Control	<0.01	<0.01	<0.01	<0.01
53	56	58	55	56
95	89	87	96	91
170	165	165	180	170
310	270	285	325	295
556	460	500	580	515
1000	845	900	1025	925

 Table 5: Immobilisation of Daphnia magna exposed to a range of concentrations of the test item

Concentration of the test item (mg/L)	Number of D. magna	Number immobilized
		24 h	48 h
Control	10	0	0
	10	0	0
53	10	0	0
	10	0	0
95	10	0	0
	10	0	0
170	10	0	0
	10	0	0
310	10	0	1
	10	0	0
556	10	0	3
	10	2	4
1000	10	2	5
	10	5	6

 

Applicant's summary and conclusion

Conclusions:

In this 48-h immobilisation test with daphnia magna an EC50 of 840 mg/L was determined for the test item.

Executive summary:

The acute toxicity of the test item to Daphnia magna was determined in a 48 h immobilisation test. The water fleas were exposed to nominal concentrations of 53, 95, 170, 310, 556 and 1000 mg/L in a static test without renewal of test solution.

At 24 h, less than 50% of D. magna exposed to the highest concentration tested (1000 mg/L) were immobilised. The 24 h EC50 was therefore greater than 1000 mg/L. The 48 h EC50 was calculated to be 840 mg/L (95% fiducial limits 660 -1300 mg/L). The concentration of the test item in the freshly prepared test solutions ranged from 83 to 106% of nominal with a mean of 92%. After 24 and 48 h in the test vessels the concentration of the test substance in the test solutions averaged 104 and 114% of the initial concentrations, respectively.

The test substance appeared to be completely soluble at all concentrations.