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EC number: 246-874-9 | CAS number: 25340-17-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1987
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 81-1 (Acute Oral Toxicity)
- Deviations:
- no
- Remarks:
- Not specified in report.
- Qualifier:
- according to guideline
- Guideline:
- other: FIFRA Subdivision F; TSCA
- Principles of method if other than guideline:
- Method: T03-03:EPA OPP 81-1; FIFRA Subdivision F; TSCA
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Diethylbenzene
- EC Number:
- 246-874-9
- EC Name:
- Diethylbenzene
- Cas Number:
- 25340-17-4
- Molecular formula:
- C10H14
- IUPAC Name:
- diethylbenzene
- Details on test material:
- IUCLID4 Test substance: as prescribed by 1.1 - 1.4
MCS 2313
Purity:Not stated
Density 0.8705 g/ml
Reference No.: NBP 3494320
Appearance: Clear colorless liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Source: Charles River Breeding Laboratories Wilmington, Massachusetts - Sprague-Dawley rats - Age: 9 to 12 weeks of age - Weight at study initiation: 292 to 355 grams for males, and 224 to 253 grams for females
Observations : All animals were checked for viability twice daily. Prior to assignment to study all animals were examined to ascertain suitability for study.
Husbandry: Currently acceptable practices of good animal husbandry were followed, e.g. Guide for the Care and Use of Latoratory Animals: DHEH Publication No. (NIH) 78-23 Revised 1978
Housing: Group housing(six/cage) during equilibration. Individually housed during study.
Cages: Suspended, stainless steel with wire mesh bottoms.
Environmental Conditions: Temperature: 67-76F is considered an acceptable tmperature range for rats; room temperature was monitored and recorded twice daily and maintained within this range to the maximum extent possible.
Humidity: 30-70% is considered an acceptable humidity range for rats; room humidity was monitored and recorded daily and maintained within this range to the maximum extent possible.
Light Cycle: 12 hours light, 12 hours dark (controlled by an automatic timer).
Food:Purina Laboratory Chow, #5002, ad libittum
Water: Automatic watering system, ad libitum. Municipal water supply (Elizabethtown Water Co.)
Contaminants: There were no known contaminants reasonably expected to be found in food or water which would interfere with the results of this study.
Identification: Each animal was identified with a monel eat tag, bearing a unique number, prior to testing.
Selection: Animals were randimly placed in cages upon receipt, and were placed on study as available at the time of study initiation. Any animals considered unsuitable because of poor health or outlying body weights were excluded.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- The test material was administered by oral intubat!on, using a ball-tipped intubation needle fitted onto a syringe. Doses were calculated using fasted body weights.
- Doses:
- 1700, 2500, 3500 and 5000 mg/kg
- No. of animals per sex per dose:
- 5/sex/dose (Total- 20 rats)
- Control animals:
- no
- Details on study design:
- A slngle dose was administered to each animal followed by 14 days of observations. Following a range- finding study the definitive study was conducted with 5 animals/sex/dose given 1700, 2500, 3500 or 5000 mg/kg. Anlmals were fasted overnight ( for approximately 18 hours) prior to treatment. The material was admisistered as received; no preparation was necessary. The test material was administered by oral intubat!on, using a
ball-tipped intubation needle fitted onto a syringe. Doses were calculated using fasted body weights.
The following observations were made: viability checks- twice daily, observations of pharmacologic and toxicologic signs- ~1, 2, and 4 hours after dosing and daily thereafter for fourteen days, body weights- pre-fast, post fast (just prior to dosing, weights used for calculation of doses), day 7 and day 14. Gross postmortem examinations were performed on all animals which died or were found dead during the study. At termination of the observatio period (day 14), all surviving animals were killed by carbon dioxide inhalation and examined grossly. All abnormalities were recorded but no tissues were saved. - Statistics:
- None
Results and discussion
- Preliminary study:
- Not applicable
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 2 050 mL/kg bw
- Based on:
- test mat.
- 95% CL:
- 1 770 - 2 330
- Mortality:
- See below
- Clinical signs:
- other: other: A variety of abnormal signs occurred on the day of dosing. Several animals exhibited hypoactivity, red nasal discharge, urinary staining, partially closed eyes, prostration, and decreased food consumption. Signs seen in a few animals (in most
- Gross pathology:
- Postmortem examinations of animals, which were found dead, revealed a variety of changes, primarily blue pigmentation of all/most soft tissues and/or blue fluid in the gastrointestinal tract and urinary bladder. Other changes seen in most animals, which were found dead included changes in the stomach, intestine and urinary bladder which were suggestive of an irritant and/or corrosive effect (red or black walls, the presence of red,brown, or black fluid), discoloration of lungs, accentuated lobular pattern of the liver and red fluid in the abdominal cavity. Other changes in animals found dead appeared to represent autolytic changes or antemortem stress (testes in the body cavity). Changes in animals killed after 14 days included blue pigmentation of the brain and testes and dilated renal pelvis; other changes were simiiar to those seen in control animals in this laboratory killed by carbon dioxide inhalation or were considered to represent normal physiological variation.
- Other findings:
- None
Any other information on results incl. tables
Number of deaths:
mg/kg deaths (d; m/f)
1700 1/5 (d 3;m); 1/5 (d 6;f)
2500 5/5 (d 2;m); 4/5 (d 1-3;f)
3500 5/5 (d 1-3;m); 5/5 (d 1-5;f)
5000 5/5 (d 1-2;m); 5/5 (d 1-3;f)
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- LD50 value: = 2050 mg/kg (confidence range 1770 to 2330 mg/kg)
- Executive summary:
A single dose was administered to each animal followed by 14 days of observations. Following a range- finding study the definitive study was conducted with 5 animals/sex/dose given 1700, 2500, 3500 or 5000 mg/kg. Anlmals were fasted overnight ( for approximately 18 hours) prior to treatment. The material was admisistered as received; no preparation was necessary. The test material was administered by oral intubation, using a ball-tipped intubation needle fitted onto a syringe. Doses were calculated using fasted body weights.
The following observations were made: viability checks- twice daily, observations of pharmacologic and toxicologic signs- ~1, 2, and 4 hours after dosing and daily thereafter for fourteen days, body weights- pre-fast, post fast (just prior to dosing, weights used for calculation of doses), day 7 and day 14. Gross postmortem examinations were performed on all animals which died or were found dead during the study. At termination of the observatio period (day 14), all surviving animals were killed by carbon dioxide inhalation and examined grossly. All abnormalities were recorded but no tissues were saved.
The acute oral LD50 was determined to be 2050 mg/kg (confidence range 1770 to 2330 mg/kg) in rats.
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