Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 219-546-8 | CAS number: 2459-09-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- 96 h toxicity test of substance methyl isonicotinate was conducted in Ciliate Tetrahymena pyriformis.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (IUPAC name): Methyl Isonicotinate
- Molecular formula: C7H7NO2
- Molecular weight: 137.1373 g/mol
- Smiles notation: c1(C(=O)OC)ccncc1
- InChl: 1S/C7H7NO2/c1-10-7(9)6-2-4-8-5-3-6/h2-5H,1H3
- Substance type: Organic
- Physical state: Liquid
- Analytical purity: >95 %
- Other: The test chemicals were purchased from Aldrich Chemical Company, Wilwaukee, Wisconsin, or Pfaltz and Bauer Inc., Stamford, Connecticut, and not repurified. - Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: Test chemical conc. used for the study was 0.5 mg/l.
- Vehicle:
- not specified
- Details on test solutions:
- Methyl Isonicotinate was diluted to 500 g/l with sterile distilled water.
- Test organisms (species):
- Tetrahymena pyriformis
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 60 h
- Test temperature:
- 28°C
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- No. of vessels per concentration (replicates): Each test chemical was assayed in duplicate for a minimum of three replicates using a five-step graded concentration series.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Population growth and density were determined spectrophotometrically as absorbance at 540 mm. - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 60 h
- Dose descriptor:
- EC50
- Effect conc.:
- 893.32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Population
- Remarks on result:
- other: 95% C. I. = 701.10 - 2161.48 mg/l
- Reported statistics and error estimates:
- Population growth determinations were done using probit analysis in the Statistical Analysis System (SAS) software. For these data Y equals percentage control absorbance and X equals concentration (mg/liter). The 60-hr IGCso (50% growth inhibition concentration) and 95% confidence interval were determined and transformed to millimoles per liter for comparison. The goodness of fit was determined using the x2 value.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 60 hr EC50 value of test chemical methyl isonicotinate was determined to be 893.32 mg/l in Ciliate Tetrahymena pyriformis.
- Executive summary:
96 h toxicity test of substance methyl isonicotinate was conducted in Ciliate Tetrahymena pyriformis. Methyl Isonicotinate was diluted to 500 g/l with sterile distilled water. The study was performed in freshwater under static conditions at a temperature of 28°C.The amicronucleated strain GL-C of the common freshwater ciliate Tetrahymena pyriformis was reared in axenic culture at 28°C in a semidefined medium. Cultures were grown for 60 hr in 250-ml Erlenmeyer flasks containing 50 ml of test solution. Population growth and density were determined spectrophotometrically as absorbance at 540 mm. Each test chemical was assayed in duplicate for a minimum of three replicates using a five-step graded concentration series. Each replicate was assayed with freshly prepared stock solutions and flasks without test chemicals were used as controls.Population growth determinations were done using probit analysis in the Statistical Analysis System (SAS) software. For these data Y equals percentage control absorbance and X equals concentration (mg/liter). The 60-hr IGCso (50% growth inhibition concentration) and 95% confidence interval were determined and transformed to millimoles per liter for comparison. The goodness of fit was determined using the x2 value.The 60 hr EC50 value of test chemical methyl isonicotinate was determined to be 893.32 mg/l in Ciliate Tetrahymena pyriformis.
Reference
Table:POPULATION GROWTH INHIBITIONTO Tetrahymena of SELECTED FOUR-POSITION PYRIDINES.
Derivative |
CAS no. |
Probit regression equation |
P > x2 |
n |
60 h IGC50 (mg/l) |
OCOCH3 |
2459-09-8 |
Y = 9.2858 - 0.0048X |
0.9847 |
23 |
893.32 mg/l (701.10 - 2161.48 mg/l) |
Description of key information
96 h toxicity test of substance methyl isonicotinate was conducted in Ciliate Tetrahymena pyriformis (T. WAYNE SCHULTZ et. al; 1985). Methyl Isonicotinate was diluted to 500 g/l with sterile distilled water . The study was performed in freshwater under static conditions at a temperature of 28°C.The amicronucleated strain GL-C of the common freshwater ciliate Tetrahymena pyriformis was reared in axenic culture at 28°C in a semidefined medium. Cultures were grown for 60 hr in 250-ml Erlenmeyer flasks containing 50 ml of test solution. Population growth and density were determined spectrophotometrically as absorbance at 540 mm. Each test chemical was assayed in duplicate for a minimum of three replicates using a five-step graded concentration series. Each replicate was assayed with freshly prepared stock solutions and flasks without test chemicals were used as controls.Population growth determinations were done using probit analysis in the Statistical Analysis System (SAS) software. For these data Y equals percentage control absorbance and X equals concentration (mg/liter). The 60-hr IGCso (50% growth inhibition concentration) and 95% confidence interval were determined and transformed to millimoles per liter for comparison. The goodness of fit was determined using the x2 value.The 60 hr EC50 value of test chemical methyl isonicotinate was determined to be 893.32 mg/l in Ciliate Tetrahymena pyriformis.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 893.32 mg/L
Additional information
Various experimental studies for the target chemical Methyl Isonicotinate (CAS No. 2459-09-8)were reviewed for toxicity to micro-organism endpoint which are summarized as below:
In an experimental key study from peer reviewed journal (T. WAYNE SCHULTZ et. al; 1985), 96 h toxicity test of substance methyl isonicotinate was conducted in Ciliate Tetrahymena pyriformis. Methyl Isonicotinate was diluted to 500 g/l with sterile distilled water. The study was performed in freshwater under static conditions at a temperature of 28°C.The amicronucleated strain GL-C of the common freshwater ciliate Tetrahymena pyriformis was reared in axenic culture at 28°C in a semidefined medium. Cultures were grown for 60 hr in 250-ml Erlenmeyer flasks containing 50 ml of test solution. Population growth and density were determined spectrophotometrically as absorbance at 540 mm. Each test chemical was assayed in duplicate for a minimum of three replicates using a five-step graded concentration series. Each replicate was assayed with freshly prepared stock solutions and flasks without test chemicals were used as controls.Population growth determinations were done using probit analysis in the Statistical Analysis System (SAS) software. For these data Y equals percentage control absorbance and X equals concentration (mg/liter). The 60-hr IGCso (50% growth inhibition concentration) and 95% confidence interval were determined and transformed to millimoles per liter for comparison. The goodness of fit was determined using the x2 value. The 60 hr EC50 value of test chemical methyl isonicotinate was determined to be 893.32 mg/l in Ciliate Tetrahymena pyriformis.
Another aquatic toxicity study of micro-organism to the test substance Methyl Isonicotinate (CAS no. 2459-09-8) was conducted using Photobacterium phosphoreum, strain NRRL-B-11177 (also referred to as Vibrio fischerii, strain NRRL-B-11177) (Klaus L.E. Kaiser and Virginia S. Palabrica, 1991). The study was performed at a temperature of 15ᵒC and pH range 5 to 9, respectively. Recommneded reference substance that can be used for the study were Phenol and Sodium pentachlorophenate, respectively. When the test bacterium Photobacterium phosphoreum, strain NRRL-B-11177 was exposed to the test chemical Methyl Isonicotinate, reduction in light output was observed. Thus, based on this effect, the EC50 value during 5, 15 and 30 min exposure period was determined to be 198.235, 207.588 and 217.3669 mg/l, respectively.
Thus, based on the overall reported results for target chemical Methyl Isonicotinate, EC50 value ranges from198.235 to 893.32 mg/l, respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.